Human ICAM3 ELISA Kit (CD50)
- Recombinant
- SimpleStep
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(1 Publication)
Human ICAM3 ELISA Kit (CD50) is a single-wash 90-min Simplestep used to quantify Human ICAM3 (CD50) with a sensitivity of 0.036 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
CD50, Intercellular adhesion molecule 3, ICAM-3, CDw50, ICAM-R, ICAM3
- sELISA
Supplier Data
Sandwich ELISA - Human ICAM3 ELISA Kit (CD50) (AB275097)
Interpolated concentrations of native ICAM3 in human serum, plasma (EDTA), plasma (citrate), and plasma (heparin).
The concentrations of ICAM3 were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 12.5%, plasma (EDTA) 12.5%, plasma (citrate) 12.5%, and plasma (heparin) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean target concentration was determined to be 124.4 ng/mL in serum, 87.3 ng/mL in plasma (EDTA), 96.7 ng/mL in and plasma (citrate), 105 ng/mL in plasma (heparin).
- sELISA
Supplier Data
Sandwich ELISA - Human ICAM3 ELISA Kit (CD50) (AB275097)
Serum from eight individual healthy ICAM3 female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ICAM3 concentration was determined to be 87.3 ng/mL with a range of 51 – 128 ng/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human ICAM3 ELISA Kit (CD50) (AB275097)
Example of human ICAM3 standard curve in Sample Diluent NS.
The ICAM3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Reactivity data
Product details
Human ICAM3 ELISA kit (ab275097) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human ICAM3 protein in serum, plasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human ICAM3 with 0.036 ng/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Intracellular adhesion molecule 3 (ICAM-3), also known as CD50, is a protein encoded by the gene ICAM3. ICAM3 belongs to the ICAM family of proteins, where all ICAM proteins are type I transmembrane glycoproteins, contain several immunoglobulin-like c2 domains, and bind to leukocyte adhesion protein (LFA-1). In addition, ICAM-3 is a ligand for integrin alpha-D/beta-2, where it contributes to apoptotic neutrophil phagocytosis by macrophages.
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Properties and storage information
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Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ICAM-3 contributes to the immune system’s functionality by mediating intercellular adhesion and cellular signaling. It acts as a ligand for the integrin LFA-1 found on leukocytes which facilitates the initial immune response. ICAM-3 is not a part of a larger complex but works closely with LFA-1 in homing and recruitment of immune cells to inflammation sites. Its interaction with LFA-1 also supports the formation of the immunological synapse an important step in T-cell activation and immune signaling.
Pathways
ICAM-3 contributes to both the immune response and leukocyte extravasation pathways. The interaction with LFA-1 places it within the context of immune cell signaling pathways impacting integrin-mediated signaling. Another pathway involving ICAM-3 is the cell adhesion pathway which is essential for maintaining proper immune surveillance and response. It collaborates with proteins like ICAM-1 in these pathways to ensure effective immune activity.
Product protocols
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Publications (1)
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Scientific reports 14:11535 PubMed38773159
2024
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