Human I-309 ELISA Kit (CCL1) is a Sandwich (quantitative) ELISA kit for the measurement of Human I-309 (CCL1) in Human in Plasma, Cell culture supernatant samples.
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- The American journal of pathology 191:686-7032021Chemokine (C-C Motif) Ligand 1 Derived from Tumor-Associated Macrophages Contributes to Esophageal Squamous Cell Carcinoma Progression via CCR8-Mediated Akt/Proline-Rich Akt Substrate of 40 kDa/Mammalian Target of Rapamycin Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMasataka Fujikawa,Yu-Ichiro Koma,Masayoshi Hosono,Naoki Urakawa,Kohei Tanigawa,Masaki Shimizu,Takayuki Kodama,Hiroki Sakamoto,Mari Nishio,Manabu Shigeoka,Yoshihiro Kakeji,Hiroshi YokozakiPubMed 33460563
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 4.12 - 3000 pg/mL
- Sensitivity
- < 4 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cytokine that is chemotactic for monocytes but not for neutrophils. Binds to CCR8.
Alternative names
SCYA1, CCL1, C-C motif chemokine 1, Small-inducible cytokine A1, T lymphocyte-secreted protein I-309
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Human I-309 ELISA Kit (CCL1) is a Sandwich (quantitative) ELISA kit for the measurement of Human I-309 (CCL1) in Human in Plasma, Cell culture supernatant samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 4.12 - 3000 pg/mL
- Assay Platform
- Microplate
- Sensitivity
- < 4 pg/mL
- General recovery
- 95%
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 79.9 | Range 70 - 103 % |
Sample type Plasma | Average % = 97.65 | Range 88 - 110 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Abcam's I-309 (CCL-1) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human I-309 (CCL-1) in plasma and cell culture supernatants. (Human I-309 concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for Human I-309 coated on a 96-well plate. Standards and samples are pipetted into the wells and I-309 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human I-309 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of I-309 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect the endogenous Human I-309 in normal serum with ab100536, only in serum spiked with Human I-309.
Optimization may be required with urine samples
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The CCL1 protein also known as I-309 protein is a cytokine that plays a significant role in the immune system. Its molecular mass is approximately 10 kDa. CCL1 is secreted by activated T cells and is found extensively in the thymus. It binds to the CCR8 receptor which suggests its involvement in immune signaling. This protein belongs to the CC chemokine family which is characterized by the presence of two adjacent cysteines near the amino terminus.
- Biological function summary
CCL1 influences immune cell trafficking and activation. It attracts monocytes T lymphocytes and some types of dendritic cells to sites of inflammation. Although it is not part of a large protein complex CCL1 works with other chemokines to modulate the immune response contributing to both innate and adaptive immune mechanisms. Its expression may increase or decrease depending on the immune needs of the body.
- Pathways
Studies show that CCL1 participates in the chemokine signaling pathway and the effector T-cell pathway. Within the chemokine signaling pathway CCL1 interacts with other chemokines like CCL17 and proteins involved in T-cell migration. This pathway helps coordinate the movement of immune cells to sites of inflammation or injury ensuring an effective immune response.
- Associated diseases and disorders
Research links CCL1 to conditions such as asthma and rheumatoid arthritis. In asthma CCL1 levels influence the recruitment of immune cells to the lungs contributing to inflammation and airway hyper-responsiveness. Similarly in rheumatoid arthritis its interaction with CCR8 has been associated with synovial inflammation. These diseases indicate that CCL1’s role in immune cell migration can have pathological consequences when dysregulated.
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Sandwich ELISA - Human I-309 ELISA Kit (CCL1) (ab100536)
Representative standard curve using ab100536
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Product protocols
- Visit the general protocols
- Visit troubleshooting
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