JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB303741

Human Hsp27 ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human Hsp27 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Hsp27 with a sensitivity of 11.702 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

HSP27, HSP28, HSPB1, Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, Heat shock protein family B member 1, Stress-responsive protein 27, HSP 27, SRP27

3 Images
Sandwich ELISA - Human Hsp27 ELISA Kit (AB303741)
  • sELISA

Supplier Data

Sandwich ELISA - Human Hsp27 ELISA Kit (AB303741)

Interpolated concentrations of Hsp27 in normal human serum donors

Serum of ten individual healthy human gender donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean Hsp27 concentration was determined to be 1,080 pg/mL with a range of 650 – 1,660 pg/mL.

Sandwich ELISA - Human Hsp27 ELISA Kit (AB303741)
  • sELISA

Supplier Data

Sandwich ELISA - Human Hsp27 ELISA Kit (AB303741)

Interpolated concentrations of human Hsp27 in serum, plasma (citrate), plasma (EDTA), and plasma (heparin)

Interpolated concentration of native Hsp27 was measured in duplicate at different sample concentrations. Undiluted samples are 100% serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Hsp27 ELISA Kit (AB303741)
  • sELISA

Supplier Data

Sandwich ELISA - Human Hsp27 ELISA Kit (AB303741)

Example of human Hsp27 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, Heparin Plasma, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 11.702 pg/mL

Range

58.594 - 3750 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Assay Kits

AB181421

Human TNF alpha ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human TNF alpha ELISA Kit (AB181421)

  • 5
  • 4
Assay Kits

AB178013

Human IL-6 ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human IL-6 ELISA Kit (AB178013)

  • 5
  • 2
Assay Kits

AB214025

Human IL-1 beta ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human IL-1 beta ELISA Kit (AB214025)

  • 4
  • 1
Primary Antibodies

AB109376

Anti-Hsp27 antibody [EPR5477]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-Hsp27 antibody [EPR5477] (AB109376)

  • 5
  • 2
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human Hsp27 SimpleStep ELISA® kit (ab303741) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Hsp27 protein in serum, plasma (heparin), plasma (EDTA), plasma (citrate). Quantitate Human Hsp27 with 11.702 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.3" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.8" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "93 - 103 %", "average": "= 100" }, { "sample": "Citrate plasma", "range": "101 - 115 %", "average": "= 110" }, { "sample": "EDTA Plasma", "range": "80 - 89 %", "average": "= 84" }, { "sample": "Heparin Plasma", "range": "85 - 115 %", "average": "= 102" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab303741-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB303741-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB303741-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB303741-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB303741-CMP11", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB303741-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Hsp27 Capture Antibody", "number":"AB303741-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Hsp27 Lyophilized Recombinant Protein", "number":"AB303741-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Hsp27 Detector Antibody", "number":"AB303741-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB303741-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB303741-CMP06", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hsp27 also known as HSPB1 is a small heat shock protein with a molecular weight of approximately 27 kilodaltons. This protein is expressed in various tissues including muscle heart and brain. It functions as a molecular chaperone that stabilizes unfolded proteins preventing their aggregation. Hsp27 undergoes phosphorylation at specific residues which modulates its chaperone activity and interaction with other proteins.
Biological function summary

Hsp27 plays a critical role in cellular stress response by regulating actin cytoskeleton dynamics and inhibiting apoptosis. It forms part of a complex that includes other proteins such as alphaB-crystallin. This complex facilitates the reorganization of proteins under stress conditions enhancing cell survival during oxidative stress or thermal shock. Hsp27 also modulates inflammatory responses and has been shown to affect cell migration.

Pathways

Hsp27 integrates into the apoptosis and inflammation pathways. It interacts with apoptotic machinery such as caspase proteins to protect cells by hindering apoptosome formation. Additionally Hsp27 can engage with pathways involving the nuclear factor-kappa B (NF-kB) impacting inflammatory signaling. CPTC (carboxyl-pyrene-trioctylamine) can modulate these pathways by altering Hsp27 function and interactions.

Hsp27 has connections to neurodegenerative diseases and cancer. In neurodegenerative conditions such as Alzheimer's disease its chaperone activity is thought to protect neurons from misfolded protein aggregates. In cancer Hsp27 supports tumor cell survival and resistance to chemotherapy by interacting with proteins like Akt and p53. These interactions highlight the complex role of Hsp27 in modulating cellular responses in various pathological states.
style
left-column

Product protocols

style
left-column

Target data

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed : 10383393, PubMed : 20178975). Plays a role in stress resistance and actin organization (PubMed : 19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed : 23728742).
See full target information HSPB1
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com