Human ICAM5 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human ICAM5 with a sensitivity of 16 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Cell culture supernatant, Cerebral Spinal Fluid
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 70.313 - 4500 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 16 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2).
Alternative names
TLCN, TLN, ICAM5, Intercellular adhesion molecule 5, ICAM-5, Telencephalin
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Human ICAM5 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human ICAM5 with a sensitivity of 16 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Cell culture supernatant, Cerebral Spinal Fluid
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 70.313 - 4500 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 16 pg/mL
- General recovery
- 98%
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. 12.1 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 9.3 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 98 | Range 92 - 101 % |
Sample type Citrate plasma | Average % = 105 | Range 99 - 114 % |
Sample type EDTA Plasma | Average % = 98 | Range 94 - 105 % |
Sample type Heparin Plasma | Average % = 104 | Range 97 - 109 % |
Sample type Cell culture supernatant | Average % = 97 | Range 94 - 101 % |
Sample type Cerebral Spinal Fluid | Average % = 105 | Range 104 - 107 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human ICAM5 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ICAM5 protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture supernatant, Cerebral Spinal Fluid, Cell culture supernatant. Quantitate Human ICAM5 with 16 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ICAM5 also known as Telencephalin is an intercellular adhesion molecule with a molecular mass of approximately 130 kDa. This protein is mainly expressed in the brain specifically in neurons of the telencephalon region. ICAM5 belongs to the immunoglobulin superfamily and plays a role in cell-cell adhesion. It does not demonstrate tight adherence like other ICAMs which suggests its specific function might differ from other ICAM family members.
- Biological function summary
ICAM5 participates in neuron-neuron adhesion processes. It plays a significant role in the development and maintenance of synaptic structures in the nervous system. ICAM5 does not form a complex with other proteins in its functioning. It contributes to the organization and stabilization of dendritic spines which are important for synaptic plasticity and neural communication. This involvement highlights the importance of ICAM5 in neural development and synaptic function.
- Pathways
ICAM5 integrates into signaling pathways related to neuronal development and synaptic plasticity. It is a part of the adhesion and cell recognition processes in neural systems. Through its involvement ICAM5 associates with pathways like the glutamatergic synapse pathway. Additionally ICAM5 shows a connection with proteins involved in membrane transport and neuronal signaling such as NMDARs (N-methyl-D-aspartate receptors) emphasizing its role in synaptic modulation.
- Associated diseases and disorders
ICAM5 relates to conditions affecting the nervous system such as schizophrenia and Alzheimer's disease. Studies indicate alterations in ICAM5 expression levels in patients with these diseases suggesting a potential impact on synaptic integrity and function. In Alzheimer's disease ICAM5 interacts with pathways involving beta-amyloid peptide accumulation indicating a possible connection with oxidative stress and neurodegenerative processes. In schizophrenia disruptions in ICAM5 associations with neural adhesion molecules could affect synaptic signaling and stability contributing to cognitive deficits.
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4 product images
Sandwich ELISA - Human ICAM5 ELISA Kit (ab314854)
Example of human ICAM5 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human ICAM5 ELISA Kit (ab314854)
Interpolated concentration of native ICAM5 was measured in duplicate at different sample concentrations. Undiluted samples are as follows: U-87 MG cell culture supernatant 50%, SH-SY5Y cell culture supernatant 100%, and CSF 12.5%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS. No detectable signal was observed in cell culture media.
Sandwich ELISA - Human ICAM5 ELISA Kit (ab314854)
Interpolated concentration of native ICAM5 was measured in duplicate at different sample concentrations. Undiluted samples are as follows: Serum 25%, plasma (citrate) 25%, plasma (EDTA) 50%, plasma (heparin) 25%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human ICAM5 ELISA Kit (ab314854)
Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ICAM5 concentration was determined to be 4,397 pg/mL with a range of 2,267 – 6,953 pg/mL.
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