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AB46080

Human ICAM2 ELISA Kit (CD102)

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Human ICAM2 ELISA Kit (CD102) is a Sandwich (quantitative) ELISA for the measurement of Human ICAM2 (CD102) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD102, Intercellular adhesion molecule 2, ICAM-2, ICAM2

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Sandwich ELISA - Human ICAM2 ELISA Kit (CD102) (AB46080)
  • sELISA

Supplier Data

Sandwich ELISA - Human ICAM2 ELISA Kit (CD102) (AB46080)

Representative Standard Curve using ab46080

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.2 U/mL

Range

0.75 - 24 U/mL

Assay time

1h 45m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Human ICAM2 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of soluble Inter Cellular Adhesion Molecule -2 (ICAM2) in Human serum, plasma, buffered solutions or cell culture medium.

A monoclonal antibody specific for ICAM2 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known ICAM2 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for ICAM2 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of ICAM2 present in the samples.

This kit will recognize both endogenous and recombinant Human ICAM2.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample B", "replicates": 33, "mean": "3120 U/µL", "standardDeviation": "0.218", "coefficientOfVariability": "6.98" } ]
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What's included?

{ "values": { "2x96Tests": { "sellingSize": "2 x 96 Tests", "publicAssetCode":"ab46080-2x96Tests", "assetComponentDetails": [ { "size":"1 x 23 mL", "name":"HRP Diluent", "number":"AB46080-CMP03" }, { "size":"2 x 1 Unit", "name":"ICAM2 Microplate (12 x 8 well strips)", "number":"AB46080-CMP10" }, { "size":"4 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46080-CMP05" }, { "size":"1 x 24 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46080-CMP08" }, { "size":"4 x 1 Vial", "name":"Control", "number":"AB46080-CMP09" }, { "size":"2 x 400 µL", "name":"Biotinylated anti-ICAM2", "number":"AB46080-CMP04" }, { "size":"2 x 10 mL", "name":"200X Wash Buffer", "number":"AB46080-CMP06" }, { "size":"1 x 25 mL", "name":"10X Standard Diluent Buffer", "number":"AB46080-CMP07" }, { "size":"2 x 11 mL", "name":"Stop Reagent", "number":"AB46080-CMP02" }, { "size":"1 x 13 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46080-CMP01" }, { "size":"4 x 1 Vial", "name":"ICAM2 Standard (Lyophilized)", "number":"AB46080-CMP11" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab46080-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"HRP Diluent", "number":"AB46080-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"10X Standard Diluent Buffer", "number":"AB46080-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"ICAM2 Standard (Lyophilized)", "number":"AB46080-CMP11", "productcode":"" }, { "size":"1 x 10 mL", "name":"200X Wash Buffer", "number":"AB46080-CMP06", "productcode":"" }, { "size":"1 x 400 µL", "name":"Biotinylated anti-ICAM2", "number":"AB46080-CMP04", "productcode":"" }, { "size":"2 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46080-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"ICAM2 Microplate (12 x 8 well strips)", "number":"AB46080-CMP10", "productcode":"" }, { "size":"1 x 7.5 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46080-CMP01", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Reagent", "number":"AB46080-CMP02", "productcode":"" }, { "size":"1 x 11 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46080-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Control", "number":"AB46080-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ICAM2 also known as IC2 or ICAM-2 is a cell surface glycoprotein with a molecular mass of approximately 55-65 kDa. It plays an important role in immune response. The protein belongs to the immunoglobulin superfamily and interacts specifically with integrin molecules. ICAM2 is expressed widely across many tissues including endothelial cells platelets and leukocytes. Its primary function involves facilitating stable adhesion between leukocytes and endothelial cells which is essential for leukocyte transmigration.
Biological function summary

ICAM2 helps in immune function by serving as an adhesion molecule. It does not form part of a larger protein complex but instead it acts independently to mediate interactions between cells. It contributes to leukocyte rolling and firm adhesion stages necessary for leukocyte extravasation. This function is important in immune surveillance and response to infection allowing immune cells to quickly migrate to sites of inflammation.

Pathways

ICAM2 operates in the leukocyte adhesion and transendothelial migration pathways. It interacts closely with integrins especially LFA-1 present on leukocytes and this interaction supports cell movement during immune responses. It also participates in signaling pathways that activate immune cells further aiding in the regulation of immune cell activity. These interactions make ICAM2 a critical component in immunological communication and signal transduction pathways.

ICAM2 is associated with conditions involving immune dysfunction and inflammation. For instance abnormal expression of ICAM2 has been linked to inflammatory diseases such as atherosclerosis. Additionally it plays a role in the pathology of multiple sclerosis where it might influence the migration of immune cells across the blood-brain barrier. Its relationship with integrins highlights its potential relevance in therapeutic strategies to modulate immune responses in these diseases.
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Product protocols

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Target data

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.
See full target information ICAM2
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websiteProtocolBooklet
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