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AB313973

Human IDUA ELISA Kit

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Human IDUA ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human IDUA in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Alpha-L-iduronidase, IDUA

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Sandwich ELISA - Human IDUA ELISA Kit (AB313973)
  • sELISA

Supplier Data

Sandwich ELISA - Human IDUA ELISA Kit (AB313973)

Example of standard curve using ab313973. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.16 ng/mL

Range

0.16 - 40 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human IDUA ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IDUA in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human IDUA coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "82 - 120 %", "average": "= 101.5" }, { "sample": "Plasma", "range": "87 - 127 %", "average": "= 101.2" }, { "sample": "Cell culture media", "range": "68 - 108 %", "average": "= 90.34" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313973-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB313973-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human IDUA Protein Standard", "number":"AB313973-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human IDUA Detection Antibody", "number":"AB313973-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human IDUA Antibody-coated ELISA Plate", "number":"AB313973-CMP08", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313973-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"1000X HRP-Streptavidin", "number":"AB313973-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313973-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313973-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IDUA also known as alpha-L-iduronidase functions as an enzyme responsible for the breakdown of glycosaminoglycans. Its mechanical role involves the hydrolysis of the terminal α-L-iduronic acid residues essential in the degradation process of heparan sulfate and dermatan sulfate. The average molecular mass of IDUA is approximately 72 kDa. It expresses predominantly in lysosomes the cell's compartments that deal with digestion and waste processing. IDUA performs its functions most notably in tissues with high turnover of glycosaminoglycans such as liver spine and connective tissue.
Biological function summary

Alpha-L-iduronidase plays an important role in maintaining normal cellular operations by catalyzing important steps of the lysosomal degradation pathway. It is not part of a complex but functions independently within the lysosome. Through its enzymatic activity IDUA prevents the accumulation of undegraded molecules which could otherwise disrupt cellular function. The proper function of IDUA maintains cellular homeostasis illustrating its necessity for regular cellular maintenance.

Pathways

IDUA integrates most prominently into the lysosomal storage pathway due to its fundamental role in glycosaminoglycan degradation. This pathway includes other related enzymes like iduronate-2-sulfatase which work synergistically to ensure the complete breakdown of glycosaminoglycans. Another notable pathway where IDUA plays a part is the catabolism of carbohydrates linking it to various carbohydrate-active enzymes that collaboratively process complex sugar molecules.

Mutations or deficiencies in IDUA can result in significant health conditions such as Mucopolysaccharidosis type I (MPS I) which includes Hurler Hurler-Scheie and Scheie syndromes. These disorders arise from the accumulation of heparan sulfate and dermatan sulfate leading to a wide range of symptoms affecting multiple organs. IDUA's dysfunction connects to another enzyme iduronate-2-sulfatase which when also deficient could further exacerbate the accumulation of glycosaminoglycans and worsen clinical outcomes.
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Product protocols

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Target data

See full target information IDUA
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websiteProtocolBooklet
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Complementary Products

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