Human IFN gamma (IFNG) ELISA kit is a single-wash, 90-minute SimpleStep ELISA® a used to quantify human interferon-gamma (IFN-γ) with a sensitivity of 470 pg/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step.
- Colorimetric sandwich ELISA - 450 nm readout; compatible with any standard plate reader
- Available in different formats to meet various throughput needs, including 384-well plates for higher throughput
- Broad sample compatibility – suitable for serum, plasma (citrate, EDTA), and cell culture supernatants
- Cited in over 25 publications
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- Science advances 9:eadj99642023Cellular immunity analysis by a modular acoustofluidic platform: CIAMAP.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuoyu Zhong,Matthew Sullivan,Neil Upreti,Roy Chen,Agustin De Ganzó,Kaichun Yang,Shujie Yang,Ke Jin,Ye He,Ke Li,Jianping Xia,Zhiteng Ma,Luke P Lee,Tania Konry,Tony Jun HuangPubMed 38134285
- Scientific reports 13:211802023Bromelain-loaded nanocomposites decrease inflammatory and cytotoxicity effects of gliadin on Caco-2 cells and peripheral blood mononuclear cells of celiac patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMasoumeh Sadat Mousavi Maleki,Ramin Ebrahimi Kiasari,Seyed Javad Seyed Mousavi,Hamid Hashemi-Moghaddam,Ali Akbar Shabani,Hamid Madanchi,Soroush SardariPubMed 38040898
- Nature communications 14:76392023Acoustofluidic Interfaces for the Mechanobiological Secretome of MSCs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYe He,Shujie Yang,Pengzhan Liu,Ke Li,Ke Jin,Ryan Becker,Jinxin Zhang,Chuanchuan Lin,Jianping Xia,Zhehan Ma,Zhiteng Ma,Ruoyu Zhong,Luke P Lee,Tony Jun HuangPubMed 37993431
- Clinical and translational medicine 13:e14102023Long noncoding RNA Regulating ImMune Escape regulates mixed lineage leukaemia protein-1-H3K4me3-mediated immune escape in oesophageal squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia Liu,Wei-Yi Zhou,Xiao-Jing Luo,Yan-Xing Chen,Chau-Wei Wong,Ze-Xian Liu,Jia- Bo Zheng,Hai- Yu Mo,Jun-Quan Chen,Jia-Jun Li,Ming Zhong,Yu-Hong Xu,Qi-Hua Zhang,Heng-Ying Pu,Qi-Nian Wu,Ying Jin,Zi-Xian Wang,Rui-Hua Xu,Hui-Yan LuoPubMed 37712124
- Biological & pharmaceutical bulletin 46:1260-12682023Cordycepin Enhances the Cytotoxicity of Human Natural Killer Cells against Cancerous Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNipha Chaicharoenaudomrung,Phongsakorn Kunhorm,Parinya NoisaPubMed 37661405
- Immunity, inflammation and disease 11:e8642023RNF182 induces p65 ubiquitination to affect PDL1 transcription and suppress immune evasion in lung adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXingdu Zeng,Xiaoyuan Tang,Xingxiang Chen,Huilan WenPubMed 37249301
- The Tohoku journal of experimental medicine 260:263-2712023Predictive Values of Blood Type I and Type II Interferon Production for Disease Activity and Clinical Response to TNF-α Blocking Therapy in Patients with Ankylosing Spondylitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYulan Hu,Bo Lou,Zhonghua Jiang,Chunchu YuPubMed 37081619
- Cell death discovery 9:922023Midkine inhibition enhances anti-PD-1 immunotherapy in sorafenib-treated hepatocellular carcinoma via preventing immunosuppressive MDSCs infiltration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLijuan Ding,Nanya Wang,Qiang Wang,Xia Fan,Yuning Xin,Shudong WangPubMed 36906597
- Medicine 102:e323842023Effects of amlodipine combined with atorvastatin on Th17/Treg imbalance and vascular microcirculation in hypertensive patients with atherosclerosis: A double-blind, single-center randomized controlled trial.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGui Yang,Youjiang QiuPubMed 36820569
- Cancers 15:2023Tumor Growth Suppression of Pancreatic Cancer Orthotopic Xenograft Model by CEA-Targeting CAR-T Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOsamu Sato,Takahiro Tsuchikawa,Takuma Kato,Yasunori Amaishi,Sachiko Okamoto,Junichi Mineno,Yuta Takeuchi,Katsunori Sasaki,Toru Nakamura,Kazufumi Umemoto,Tomohiro Suzuki,Linan Wang,Yizheng Wang,Kanako C Hatanaka,Tomoko Mitsuhashi,Yutaka Hatanaka,Hiroshi Shiku,Satoshi HiranoPubMed 36765558
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.468 - 30 ng/mL
- Assay time
- 1h 30m
- Sensitivity
- = 470 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation (PubMed:16914093, PubMed:8666937). Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation (PubMed:8349687). Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription (PubMed:16914093). Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits (PubMed:8666937). In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading (PubMed:8163024). Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference (PubMed:11112687). Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL (PubMed:7729559). Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation (By similarity).
Alternative names
Interferon gamma, IFN-gamma, Immune interferon, IFNG
What's included?
Recommended products
Human IFN gamma (IFNG) ELISA kit is a single-wash, 90-minute SimpleStep ELISA® a used to quantify human interferon-gamma (IFN-γ) with a sensitivity of 470 pg/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step.
