Human Iduronate 2 sulfatase/SIDS ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Iduronate 2 sulfatase/SIDS with a sensitivity of 15.75 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- KO validated for guaranteed specificity
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 65.625 - 4200 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 15.75 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Lysosomal enzyme involved in the degradation pathway of dermatan sulfate and heparan sulfate.
Alternative names
SIDS, IDS, Iduronate 2-sulfatase, Alpha-L-iduronate sulfate sulfatase, Idursulfase
What's included?
Human Iduronate 2 sulfatase/SIDS ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Iduronate 2 sulfatase/SIDS with a sensitivity of 15.75 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- KO validated for guaranteed specificity
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 65.625 - 4200 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 15.75 pg/mL
- General recovery
- 94%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 8 | mean - | SD - | C.V. 9 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 3 | mean - | SD - | C.V. 3.4 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell Lysate | Average % = 94 | Range 89 - 100 % |
Sample type Tissue Extracts | Average % = 94 | Range 85 - 104 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Iduronate 2 sulfatase/SIDS SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Iduronate 2 sulfatase/SIDS protein in Cell Lysate and Tissue extracts. Quantitate Human Iduronate 2 sulfatase/SIDS with 15.75 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.
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5 product images
Sandwich ELISA - Human Iduronate 2 sulfatase/SIDS ELISA Kit (ab322432)
Example of human Iduronate 2 sulfatase standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Iduronate 2 sulfatase/SIDS ELISA Kit (ab322432)
Interpolated concentration of native Iduronate 2 sulfatase was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 400 µg/mL HeLa cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
Sandwich ELISA - Human Iduronate 2 sulfatase/SIDS ELISA Kit (ab322432)
Interpolated concentration of native Iduronate 2 sulfatase was measured in duplicate at different sample concentrations. Undiluted samples are as follows: A549 cell extract 200 µg/mL, HeLa cell extract 400 µg/mL, MCF-7 cell extract 200 µg/mL, and lung tissue extract 500 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
Sandwich ELISA - Human Iduronate 2 sulfatase/SIDS ELISA Kit (ab322432)
The concentration of Iduronate 2 sulfatase was measured in wild type and knockout HeLa cell extracts based on a 250 µg/mL extract load. The mean concentration was determined to be 1,215 pg/mL in wild type extract and undetectable in knockout extract. The control and Iduronate 2 sulfatase knockout HeLa cell lines are available under Human IDS (Iduronate 2 sulfatase/SIDS) knockout HeLa cell line ab264747.
Sandwich ELISA - Human Iduronate 2 sulfatase/SIDS ELISA Kit (ab322432)
Example of human Iduronate 2 sulfatase standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
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