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Human I-309 ELISA Kit (CCL1) is a Sandwich (quantitative) ELISA kit for the measurement of Human I-309 (CCL1) in Human in Plasma, Cell culture supernatant samples.
Alternative names=C-C motif chemokine 1, Small-inducible cytokine A1, T lymphocyte-secreted protein I-309, CCL1, SCYA1
- Detection method
Colorimetric
- Sample types
Plasma, Cell culture supernatant
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
4.12 - 3000 pg/mL
- Sensitivity
< 4 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Human I-309 ELISA Kit (CCL1) is a Sandwich (quantitative) ELISA kit for the measurement of Human I-309 (CCL1) in Human in Plasma, Cell culture supernatant samples.
Alternative names=C-C motif chemokine 1, Small-inducible cytokine A1, T lymphocyte-secreted protein I-309, CCL1, SCYA1
Key facts
- Detection method
Colorimetric
- Sample types
Plasma, Cell culture supernatant
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
4.12 - 3000 pg/mL
- Assay Platform
Microplate
- Sensitivity
< 4 pg/mL
- General recovery
95%
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 79.9 | Range 70 - 103 % |
Sample type Plasma | Average % = 97.65 | Range 88 - 110 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
-20°C
Notes
Abcam's I-309 (CCL-1) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human I-309 (CCL-1) in plasma and cell culture supernatants. (Human I-309 concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for Human I-309 coated on a 96-well plate. Standards and samples are pipetted into the wells and I-309 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human I-309 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of I-309 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect the endogenous Human I-309 in normal serum with ab100536, only in serum spiked with Human I-309.
Optimization may be required with urine samples
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