Human ICAM1 Catchpoint ELISA Kit - Extracellular is a single-wash 90-min Simplestep used to quantify Human ICAM1 Catchpoint with a sensitivity of 14.063 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum, Urine, Citrate plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 34.38 - 2200 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 14.063 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. (Microbial infection) Acts as a receptor for major receptor group rhinovirus A-B capsid proteins. (Microbial infection) Acts as a receptor for Coxsackievirus A21 capsid proteins. (Microbial infection) Upon Kaposi's sarcoma-associated herpesvirus/HHV-8 infection, is degraded by viral E3 ubiquitin ligase MIR2, presumably to prevent lysis of infected cells by cytotoxic T-lymphocytes and NK cell.
Alternative names
CD54, Intercellular adhesion molecule 1, ICAM-1, Major group rhinovirus receptor, ICAM1
What's included?
Recommended products
Human ICAM1 Catchpoint ELISA Kit - Extracellular is a single-wash 90-min Simplestep used to quantify Human ICAM1 Catchpoint with a sensitivity of 14.063 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum, Urine, Citrate plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 34.38 - 2200 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 14.063 pg/mL
- General recovery
- 91%
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. 0.1 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 0.031 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 90 | Range 89 - 91 % |
Sample type Citrate plasma | Average % = 83 | Range 76 - 92 % |
Sample type EDTA Plasma | Average % = 89 | Range 85 - 95 % |
Sample type Heparin Plasma | Average % = 94 | Range 87 - 101 % |
Sample type Serum | Average % = 91 | Range 81 - 100 % |
Sample type Urine | Average % = 95 | Range 89 - 101 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human ICAM1 - Extracellular Catchpoint SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ICAM1 - Extracellular Catchpoint protein in human serum, Heparin plasma, EDTA plasma, urine and cell culture supernatant. Quantitate Human ICAM1 - Extracellular Catchpoint with 14.063 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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5 product images
Sandwich ELISA - Human ICAM1 Catchpoint ELISA Kit - Extracellular (ab309184)
Standard curve comparison between the original Human ICAM1 Catchpoint SimpleStep ELISA (Human ICAM1 ELISA Kit, Fluorescent ab229383) and current Human ICAM1 Catchpoint SimpleStep ELISA (ab309184)
Sandwich ELISA - Human ICAM1 Catchpoint ELISA Kit - Extracellular (ab309184)
Interpolated concentration of native ICAM1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows: urine 50%, 1.5 % PHA-M 24 hours stimulated PBMC supernatant 30%, and unstimulated PBMC supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human ICAM1 Catchpoint ELISA Kit - Extracellular (ab309184)
Example of human ICAM1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human ICAM1 Catchpoint ELISA Kit - Extracellular (ab309184)
Interpolated concentration of native ICAM1 was measured in duplicate at different sample concentrations. Undiluted samples are 1:200 serum, plasma (heparin), plasma (EDTA) and plasma (citrate). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human ICAM1 Catchpoint ELISA Kit - Extracellular (ab309184)
Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean ICAM1 concentration was determined to be 226 ng/mL with a range of 158 – 268 ng/mL.
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Product protocols
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