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AB46024

Human ICAM3 ELISA Kit (CD50)

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Human ICAM3 ELISA Kit (CD50) is a Sandwich (quantitative) ELISA for the measurement of Human ICAM3 (CD50) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD50, Intercellular adhesion molecule 3, ICAM-3, CDw50, ICAM-R, ICAM3

1 Images
Sandwich ELISA - Human ICAM3 ELISA Kit (CD50) (AB46024)
  • sELISA

Supplier Data

Sandwich ELISA - Human ICAM3 ELISA Kit (CD50) (AB46024)

Representative Standard Curve using ab46024

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 0.3 ng/mL

Range

0.62 - 20 ng/mL

Assay time

1h 45m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's ICAM3 (CD50) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of ICAM3 in serum, plasma, buffered solutions and cell culture medium.

A monoclonal antibody specific for ICAM3 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known ICAM3 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for ICAM3 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of ICAM3 present in the samples.

This kit will recognize both endogenous and recombinant Human ICAM3.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample A", "replicates": 36, "mean": "19.51 ng/mL", "standardDeviation": "0.391", "coefficientOfVariability": "1.99" }, { "reproducibilityType": "Inter", "sample": "Sample B", "replicates": 36, "mean": "2.71 ng/mL", "standardDeviation": "0.176", "coefficientOfVariability": "6.27" }, { "reproducibilityType": "Intra", "sample": "Sample A", "replicates": 8, "mean": "19.75 ng/mL", "standardDeviation": "0.172", "coefficientOfVariability": "0.86" }, { "reproducibilityType": "Intra", "sample": "Sample B", "replicates": 8, "mean": "2.83 ng/mL", "standardDeviation": "0.099", "coefficientOfVariability": "3.49" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ICAM-3 also known as CD50 or Intercellular Adhesion Molecule 3 is a cell surface glycoprotein. It weighs approximately 120 kilodaltons. ICAM-3 is expressed majorly on leukocytes including lymphocytes monocytes and granulocytes. It is a member of the immunoglobulin superfamily that participates in cell-cell interaction particularly in immune response. By engaging with integrin receptors mainly LFA-1 ICAM-3 plays a role in leukocyte adhesion and signaling.
Biological function summary

ICAM-3 contributes to the immune system’s functionality by mediating intercellular adhesion and cellular signaling. It acts as a ligand for the integrin LFA-1 found on leukocytes which facilitates the initial immune response. ICAM-3 is not a part of a larger complex but works closely with LFA-1 in homing and recruitment of immune cells to inflammation sites. Its interaction with LFA-1 also supports the formation of the immunological synapse an important step in T-cell activation and immune signaling.

Pathways

ICAM-3 contributes to both the immune response and leukocyte extravasation pathways. The interaction with LFA-1 places it within the context of immune cell signaling pathways impacting integrin-mediated signaling. Another pathway involving ICAM-3 is the cell adhesion pathway which is essential for maintaining proper immune surveillance and response. It collaborates with proteins like ICAM-1 in these pathways to ensure effective immune activity.

ICAM-3 is associated with conditions such as inflammatory diseases and certain autoimmune disorders. Its expression levels and binding interactions can affect the severity and progression of such diseases. In autoimmune diseases ICAM-3 interaction with LFA-1 might contribute to the aberrant immune activation observed. Additionally studies have highlighted its role in infections where viruses exploit ICAM proteins to enter or spread showing its importance in pathogenesis-related conditions.
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Product protocols

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Target data

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2) (PubMed : 1448173). ICAM3 is also a ligand for integrin alpha-D/beta-2. In association with integrin alpha-L/beta-2, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed : 23775590).
See full target information ICAM3
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websiteProtocolBooklet
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