AB326011

Human ICAM1 ELISA Kit, Chemiluminescent- Extracellular

Recombinant
SimpleStep Ignite™ ELISA
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Human ICAM1 ELISA Kit, Chemiluminescent- Extracellular is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human ICAM1 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 3.2 - 7000 pg/ml with a sensitivity of 1.25 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

View Alternative Names

CD54, Intercellular adhesion molecule 1, ICAM-1, Major group rhinovirus receptor, ICAM1

7 Images

Sandwich ELISA - Human ICAM1 ELISA Kit, Chemiluminescent- Extracellular (AB326011)

sELISA

Lab

Sandwich ELISA - Human ICAM1 ELISA Kit, Chemiluminescent- Extracellular (AB326011)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

sELISA

Lab

Sandwich ELISA - Human ICAM1 ELISA Kit, Chemiluminescent- Extracellular (AB326011)

Interpolated concentration of native ICAM1 was measured in duplicate at different sample concentrations. Undiluted samples are 50% human urine. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Lab

Sandwich ELISA - Human ICAM1 ELISA Kit, Chemiluminescent- Extracellular (AB326011)

Standard curve comparison between the Chemiluminescent Human ICAM1 SimpleStep ELISA and colorimetric Human ICAM1 SimpleStep ELISA (ab309172) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human ICAM1 ELISA Kit, Chemiluminescent- Extracellular (AB326011)

Interpolated concentration of native ICAM1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : 1.5 % PHA-M 24 hours stimulated PBMC supernatant (SN) 30%, and unstimulated PBMC supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Lab

Sandwich ELISA - Human ICAM1 ELISA Kit, Chemiluminescent- Extracellular (AB326011)

Dynamic range comparison between the Chemiluminescent Human ICAM1 SimpleStep ELISA, colorimetric Human ICAM1 SimpleStep ELISA (ab309172), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

sELISA

Lab

Sandwich ELISA - Human ICAM1 ELISA Kit, Chemiluminescent- Extracellular (AB326011)

Interpolated concentration of native ICAM1 was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 200 serum, plasma (heparin), plasma (EDTA) and plasma (citrate). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS

sELISA

Lab

Sandwich ELISA - Human ICAM1 ELISA Kit, Chemiluminescent- Extracellular (AB326011)

Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ICAM1 concentration was determined to be 226 ng/mL with a range of 158 – 268 ng/mL.

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant, Urine

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.25 pg/mL

Range

3.2 - 7000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human ICAM1 ELISA Kit, Chemiluminescent- Extracellular (ab326011) is a rapid single-wash sandwich 55-min ELISA to measure Human ICAM1 in Serum, Citrate Plasma, EDTA Plasma, Heparin Plasma, Cell Culture Supernatant, Urine with a sensitivity of 1.25 pg/mL.

How the assay works

Human ICAM1 ELISA Kit, Chemiluminescent- Extracellular (ab326011) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human ICAM1 (ab326011) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-ICAM-1 antibody [EPR24639-21] - BSA and Aside free (Capture) ab288605
- Anti-ICAM-1 antibody [EPR24639-179] - BSA and Aside free (Detector) ab288623

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.5" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.1" } ]

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Recovery

[ { "sample": "Serum", "range": "81 - 100 %", "average": "= 91" }, { "sample": "Heparin Plasma", "range": "87 - 101 %", "average": "= 94" }, { "sample": "Citrate plasma", "range": "76 - 92 %", "average": "= 83" }, { "sample": "EDTA Plasma", "range": "85 - 95 %", "average": "= 89" }, { "sample": "Urine", "range": "89 - 101 %", "average": "= 95" }, { "sample": "Cell culture supernatant", "range": "89 - 91 %", "average": "= 90" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab326011-1x96Tests", "assetComponentDetails": [ { "size":"1 x 600 µL", "name":"10X Human ICAM1 Capture Antibody", "number":"AB326011-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB326011-CMP10", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB326011-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB326011-CMP04", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB326011-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human ICAM1 Detector Antibody", "number":"AB326011-CMP02", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB326011-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB326011-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB326011-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human ICAM1 Lyophilized Recombinant Protein", "number":"AB326011-CMP03", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Cell adhesion molecule that functions as a receptor ligand of the signaling receptor ITGAL : ITGB2/LFA-1 (lymphocyte-function associated (LFA) molecule 1) ensuring leukocyte cell-cell adhesion, by providing a calibrated system to namely adjust T-cell killing to the antigen stimulation strength (PubMed : 3086451, PubMed : 3340213, PubMed : 38195629). Also functions as a ligand receptor of the signaling receptor ITGAM : ITGB2/MAC-1 ensuring adhesion between stimulated neutrophils and stimulated endothelial cells (PubMed : 1980124). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation (PubMed : 17875742). Promotes cell aggregation in epithelial cells through interaction with MUC1 (PubMed : 11173916).. (Microbial infection) Acts as a receptor for major receptor group rhinovirus A-B capsid proteins.. (Microbial infection) Acts as a receptor for Coxsackievirus A21 capsid proteins.. (Microbial infection) Upon Kaposi's sarcoma-associated herpesvirus/HHV-8 infection, is degraded by viral E3 ubiquitin ligase MIR2, presumably to prevent lysis of infected cells by cytotoxic T-lymphocytes and NK cell.

See full target information ICAM1

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websiteProtocolBooklet

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Product promise

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