Human ICAM1 ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Human ICAM1 with a sensitivity of 2.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader
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- Journal of clinical laboratory analysis 36:e246852022Serum VCAM-1 and ICAM-1 measurement assists for MACE risk estimation in ST-segment elevation myocardial infarction patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiancai Yu,Yongxing Liu,Wanzhong Peng,Zesheng XuPubMed 36045604
Key facts
- Detection method
- Fluorescent
- Sample types
- Adherent cells, Cell culture extracts, Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 4.9 - 20000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 2.4 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. (Microbial infection) Acts as a receptor for major receptor group rhinovirus A-B capsid proteins. (Microbial infection) Acts as a receptor for Coxsackievirus A21 capsid proteins. (Microbial infection) Upon Kaposi's sarcoma-associated herpesvirus/HHV-8 infection, is degraded by viral E3 ubiquitin ligase MIR2, presumably to prevent lysis of infected cells by cytotoxic T-lymphocytes and NK cell.
Alternative names
CD54, Intercellular adhesion molecule 1, ICAM-1, Major group rhinovirus receptor, ICAM1
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Recommended products
Human ICAM1 ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Human ICAM1 with a sensitivity of 2.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader
Key facts
- Detection method
- Fluorescent
- Sample types
- Adherent cells, Cell culture extracts, Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 4.9 - 20000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 2.4 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample General | n 6 | C.V. 6 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample General | n 24 | C.V. 7 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 103 | Range 99 - 110 % |
Sample type Cell culture media | Average % = 98 | Range 91 - 102 % |
Sample type Extraction Buffer | Average % = 87 | Range 82 - 97 % |
Sample type Plasma | Average % = 113 | Range 96 - 126 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
ICAM1 (CD54) in vitro CatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of ICAM1 (CD54) protein in human cell culture supernatant, plasma, serum, and cell extracts.
This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices' plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org
The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.
ICAM1, is a cell surface glycoprotein typically expressed in endothelial cells and cells of the immune system. The extracellular portion of ICAM-1 forms five immunoglobulin like domains attached to a single hydrophobic transmembrane region and a short cytoplasmic tail. ICAM-1, binds to the Leukocyte Integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) as well as to non-integrin ligands such as CD43, fibrinogen, hyaluronan, Rhinoviruses and Plasmodium falciparum-infected erythrocytes. Binding to LFA-1 facilitates trans-endothelial leukocyte migration to areas of inflammation via promotion of endothelial apical cups assembly.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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5 product images
Sandwich ELISA - Human ICAM1 ELISA Kit, Fluorescent (ab229383)
Example of human ICAM1 (CD54) standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human ICAM1 ELISA Kit, Fluorescent (ab229383)
Specificity of ICAM-1 signal on stimulated and unstimulated media supernatants.
Human PBMCs were cultured in RPMI supplemented with 10% fetal calf serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin. Cells were cultured for 2 days at 37⁰C in the presence or absence of PHA. The concentrations of ICAM-1 were interpolated from the calibration curve and corrected for sample dilution. The mean ICAM-1 concentration was determined to be 320 pg/mL in unstimulated PBMC supernatants and 2,654 pg/mL in stimulated PBMC supernatants.
Sandwich ELISA - Human ICAM1 ELISA Kit, Fluorescent (ab229383)
Frequency histogram of ICAM-1 levels in serum of individual normal healthy donors.
The levels of ICAM-1 in serum samples were tested from ten individual healthy donors. Levels were interpolated from the standard curve and corrected for sample dilution. The levels of ICAM-1 are shown for the percentage of individuals within each 100 ng/mL bin center of the distribution. The mean level of ICAM-1 was 469 ng/mL with a range of 347 to 629 ng/mL and a standard deviation of 77 ng/mL.
Sandwich ELISA - Human ICAM1 ELISA Kit, Fluorescent (ab229383)
Example of ICAM1 dynamic range in Raji cell extracts.
The curve was prepared by loading 5 μg/mL of Raji cell extracts, followed by a 1:2 titration series. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human ICAM1 ELISA Kit, Fluorescent (ab229383)
Comparison of ICAM-1 levels in three human cell culture lysates.
The levels of ICAM-1 protein were assessed in three human cell line lysates loaded at 2.5 μg/mL of protein. The raw OD 450nm signal for each sample was interpolated from an ICAM1 standard curve.
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