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AB245710

Human IDO ELISA Kit

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(4 Publications)

Human IDO ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IDO with a sensitivity of 13 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

IDO, INDO, IDO1, IDO-1

6 Images
ELISA - Human IDO ELISA Kit (AB245710)
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Supplier Data

ELISA - Human IDO ELISA Kit (AB245710)
Sandwich ELISA - Human IDO ELISA Kit (AB245710)
  • sELISA

Supplier Data

Sandwich ELISA - Human IDO ELISA Kit (AB245710)

Interpolated concentrations of native IDO in IFN stimulated HeLa cell extracts based on a 20 μg/mL extract load.

The concentrations of IDO were measured in duplicate and interpolated from the IDO standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IDO concentration was determined to be 9033 pg/mL. IDO was not detected in naive HeLa cells with a 20 µg/mL extract load.

Sandwich ELISA - Human IDO ELISA Kit (AB245710)
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Lab

Sandwich ELISA - Human IDO ELISA Kit (AB245710)

Interpolated concentration of native IDO was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 10 µg/mL IFN-gamma stimulated HeLa extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human IDO ELISA Kit (AB245710)
  • sELISA

Supplier Data

Sandwich ELISA - Human IDO ELISA Kit (AB245710)

Representative image of Human IDO ELISA Kit (ab245710)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human IDO Capture Antibody

- 10X Human IDO Detector Antibody

- Human IDO Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody diluent 5BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Sandwich ELISA - Human IDO ELISA Kit (AB245710)
  • sELISA

Lab

Sandwich ELISA - Human IDO ELISA Kit (AB245710)

Example of human IDO standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IDO ELISA Kit (AB245710)
  • sELISA

Supplier Data

Sandwich ELISA - Human IDO ELISA Kit (AB245710)

Example of human IDO standard curve in 1X Cell Extraction Buffer PTR. The IDO standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 13 pg/mL

Range

78.125 - 5000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human IDO ELISA Kit ab245710 is a rapid single-wash 90-min Sandwich ELISA to measure Human IDO in cell culture extracts. This SimpleStep sensitivity is 13 pg/mL.

How the assay works

Human IDO SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human IDO ELISA Kit ab245710 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our 384-well Human IDO ELISA Kit only requires a maximum of 12.5µL sample

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Indoleamine 2, 3-dioxygenase antibody [EPR20374] - BSA and Azide free (Capture) ab252645
- Anti-Indoleamine 2, 3-dioxygenase antibody [EPR20374-97] - BSA and Azide free (Detector) ab252645

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Cell Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.6" }, { "reproducibilityType": "Intra", "sample": "Cell Extract", "replicates": 21, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3" } ]
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Recovery

[ { "sample": "Cell culture extracts", "range": "81 - 100 %", "average": "= 93" } ]
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What's included?

{ "values": { "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab245710-1x384Tests", "assetComponentDetails": [ { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB245710-CMP13" }, { "size":"2 x 1 Vial", "name":"Human IDO Lyophilized Recombinant Protein", "number":"AB245710-CMP12" }, { "size":"1 x 600 µL", "name":"10X Human IDO Capture Antibody", "number":"AB245710-CMP10" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB245710-CMP09" }, { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB245710-CMP04" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB245710-CMP08" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB245710-CMP05" }, { "size":"1 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB245710-CMP07" }, { "size":"6 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB245710-CMP06" }, { "size":"1 x 600 µL", "name":"10X Human IDO Detector Antibody", "number":"AB245710-CMP11" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB245710-CMP15" } ] }, "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab245710-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB245710-CMP04" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB245710-CMP13" }, { "size":"10 x 2 Vial", "name":"Human IDO Lyophilized Recombinant Protein", "number":"AB245710-CMP12" }, { "size":"10 x 600 µL", "name":"10X Human IDO Capture Antibody", "number":"AB245710-CMP10" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB245710-CMP09" }, { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB245710-CMP08" }, { "size":"10 x 600 µL", "name":"10X Human IDO Detector Antibody", "number":"AB245710-CMP11" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB245710-CMP03" }, { "size":"10 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB245710-CMP06" }, { "size":"2 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB245710-CMP07" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB245710-CMP05" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab245710-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB245710-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB245710-CMP09", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB245710-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB245710-CMP14", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB245710-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB245710-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB245710-CMP13", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IDO Lyophilized Recombinant Protein", "number":"AB245710-CMP12", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IDO Capture Antibody", "number":"AB245710-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB245710-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IDO Detector Antibody", "number":"AB245710-CMP11", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB245710-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Indoleamine 2 3-dioxygenase (IDO) also known as IDO1 is an enzyme involved in the catabolism of tryptophan to kynurenine. It weighs approximately 45 kDa and it is an important target in immunoregulatory processes. IDO is highly expressed in antigen-presenting cells such as dendritic cells and macrophages as well as in various tumor cells where it helps modulate immune responses. This enzyme can also be measured by using assays like IDO ELISA.
Biological function summary

