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AB100640

Human ICAM1 ELISA Kit (CD54)

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(7 Publications)

Human ICAM1 ELISA Kit (CD54) is a sandwich ELISA designed to quantify Human ICAM1 (CD54) with a sensitivity of 150 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 150 - 20000 pg/mL

View Alternative Names

CD54, Intercellular adhesion molecule 1, ICAM-1, Major group rhinovirus receptor, ICAM1

2 Images
Sandwich ELISA - Human ICAM1 ELISA Kit (CD54) (AB100640)
  • sELISA

Unknown

Sandwich ELISA - Human ICAM1 ELISA Kit (CD54) (AB100640)

Representative standard curve using ab100640

Sandwich ELISA - Human ICAM1 ELISA Kit (CD54) (AB100640)
  • sELISA

Unknown

Sandwich ELISA - Human ICAM1 ELISA Kit (CD54) (AB100640)

Representative standard curve using ab100640

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 150 pg/mL

Range

150 - 20000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human ICAM1 ELISA Kit (CD54) ab100640 is a sandwich ELISA to measure Human ICAM1 (CD54) in serum, plasma, cell culture supernatant with a sensitivity of 150 pg/ml.

How the assay works

This assay employs an antibody specific for Human ICAM1 coated on a 96-well plate. Standards and samples are pipetted into the wells and ICAM1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human ICAM1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ICAM1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Get higher sensitivity in only 90 minutes with Human ICAM1 ELISA Kit (CD54) (ab174445) from our SimpleStep ELISA® range.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human ICAM1 ELISA Kit (CD54) ab100640 protocol summary

1. Add standard or sample to each well used. Incubate at room temperature
2. Wash and add prepared biotinylated antibody to each well. Incubate at room temperature.
3. Wash and add prepared streptavidin solution. Incubate at room temperature.
4. Add TMB Development Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read at 450nm immediately.

Get results in 90 minutes with our SimpleStep ELISA® kit

Human ICAM1 ELISA Kit (CD54) (ab174445)

Optimization may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "89 - 107 %", "average": "= 97.37" }, { "sample": "Serum", "range": "88 - 106 %", "average": "= 97.48" }, { "sample": "Plasma", "range": "90 - 107 %", "average": "= 98.67" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100640-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100640-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100640-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"ICAM1 Microplate (12 x 8 wells)", "number":"AB100640-CMP06", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100640-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human ICAM1", "number":"AB100640-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100640-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human ICAM1 Standard (lyophilized)", "number":"AB100640-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100640-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"340X HRP-Streptavidin Concentrate", "number":"AB100640-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation.. (Microbial infection) Acts as a receptor for major receptor group rhinovirus A-B capsid proteins.. (Microbial infection) Acts as a receptor for Coxsackievirus A21 capsid proteins.. (Microbial infection) Upon Kaposi's sarcoma-associated herpesvirus/HHV-8 infection, is degraded by viral E3 ubiquitin ligase MIR2, presumably to prevent lysis of infected cells by cytotoxic T-lymphocytes and NK cell.
See full target information ICAM1
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceutics 14: PubMed35057008

2022

Elovanoids Counteract Inflammatory Signaling, Autophagy, Endoplasmic Reticulum Stress, and Senescence Gene Programming in Human Nasal Epithelial Cells Exposed to Allergens.

Applications

Unspecified application

Species

Unspecified reactive species

Alfredo Resano,Surjyadipta Bhattacharjee,Miguel Barajas,Khanh V Do,Roberto Aguado-Jiménez,David Rodríguez,Ricardo Palacios,Nicolás G Bazán

Journal of infection in developing countries 15:289-296 PubMed33690213

2021

Cardiovascular risks in Asian HIV-infected patients receiving boosted-protease inhibitor-based antiretroviral treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Sakaewan Ounjaijean,Kanokwan Kulprachakarn,Linda Aurpibul,Quanhathai Kaewpoowat,Kongsak Boonyapranai,Romanee Chaiwarith,Supapong Arwon,Khuanchai Supparatpinyo,Kittipan Rerkasem

Journal of extracellular vesicles 9:1801153 PubMed32944190

2020

(Sub)populations of extracellular vesicles released by TNF-α -triggered human endothelial cells promote vascular inflammation and monocyte migration.

Applications

Unspecified application

Species

Unspecified reactive species

Baharak Hosseinkhani,Nynke M S van den Akker,Daniel G M Molin,Luc Michiels

Scientific reports 8:1762 PubMed29379127

2018

PlanHab Study: Consequences of combined normobaric hypoxia and bed rest on adenosine kinetics.

Applications

Unspecified application

Species

Unspecified reactive species

C Strewe,R Zeller,M Feuerecker,M Hoerl,S Matzel,I Kumprej,A Crispin,B Johannes,T Debevec,I B Mekjavic,O Eiken,M Thiel,G Schelling,A Choukèr

Evidence-based complementary and alternative medic 2016:6134593 PubMed28050193

2016

Herbal Medicine Improves Health-Related Quality of Life in Moderate-to-Severe Asthma.

Applications

Unspecified application

Species

Unspecified reactive species

Ningqun Wang,Jie Li,Xiaobo Huang,Wenqiang Chen,Yujing Chen

TheScientificWorldJournal 2013:387849 PubMed24319364

2013

Emergent biomarkers of residual cardiovascular risk in patients with low HDL-c and/or high triglycerides and average LDL-c concentrations: focus on HDL subpopulations, Oxidized LDL, adiponectin, and uric acid.

Applications

Unspecified application

Species

Unspecified reactive species

Filipa Mascarenhas-Melo,Filipe Palavra,Daniela Marado,José Sereno,Edite Teixeira-Lemos,Isabel Freitas,Maria Isabel-Mendonça,Rui Pinto,Frederico Teixeira,Flávio Reis

Cardiovascular diabetology 12:61 PubMed23570342

2013

Diabetes abrogates sex differences and aggravates cardiometabolic risk in postmenopausal women.

Applications

Unspecified application

Species

Unspecified reactive species

Filipa Mascarenhas-Melo,Daniela Marado,Filipe Palavra,José Sereno,Álvaro Coelho,Rui Pinto,Edite Teixeira-Lemos,Frederico Teixeira,Flávio Reis
View all publications
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websiteProtocolBooklet
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