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AB309183

Human ICAM1 ELISA Kit - Intracellular

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Human ICAM1 ELISA Kit - Intracellular is a Sandwich (quantitative) ELISA for the measurement of Human ICAM1 - Intracellular in Human in Cell/Tissue Extracts samples.

View Alternative Names

CD54, Intercellular adhesion molecule 1, ICAM-1, Major group rhinovirus receptor, ICAM1

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Sandwich ELISA - Human ICAM1 ELISA Kit - Intracellular (AB309183)
  • sELISA

Supplier Data

Sandwich ELISA - Human ICAM1 ELISA Kit - Intracellular (AB309183)

Example of human ICAM1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human ICAM1 ELISA Kit - Intracellular (AB309183)
  • sELISA

Supplier Data

Sandwich ELISA - Human ICAM1 ELISA Kit - Intracellular (AB309183)

Standard curve comparison between the original Human ICAM1 SimpleStep ELISA (ab174445) and current Human ICAM1 SimpleStep ELISA(ab309183)

Sandwich ELISA - Human ICAM1 ELISA Kit - Intracellular (AB309183)
  • sELISA

Supplier Data

Sandwich ELISA - Human ICAM1 ELISA Kit - Intracellular (AB309183)

Interpolated concentration of native ICAM1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : 4 µg/mL of Raji cell extract, 3 µg/mL of HepG2 cell extract, and 200 µg/mL of HeLa cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 5.71 pg/mL

Range

39.063 - 2500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human ICAM1 - Intracellular SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ICAM1 - Intracellular protein in human cell lysates. Quantitate Human ICAM1 - Intracellular with 5.71 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.2" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "91 - 107 %", "average": "= 100" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309183-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB309183-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB309183-CMP10", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB309183-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB309183-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB309183-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human ICAM1 - Intracellular Capture Antibody", "number":"AB309183-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human ICAM1 - Intracellular Lyophilized Recombinant Protein", "number":"AB309183-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB309183-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB309183-CMP12", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB309183-CMP11", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB309183-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human ICAM1 - Intracellular Detector Antibody", "number":"AB309183-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation.. (Microbial infection) Acts as a receptor for major receptor group rhinovirus A-B capsid proteins.. (Microbial infection) Acts as a receptor for Coxsackievirus A21 capsid proteins.. (Microbial infection) Upon Kaposi's sarcoma-associated herpesvirus/HHV-8 infection, is degraded by viral E3 ubiquitin ligase MIR2, presumably to prevent lysis of infected cells by cytotoxic T-lymphocytes and NK cell.
See full target information ICAM1
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websiteProtocolBooklet
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