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AB314854

Human ICAM5 ELISA Kit

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(1 Publication)

Human ICAM5 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human ICAM5 with a sensitivity of 16 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)

View Alternative Names

TLCN, TLN, ICAM5, Intercellular adhesion molecule 5, ICAM-5, Telencephalin

4 Images
Sandwich ELISA - Human ICAM5 ELISA Kit (AB314854)
  • sELISA

Supplier Data

Sandwich ELISA - Human ICAM5 ELISA Kit (AB314854)

Interpolated concentrations of human ICAM5 in serum, plasma (citrate), plasma (EDTA), and plasma (heparin)

Interpolated concentration of native ICAM5 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : Serum 25%, plasma (citrate) 25%, plasma (EDTA) 50%, plasma (heparin) 25%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human ICAM5 ELISA Kit (AB314854)
  • sELISA

Supplier Data

Sandwich ELISA - Human ICAM5 ELISA Kit (AB314854)

Interpolated concentrations of ICAM5 in normal human serum donors

Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ICAM5 concentration was determined to be 4,397 pg/mL with a range of 2,267 – 6,953 pg/mL.

Sandwich ELISA - Human ICAM5 ELISA Kit (AB314854)
  • sELISA

Supplier Data

Sandwich ELISA - Human ICAM5 ELISA Kit (AB314854)

Interpolated concentrations of human ICAM5 in cell culture supernatants (SN) and CSF

Interpolated concentration of native ICAM5 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : U-87 MG cell culture supernatant 50%, SH-SY5Y cell culture supernatant 100%, and CSF 12.5%.  The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS. No detectable signal was observed in cell culture media.

Sandwich ELISA - Human ICAM5 ELISA Kit (AB314854)
  • sELISA

Supplier Data

Sandwich ELISA - Human ICAM5 ELISA Kit (AB314854)

Example of human ICAM5 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Cell culture supernatant, Cerebral Spinal Fluid

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 16 pg/mL

Range

70.313 - 4500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Proteins & Peptides

AB276709

Recombinant Human ICAM5 protein (His tag)

SDS-PAGE - Recombinant Human ICAM5 protein (His tag) (AB276709)

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Primary Antibodies

AB302899

Anti-ICAM5 antibody [EPR25850-86]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry - Anti-ICAM5 antibody [EPR25850-86] (AB302899)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human ICAM5 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ICAM5 protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture supernatant, Cerebral Spinal Fluid, Cell culture supernatant. Quantitate Human ICAM5 with 16 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
        -High sensitivity, specificity and reproducibility from superior antibodies
        -Fully validated in biological samples
        -96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "12.1" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.3" } ]
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Recovery

[ { "sample": "Serum", "range": "92 - 101 %", "average": "= 98" }, { "sample": "Citrate plasma", "range": "99 - 114 %", "average": "= 105" }, { "sample": "EDTA Plasma", "range": "94 - 105 %", "average": "= 98" }, { "sample": "Heparin Plasma", "range": "97 - 109 %", "average": "= 104" }, { "sample": "Cell culture supernatant", "range": "94 - 101 %", "average": "= 97" }, { "sample": "Cerebral Spinal Fluid", "range": "104 - 107 %", "average": "= 105" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ICAM5 also known as Telencephalin is an intercellular adhesion molecule with a molecular mass of approximately 130 kDa. This protein is mainly expressed in the brain specifically in neurons of the telencephalon region. ICAM5 belongs to the immunoglobulin superfamily and plays a role in cell-cell adhesion. It does not demonstrate tight adherence like other ICAMs which suggests its specific function might differ from other ICAM family members.
Biological function summary

ICAM5 participates in neuron-neuron adhesion processes. It plays a significant role in the development and maintenance of synaptic structures in the nervous system. ICAM5 does not form a complex with other proteins in its functioning. It contributes to the organization and stabilization of dendritic spines which are important for synaptic plasticity and neural communication. This involvement highlights the importance of ICAM5 in neural development and synaptic function.

Pathways

ICAM5 integrates into signaling pathways related to neuronal development and synaptic plasticity. It is a part of the adhesion and cell recognition processes in neural systems. Through its involvement ICAM5 associates with pathways like the glutamatergic synapse pathway. Additionally ICAM5 shows a connection with proteins involved in membrane transport and neuronal signaling such as NMDARs (N-methyl-D-aspartate receptors) emphasizing its role in synaptic modulation.

ICAM5 relates to conditions affecting the nervous system such as schizophrenia and Alzheimer's disease. Studies indicate alterations in ICAM5 expression levels in patients with these diseases suggesting a potential impact on synaptic integrity and function. In Alzheimer's disease ICAM5 interacts with pathways involving beta-amyloid peptide accumulation indicating a possible connection with oxidative stress and neurodegenerative processes. In schizophrenia disruptions in ICAM5 associations with neural adhesion molecules could affect synaptic signaling and stability contributing to cognitive deficits.
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Product protocols

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Target data

Cell adhesion molecule that functions as a receptor ligand of the signaling receptor ITGAL : ITGB2/LFA-1 (lymphocyte-function associated (LFA) molecule 1) ensuring neuron cell-leukocyte adhesion (PubMed : 8995416). Creates homophilic cell adhesion promoting dendritogenesis and arborization of hippocampal neurons (PubMed : 10893271).
See full target information ICAM5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

CNS neuroscience & therapeutics 30:e70173 PubMed39727329

2024

Annexin A1 Mitigates Blood-Brain Barrier Disruption in a Sepsis-Associated Encephalopathy Model by Enhancing the Expression of Occludin and Zonula Occludens-1 (ZO-1).

Applications

Unspecified application

Species

Unspecified reactive species

Yao Li,Fang Zhou,Jiyue You,Xinran Gong
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Product promise

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