Human HSP60 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human HSP60 in Human in Tissue Extracts, Cell Lysate samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts, Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix (PubMed:11422376, PubMed:1346131). The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein (Probable).
Alternative names
HSP60, HSPD1, 60 kDa chaperonin, Chaperonin 60, Heat shock protein 60, Heat shock protein family D member 1, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, CPN60, HSP-60, Hsp60
What's included?
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Human HSP60 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human HSP60 in Human in Tissue Extracts, Cell Lysate samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts, Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Assay Platform
- Microplate
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 4 | mean - | SD - | C.V. 2.1 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 3 | mean - | SD - | C.V. 6.7 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
ab124534 HSP60 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of HSP60 protein a 60 kDa protein encoded by the Human HSPD1 gene, in cell and tissue lysates. The assay employs an HSP60 specific antibody coated onto well plate strips.
Standards and samples are pipetted into the wells and analyte present in the sample is bound to the wells by the immobilized antibody. The wells are washed and an anti-HSP60 primary detector antibody is added. After washing away unbound primary detector antibody, HRP-conjugated secondary detector antibody specific for the primary detector antibody is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of analyte bound. The developing blue color is measured at 600 nm.
Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.
Species– Human reactive. Non-reactive with mouse and rat. Others untested.
Typical working ranges:
HepG2 cell extract: 0.39 - 200 µg/mL
Hela cell extract: 3.125 - 200 µg/mL
Store all components at 4°C. This kit is stable for at least 6 months from receipt. Unused microplate strips should be returned to the pouch containing the desiccant and resealed.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HSP60 also known as Heat Shock Protein 60 or Chaperonin 60 is a mitochondrial protein with a mass of about 60 kDa. It plays a critical role in protein folding and maintenance inside mitochondria. HSP60 is highly conserved across many species underlining its importance in cellular processes. Apart from mitochondria HSP60 is also present in cytosol and extracellular environments where it continues to exhibit chaperoning functions.
- Biological function summary
HSP60 interacts with newly synthesized proteins preventing their aggregation and assisting in proper folding. It forms complexes with co-chaperonin proteins such as HSP10 contributing to its function. This dual complex ensures the correct assembly of mitochondrial proteins important for cellular respiration and energy production. HSP60 further assists in refolding of damaged proteins maintaining cellular proteostasis.
- Pathways
HSP60 holds an important position in mitochondrial protein import and folding pathways. It acts in conjunction with other heat shock proteins such as HSP70 to facilitate translocation of proteins into mitochondria and assist in their folding once inside. HSP60 also integrates into the stress response pathway where it upregulates in response to cellular stressors aiding in the adaptation to altered physiological conditions.
- Associated diseases and disorders
HSP60 is linked to a variety of conditions including neurodegenerative disorders like Alzheimer's disease and cardiovascular diseases such as atherosclerosis. Alterations in its expression or function can lead to accumulation of misfolded proteins contributing to cell dysfunction and disease pathology. HSP60 also interacts with apoptotic proteins like BAX influencing cell survival and death pathways which may impact disease progression or resolution.
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8 product images
Flow Cytometry - Human HSP60 ELISA Kit (ab124534)
Analysis of HSP60 staining in Hela cells by Flow: blue: 1µg/mL HSP60 antibody; red: an equal amount of an isotype control antibody.
Immunocytochemistry/ Immunofluorescence - Human HSP60 ELISA Kit (ab124534)
Analysis of HSP60 staining in HDFn cells by ICC: HSP60 primary antibody at 1 µg/mL (green).
Western blot - Human HSP60 ELISA Kit (ab124534)
Western blot using the HSP60 detector antibody at 1µg/mL demonstrates assay specificity. HepG2 cell lysate loaded at 15µg/lane.
Sandwich ELISA - Human HSP60 ELISA Kit (ab124534)
Sensitivity of HSP60 ELISA kit is used to demonstrate the quantitative differentiation among different human cell lines.
Sandwich ELISA - Human HSP60 ELISA Kit (ab124534)
Detection of endogenous HSP60 levels in different cell lysates using HSP60 ELISA kit.
Western blot - Human HSP60 ELISA Kit (ab124534)
Detection of endogenous HSP60 levels in different cell lysates using Western blot. HSP60 antibody at 1µg/mL: HepG2 cell lysate (lane 2), HeLa cell lysate (lane 3), and HDFn lysate (lane 4), each were loaded at 15µg/lane.
Sandwich ELISA - Human HSP60 ELISA Kit (ab124534)
Example standard curve of serially titrated Hela cell lysate in the working range of the assay.
Sandwich ELISA - Human HSP60 ELISA Kit (ab124534)
Example standard curve of serially titrated HepG2 cell lysate in the working range of the assay.
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