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AB309172

Human ICAM1 ELISA Kit - Extracellular

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Human ICAM1 ELISA Kit - Extracellular is a single-wash 90-min Simplestep used to quantify Human ICAM1 with a sensitivity of 14.063 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD54, Intercellular adhesion molecule 1, ICAM-1, Major group rhinovirus receptor, ICAM1

6 Images
Sandwich ELISA - Human ICAM1 ELISA Kit - Extracellular (AB309172)
  • sELISA

Supplier Data

Sandwich ELISA - Human ICAM1 ELISA Kit - Extracellular (AB309172)

Interpolated concentrations of ICAM1 in normal human serum donors

Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean ICAM1 concentration was determined to be 226 ng/mL with a range of 158 – 268 ng/mL.

Sandwich ELISA - Human ICAM1 ELISA Kit - Extracellular (AB309172)
  • sELISA

Supplier Data

Sandwich ELISA - Human ICAM1 ELISA Kit - Extracellular (AB309172)

Interpolated concentrations of human ICAM1 in urine, PHA-M Stimulated and Unstimulated PBMC supernatants

Interpolated concentration of native ICAM1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : urine 50%, 1.5 % PHA-M 24 hours stimulated PBMC supernatant (SN) 30%, and unstimulated PBMC supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS

Sandwich ELISA - Human ICAM1 ELISA Kit - Extracellular (AB309172)
  • sELISA

Supplier Data

Sandwich ELISA - Human ICAM1 ELISA Kit - Extracellular (AB309172)

Interpolated concentrations of human ICAM1 in serum, plasma (heparin), plasma (EDTA) and plasma (citrate)

Interpolated concentration of native ICAM1 was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 200 serum, plasma (heparin), plasma (EDTA) and plasma (citrate). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS

Sandwich ELISA - Human ICAM1 ELISA Kit - Extracellular (AB309172)
  • sELISA

Supplier Data

Sandwich ELISA - Human ICAM1 ELISA Kit - Extracellular (AB309172)

Example of human ICAM1 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed

Sandwich ELISA - Human ICAM1 ELISA Kit - Extracellular (AB309172)
  • sELISA

Supplier Data

Sandwich ELISA - Human ICAM1 ELISA Kit - Extracellular (AB309172)

Standard curve comparison between the original Human ICAM1 SimpleStep ELISA (ab174445) and current Human ICAM1 SimpleStep ELISA (ab309172)

Sandwich ELISA - Human ICAM1 ELISA Kit - Extracellular (AB309172)
  • sELISA

Supplier Data

Sandwich ELISA - Human ICAM1 ELISA Kit - Extracellular (AB309172)

Standard curve comparison between Human ICAM1 SimpleStep ELISA kit (ab309172) and traditional ELISA kit from leading competitor. The SimpleStep ELISA kit shows increased sensitivity

Key facts

Detection method

Colorimetric

Sample types

Serum, Heparin Plasma, Citrate plasma, EDTA Plasma, Urine, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 14.063 pg/mL

Range

34.375 - 2200 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human ICAM1 - Extracellular SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ICAM1 - Extracellular protein in human serum, Heparin plasma, Citrate plasma, EDTA plasma, urine and cell culture supernatant. Quantitate Human ICAM1 - Extracellular with 14.063 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.5" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.1" } ]
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Recovery

[ { "sample": "Serum", "range": "81 - 100 %", "average": "= 91" }, { "sample": "Heparin Plasma", "range": "87 - 101 %", "average": "= 94" }, { "sample": "Citrate plasma", "range": "76 - 92 %", "average": "= 83" }, { "sample": "EDTA Plasma", "range": "85 - 95 %", "average": "= 89" }, { "sample": "Urine", "range": "89 - 101 %", "average": "= 95" }, { "sample": "Cell culture supernatant", "range": "89 - 91 %", "average": "= 90" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Cell adhesion molecule that functions as a receptor ligand of the signaling receptor ITGAL : ITGB2/LFA-1 (lymphocyte-function associated (LFA) molecule 1) ensuring leukocyte cell-cell adhesion, by providing a calibrated system to namely adjust T-cell killing to the antigen stimulation strength (PubMed : 3086451, PubMed : 3340213, PubMed : 38195629). Also functions as a ligand receptor of the signaling receptor ITGAM : ITGB2/MAC-1 ensuring adhesion between stimulated neutrophils and stimulated endothelial cells (PubMed : 1980124). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation (PubMed : 17875742). Promotes cell aggregation in epithelial cells through interaction with MUC1 (PubMed : 11173916).. (Microbial infection) Acts as a receptor for major receptor group rhinovirus A-B capsid proteins.. (Microbial infection) Acts as a receptor for Coxsackievirus A21 capsid proteins.. (Microbial infection) Upon Kaposi's sarcoma-associated herpesvirus/HHV-8 infection, is degraded by viral E3 ubiquitin ligase MIR2, presumably to prevent lysis of infected cells by cytotoxic T-lymphocytes and NK cell.
See full target information ICAM1
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websiteProtocolBooklet
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Product promise

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