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AB267638

Human ICOS ELISA Kit

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Human ICOS ELISA Kit is a Sandwich ELISA for the measurement of Human ICOS in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD278, AILIM, ICOS, Inducible T-cell costimulator, Activation-inducible lymphocyte immunomediatory molecule

1 Images
Sandwich ELISA - Human ICOS ELISA Kit (AB267638)
  • sELISA

Supplier Data

Sandwich ELISA - Human ICOS ELISA Kit (AB267638)

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.2 ng/mL

Range

1.229 - 300 ng/mL

Assay Platform

Microplate

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Complementary Products

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AB105227

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Proteins & Peptides

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Recombinant Mouse ICOS protein (His tag)

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Primary Antibodies

AB201825

Alexa Fluor® 647 Anti-PD1 antibody [EPR4877(2)]

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Primary Antibodies

AB237712

Anti-CTLA4 antibody [CAL49]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTLA4 antibody [CAL49] (AB237712)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human ICOS ELISA Kit is designed for the quantitative determination of ICOS in cell culture supernatants, plasma and serum samples.

This assay employs an antibody specific for human ICOS coated on a 96-well plate. Standards and samples are pipetted into the wells and ICOS present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human ICOS antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ICOS bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "76 - 94 %", "average": "= 84.1" }, { "sample": "Plasma", "range": "77 - 86 %", "average": "= 81.74" }, { "sample": "Cell culture media", "range": "97 - 106 %", "average": "= 102" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267638-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB267638-CMP06", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB267638-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human ICOS coated Microplate (12 x 8 wells)", "number":"AB267638-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB267638-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human ICOS Standard (Lyophilized)", "number":"AB267638-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human ICOS Detection Antibody", "number":"AB267638-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB267638-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB267638-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB267638-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Inducible T-cell costimulator (ICOS) also known as AILIM or CD278 is a member of the CD28 superfamily of proteins. ICOS is a surface receptor protein approximately 60 kDa in mass. This protein is expressed mainly on activated T cells. It does not appear on naive or resting T cells. ICOS plays an important mechanical role in the immune response as its engagement enhances the production of cytokines and supports cell-cell interactions through its binding to the ICOS ligand.
Biological function summary

ICOS plays a critical role in the regulation of immune responses. It acts as a costimulatory signal for T cells supporting their proliferation and differentiation. ICOS helps form part of a complex involving interactions with the ICOS ligand present on antigen-presenting cells. This interaction affects the function and fate of various T cell subsets including T follicular helper cells which are important for antibody production.

Pathways

ICOS is part of the immune signaling pathway. It associates closely with the CD28 signaling pathway enhancing T-cell activation and survival. ICOS interacts with proteins such as the ICOS ligand (ICOSL) on antigen-presenting cells which helps mediate its effects. These pathways are essential in immune system homeostasis and adaptive immune responses where dysfunction can lead to immune-related diseases.

ICOS has implications in conditions such as autoimmune diseases and cancer. Abnormal expression or function of ICOS relates to the development of autoimmune conditions like systemic lupus erythematosus (SLE) where the overactive immune response damages the body's tissues. Additionally ICOS might support tumor progression in certain types of cancers by promoting immune escape. It interacts with regulatory proteins like CTLA-4 and PD-1 in these diseases which are notably involved in immune checkpoint pathways.
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Product protocols

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Target data

Stimulatory receptor expressed in activated or antigen-experienced T-cells that plays an important role in the immune response (PubMed : 9930702). Upon binding to its ligand ICOSL expressed on antigen presenting cells (APCs), delivers costimulatory signals that enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines including IL10, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells (PubMed : 33033255). Also acts as a costimulatory receptor critical for the differentiation of T follicular regulatory cells upon immune challenges such as viral infection (PubMed : 27135603). Mechanistically, potentiates TCR-induced calcium flux by augmenting PLCG1 activation and actin remodeling (By similarity). In addition, activates PI3K signaling pathways independently of calcium flux (PubMed : 30523347). Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes (By similarity).
See full target information ICOS
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websiteProtocolBooklet
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Product promise

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