Human ICAM1 ELISA Kit (CD54) is a single-wash 90-min Simplestep used to quantify Human ICAM1 (CD54) with a sensitivity of 1.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Cited in over 25 citations
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- Heliyon 9:e173662023circSnd1 promotes atherosclerosis progression through the miR-485-3p/Olr1 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLin Yang,Yuhao Lin,Chao Wang,Pengcheng FanPubMed 37426804
- Journal of applied toxicology : JAT 43:1613-16292023Integrative proteomics and metabolomics analysis of non-observable acute effect level PM induced accumulative effects in AC16 cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBingru Nan,Xia Sun,Shijing Yang,Qingyu Huang,Heqing ShenPubMed 37278136
- Redox biology 62:1026812023MiR-27b attenuates mitochondrial oxidative stress and inflammation in endothelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNunzia D'Onofrio,Francesco Prattichizzo,Elisa Martino,Camilla Anastasio,Luigi Mele,Rosalba La Grotta,Celestino Sardu,Antonio Ceriello,Raffaele Marfella,Giuseppe Paolisso,Maria Luisa BalestrieriPubMed 37003179
- Medicine 102:e323842023Effects of amlodipine combined with atorvastatin on Th17/Treg imbalance and vascular microcirculation in hypertensive patients with atherosclerosis: A double-blind, single-center randomized controlled trial.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGui Yang,Youjiang QiuPubMed 36820569
- Frontiers in immunology 13:10908772022Piperlongumine alleviates corneal allograft rejection suppressing angiogenesis and inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiangyu Fan,Jini Qiu,Tianjie Yuan,Jing Zhang,Jianjiang XuPubMed 36591243
- Radiology and oncology 56:501-5072022Plasma sICAM-1 correlates with tumor volume before primary radiochemotherapy of head and neck squamous cell carcinoma patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKerstin Clasen,Stefan Welz,Heidrun Faltin,Daniel Zips,Franziska EckertPubMed 36503712
- Oxidative medicine and cellular longevity 2021:81894852021Autophagic Flux Unleashes GATA4-NF-B Axis to Promote Antioxidant Defense-Dependent Survival of Colorectal Cancer Cells under Chronic Acidosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaojie Liu,Minnan Zhao,Xue Sun,Zhenzhen Meng,Xiaojing Bai,Yanchao Gong,Limei Xu,Xiaohe Hao,Tingting Yang,Zhao Wei,Xiyu Zhang,Haiyang Guo,Peishan Li,Qiao Liu,Yaoqin Gong,Yufang Shi,Changshun ShaoPubMed 34987705
- Journal of clinical medicine 10:2021Evaluation of the Differences in the Expression of Biogenic Amine-Related mRNAs and Proteins in Endometrioid Endometrial Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichał Czerwiński,Anna Bednarska-Czerwińska,Nikola Zmarzły,Dariusz Boroń,Marcin Oplawski,Beniamin Oskar GrabarekPubMed 34768392
- Frontiers in cardiovascular medicine 8:6829592021Correlation of Long Non-coding RNA LncRNA-FA2H-2 With Inflammatory Markers in the Peripheral Blood of Patients With Coronary Heart Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFengxia Guo,Yanhua Sha,Bing Hu,Gang LiPubMed 34235188
- Neuroimmunomodulation 28:266-2752021Activation of Insulin-Like Growth Factor-2 Ameliorates Retinal Cell Damage and Exerts Protection in in vitro Model of Diabetic Retinopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYantao Zhao,Zhaohui Xiong,Yuping Chen,Guoqiang Wang,Yan ZhaoPubMed 33951651
Key facts
- Detection method
- Colorimetric
- Sample types
- Adherent cells, Cell culture extracts, Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 39.06 - 2500 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 1.6 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. (Microbial infection) Acts as a receptor for major receptor group rhinovirus A-B capsid proteins. (Microbial infection) Acts as a receptor for Coxsackievirus A21 capsid proteins. (Microbial infection) Upon Kaposi's sarcoma-associated herpesvirus/HHV-8 infection, is degraded by viral E3 ubiquitin ligase MIR2, presumably to prevent lysis of infected cells by cytotoxic T-lymphocytes and NK cell.