IDO plays a role in immune response regulation by degrading tryptophan an essential amino acid needed for T-cell proliferation. The depletion of tryptophan and the accumulation of its metabolites such as kynurenine cause immunosuppressive effects within the tissue microenvironment. While IDO functions mostly as a standalone enzyme its activity influences the cellular surroundings by altering the balance of local immune reactions.

Pathways

IDO integrates into the tryptophan metabolism pathway and is important in the kynurenine pathway. Through this pathway it maintains immune homeostasis and cell defense mechanisms. Proteins like kynureninase and kynurenine 3-monooxygenase also participate in the same metabolic processes which further extend the effects of tryptophan metabolism on immune cell behavior.

IDO is linked with cancer and chronic inflammatory conditions. In cancer elevated IDO expression suppresses anti-tumor immunity aiding tumor cells to evade immune surveillance. It also plays a role in autoimmune disorders by regulating excessive immune activity. During cancer progression IDO often works in conjunction with immune checkpoint proteins like PD-L1 which further enhances its immunosuppressive capabilities within the tumor microenvironment.
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Product protocols

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Target data

Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway (PubMed : 17671174). Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses (PubMed : 25691885). Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells (PubMed : 25691885). Acts as a suppressor of anti-tumor immunity (PubMed : 14502282, PubMed : 23103127, PubMed : 25157255, PubMed : 25691885). Limits the growth of intracellular pathogens by depriving tryptophan (PubMed : 25691885). Protects the fetus from maternal immune rejection (PubMed : 25691885).
See full target information IDO1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:463 PubMed40541951

2025

ARNTL-mediated INO80-DHX15 axis reprograms the glycolytic metabolism and augments the progression of endometrial carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Ye,Genyi Jiang,Yihan Sun,Bilan Li

Cell reports. Medicine 5:101717 PubMed39243754

2024

mRNA-delivery of IDO1 suppresses T cell-mediated autoimmunity.

Applications

Unspecified application

Species

Unspecified reactive species

Laurie L Kenney,Rebecca Suet-Yan Chiu,Michelle N Dutra,Alexandra Wactor,Chris Honan,Lukas Shelerud,Joshua J Corrigan,Kelly Yu,Joseph D Ferrari,Kate L Jeffrey,Eric Huang,Paul L Stein

Diabetes 70:1561-1574 PubMed33858824

2021

Diabetic Kidney Disease Alters the Transcriptome and Function of Human Adipose-Derived Mesenchymal Stromal Cells but Maintains Immunomodulatory and Paracrine Activities Important for Renal Repair.

Applications

Unspecified application

Species

Unspecified reactive species

LaTonya J Hickson,Alfonso Eirin,Sabena M Conley,Timucin Taner,Xiaohui Bian,Ahmed Saad,Sandra M Herrmann,Ramila A Mehta,Travis J McKenzie,Todd A Kellogg,James L Kirkland,Tamar Tchkonia,Ishran M Saadiq,Hui Tang,Kyra L Jordan,Xiangyang Zhu,Mathew D Griffin,Andrew D Rule,Andre J van Wijnen,Stephen C Textor,Lilach O Lerman

Frontiers in immunology 11:602530 PubMed33584669

2021

Irradiated Tumor Fibroblasts Avoid Immune Recognition and Retain Immunosuppressive Functions Over Natural Killer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Nannan Yang,Kristin Lode,Rodrigo Berzaghi,Ashraful Islam,Inigo Martinez-Zubiaurre,Turid Hellevik
View all publications
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websiteProtocolBooklet
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