AB322432

Human Iduronate 2 sulfatase/SIDS ELISA Kit

Recombinant
SimpleStep
KO Validated
What is this?

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Human Iduronate 2 sulfatase/SIDS ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Iduronate 2 sulfatase/SIDS with a sensitivity of 15.75 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- KO validated for guaranteed specificity

View Alternative Names

SIDS, IDS, Iduronate 2-sulfatase, Alpha-L-iduronate sulfate sulfatase, Idursulfase

5 Images

Sandwich ELISA - Human Iduronate 2 sulfatase/SIDS ELISA Kit (AB322432)

sELISA

Supplier Data

Sandwich ELISA - Human Iduronate 2 sulfatase/SIDS ELISA Kit (AB322432)

Interpolated concentration of native Iduronate 2 sulfatase was measured in duplicate at different sample concentrations. Undiluted samples are as follows : A549 cell extract 200 µg/mL, HeLa cell extract 400 µg/mL, MCF-7 cell extract 200 µg/mL, and lung tissue extract 500 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Human Iduronate 2 sulfatase/SIDS ELISA Kit (AB322432)

Interpolated concentration of native Iduronate 2 sulfatase was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 400 µg/mL HeLa cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Human Iduronate 2 sulfatase/SIDS ELISA Kit (AB322432)

The concentration of Iduronate 2 sulfatase was measured in wild type and knockout HeLa cell extracts based on a 250 µg/mL extract load. The mean concentration was determined to be 1,215 pg/mL in wild type extract and undetectable in knockout extract. The control and Iduronate 2 sulfatase knockout HeLa cell lines are available under ab264747.

sELISA

Supplier Data

Sandwich ELISA - Human Iduronate 2 sulfatase/SIDS ELISA Kit (AB322432)

Example of human Iduronate 2 sulfatase standard curve. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Iduronate 2 sulfatase/SIDS ELISA Kit (AB322432)

Example of human Iduronate 2 sulfatase standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Tissue Extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 15.75 pg/mL

Range

65.625 - 4200 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Iduronate 2 sulfatase/SIDS SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Iduronate 2 sulfatase/SIDS protein in Cell Lysate and Tissue extracts. Quantitate Human Iduronate 2 sulfatase/SIDS with 15.75 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.4" } ]

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Recovery

[ { "sample": "Cell Lysate", "range": "89 - 100 %", "average": "= 94" }, { "sample": "Tissue Extracts", "range": "85 - 104 %", "average": "= 94" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Lysosomal enzyme involved in the degradation pathway of dermatan sulfate and heparan sulfate.

See full target information IDS

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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