Alternative names
CD54, Intercellular adhesion molecule 1, ICAM-1, Major group rhinovirus receptor, ICAM1
What's included?
Recommended products
Human ICAM1 ELISA Kit (CD54) is a single-wash 90-min Simplestep used to quantify Human ICAM1 (CD54) with a sensitivity of 1.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Cited in over 25 citations
Key facts
- Detection method
- Colorimetric
- Sample types
- Adherent cells, Cell culture extracts, Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 39.06 - 2500 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Microplate
- Sensitivity
- = 1.6 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 6 | C.V. 6 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 24 | C.V. 7 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 103 | Range 99 - 110 % |
Sample type Cell culture media | Average % = 98 | Range 91 - 102 % |
Sample type Extraction Buffer | Average % = 87 | Range 82 - 97 % |
Sample type Plasma | Average % = 113 | Range 96 - 126 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human ICAM-1 ELISA kit (CD54) (ab174445) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ICAM-1 protein in human cell culture supernatant, plasma, serum and cell lysate samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate human ICAM-1 with 1.6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.
ICAM1, is a cell surface glycoprotein typically expressed in endothelial cells and cells of the immune system. The extracellular portion of ICAM-1 forms five immunoglobulin like domains attached to a single hydrophobic transmembrane region and a short cytoplasmic tail. ICAM-1, binds to the Leukocyte Integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) as well as to non integrin ligands such as CD43, fibrinogen, hyaluronan, Rhinoviruses and Plasmodium falciparum-infected erythrocytes. Binding to LFA-1 facilitates trans-endothelial leukocyte migration to areas of inflammation via promotion of endothelial apical cups assembly.
ICAM-1 expression increases in the vascular endothelium, macrophages and lymphocytes in response to cytokine stimulation (IL-1 and TNF). Furthermore, ligation of ICAM-1 up-regulates its own expression in a positive-feedback loop to maintain a pro-inflammatory environment conducive to leukocytes endothelial transmigration.
Soluble ICAM-1 is reported to be present in normal serum, cerebrospinal fluid, urine and bronchoalveolar lavage fluid. Elevated levels of soluble ICAM-1 have been associated with multiple organ failure, atherosclerosis, autoimmune disorders, diabetes, obesity, hypertension, liver disease as well as cancer, and several studies have correlated serum levels of sICAM-1 with the severity of these diseases.
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5 product images
Sandwich ELISA - Human ICAM1 ELISA Kit (CD54) (ab174445)
Example ICAM-1 standard curve.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human ICAM1 ELISA Kit (CD54) (ab174445)
Example of ICAM-1 dynamic range in Raji cell lysates.
The curve was prepared by loading 5 μg/mL of Raji cell extracts, followed by a 1:2 titration series. Background-subtracted data values (mean +/- SD) are graphed.
ELISA - Human ICAM1 ELISA Kit (CD54) (ab174445)
Specificity of ICAM-1 signal on stimulated and non stimulated media supernatants.
Human PBMCs were cultured in RPMI supplemented with 10% fetal calf serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin. Cells were cultured for 2 days at 37⁰C in the presence or absence of PHA. The concentrations of ICAM-1 were interpolated from the calibration curve and corrected for sample dilution. The mean ICAM-1 concentration was determined to be 320 pg/mL in unstimulated PBMC supernatants and 2,654 pg/mL in stimulated PBMC supernatants.
ELISA - Human ICAM1 ELISA Kit (CD54) (ab174445)
Frequency histogram of ICAM-1 levels in serum of individual normal healthy donors.
The levels of ICAM-1 in serum samples were tested from ten individual healthy donors. Levels were interpolated from the standard curve and corrected for sample dilution. The levels of ICAM-1 are shown for the percentage of individuals within each 100 ng/mL bin center of the distribution. The mean level of ICAM-1 was 469 ng/mL with a range of 347 to 629 ng/mL and a standard deviation of 77 ng/mL.
ELISA - Human ICAM1 ELISA Kit (CD54) (ab174445)
Comparison of ICAM-1 levels in three Human cell culture lysates.
The levels of ICAM-1 protein were assessed in three Human cell line lysates loaded at 2.5 μg/mL of protein. The raw OD 450 nm signal for each sample was interpolated from an ICAM1 standard curve.
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