Human ICAM1 ELISA Kit (CD54)

Product details

Human ICAM1 ELISA Kit (CD54) ab174445 is a rapid single-wash 90-min Sandwich ELISA to measure Human ICAM1 (CD54)in cell culture extracts, cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 1.6 pg/mL.

How the assay works

Human ICAM1 (CD54)SimpleStep ELISA^®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human ICAM1 ELISA Kit (CD54) ab174445 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

ICAM1, is a cell surface glycoprotein typically expressed in endothelial cells and cells of the immune system. The extracellular portion of ICAM-1 forms five immunoglobulin like domains attached to a single hydrophobic transmembrane region and a short cytoplasmic tail. ICAM-1, binds to the Leukocyte Integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) as well as to non integrin ligands such as CD43, fibrinogen, hyaluronan, Rhinoviruses and Plasmodium falciparum-infected erythrocytes. Binding to LFA-1 facilitates trans-endothelial leukocyte migration to areas of inflammation via promotion of endothelial apical cups assembly.

ICAM-1 expression increases in the vascular endothelium, macrophages and lymphocytes in response to cytokine stimulation (IL-1 and TNF). Furthermore, ligation of ICAM-1 up-regulates its own expression in a positive-feedback loop to maintain a pro-inflammatory environment conducive to leukocytes endothelial transmigration.

Soluble ICAM-1 is reported to be present in normal serum, cerebrospinal fluid, urine and bronchoalveolar lavage fluid. Elevated levels of soluble ICAM-1 have been associated with multiple organ failure, atherosclerosis, autoimmune disorders, diabetes, obesity, hypertension, liver disease as well as cancer, and several studies have correlated serum levels of sICAM-1 with the severity of these diseases.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.