- Colorimetric sandwich ELISA - 450 nm readout; compatible with any standard plate reader
- Available in different formats to meet various throughput needs, including 384-well plates for higher throughput
- Broad sample compatibility – suitable for serum, plasma (citrate, EDTA), and cell culture supernatants
- Cited in over 25 publications
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.468 - 30 ng/mL
- Assay time
- 1h 30m
- Assay Platform
- Microplate
- Sensitivity
- = 470 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 5 | C.V. 1.1 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 3 | C.V. 7.9 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 102 | Range 98 - 104 % |
Sample type Cell culture media | Average % = 86 | Range 77 - 93 % |
Sample type Citrate plasma | Average % = 107 | Range 96 - 115 % |
Sample type EDTA Plasma | Average % = 122 | Range 117 - 124 % |
Sample type Heparin Plasma | Average % = 244 | Range 197 - 298 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human IFN gamma ELISA Kit ab174443 is a rapid single-wash 90-min ELISA kit to measure Human IFN gamma in cell culture supernatant, serum and plasma. This SimpleStep ELISA® sensitivity is 470 pg/mL.
How the assay works
Human IFN gamma SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This IFN-γ ELISA Kit recognizes both native and recombinant human Interferon-gamma protein in serum, plasma, and cell culture supernatant samples only. Urine, milk, and cell and tissue extract samples have not been tested with this kit.
Human IFN gamma ELISA Kit ab174443 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Human IFN gamma ELISA Kit ab174443
Human IFN gamma ELISA Kit ab174443 has been used to study inflammation in different physio-pathological contexts such as celiac disease(1), pancreatic cancer(2)and atherosclerosis(3).
References:
(1)Soroush Sardari et al. 2023, PMID: 38040898,
(2)Satoshi Hirano et al. 2023, PMID: 36765558,
(3)Youjiang Qiu, et al. 2023, PMID: 36820569
Save your precious samples
Our 384-well Human IFN gamma ELISA Kit only requires a maxmum of 12.5µL
Related and recommended products
Human IFN gamma ELISA Kit ab174443 is commonly used to measure IFNg ELISA as a marker of inflammation.
Other ELISA kits to measure inflammatory markers include:
- Human IL-6 ELISA kit Human IL-6 ELISA Kit ab178013
- Human TNF alpha ELISA kit Human TNF alpha ELISA Kit ab181421
- Human IL-1 2 ELISA kit Human IL-2 ELISA Kit ab270883
To measure IFN gamma at a lower dynamic range (12.5-1600 pg/mL):
- Human IFN gamma High Sensitivity ELISA kit Human IFN gamma High Sensitivity ELISA Kit ab236895
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11 product images
ELISA - Human IFN gamma ELISA Kit (ab174443)
ELISA - Human IFN gamma ELISA Kit (ab174443)
Example IFNG standard curve for cell culture supernatant samples measurements. Background-subtracted data values (mean +/- SD) are graphed.
ELISA - Human IFN gamma ELISA Kit (ab174443)
Example IFNG standard curve for serum/plasma samples measurements. Background-subtracted data values (mean +/- SD) are graphed.
ELISA - Human IFN gamma ELISA Kit (ab174443)
Comparison of secreted IFNG in unstimulated and PHA-stimulated Human PBMC. PBMC were grown in the absence or presence of phytohemagglutinin (PHA) for 2 days. IFNG concentrations were measured in 12X and 6X diluted cell culture supernatants of the unstimulated PBMC and the stimulated PBMC, and media. Raw data values (mean +/-SD, n=3) are graphed. The dotted line represents zero sample background.
ELISA - Human IFN gamma ELISA Kit (ab174443)
Interpolated concentrations of secreted IFNG in unstimulated and PHA-stimulated Human PBMC. The concentrations of IFNG were interpolated from data values shown in Figure 3 using IFNG standard curve and corrected for sample dilution. The mean IFNG concentration was determined to be 1.8 ng/mL in unstimulated PBMC supernatants and 177.2 ng/mL in stimulated PBMC supernatants.
Sandwich ELISA - Human IFN gamma ELISA Kit (ab174443)
Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native Interferon-gamma was measured in PHA-stimulated PBMC cell culture supernatant samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
Recombinant Interferon-gamma was spiked into human serum and plasma (citrate and EDTA) samples and diluted in a 2-fold dilution series in Sample Diluent NBP.Neat pooled serum and plasma (EDTA and Citrate) samples from healthy donors was measured in duplicate. All values were below the detectable range of the assay.
Neat serum from ten individual healthy human female/male donors was measured in duplicate. All values were below the detectable range of the assay.
Sandwich ELISA - Human IFN gamma ELISA Kit (ab174443)
Example of human Interferon-gamma standard curve in Sample Diluent NBP (for serum/plasma samples).
The Interferon-gamma standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human IFN gamma ELISA Kit (ab174443)
Interpolated concentration of native IFN-gamma was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 20% treated human PBMC supernatant (5% PHA-M for 46hr). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human IFN gamma ELISA Kit (ab174443)
Example of human IFN-gamma standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human IFN gamma ELISA Kit (ab174443)
Example of human IFN-gamma standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human IFN gamma ELISA Kit (ab174443)
Example of human Interferon-gamma standard curve in Sample Diluent NS (for supernatant samples).
The Interferon-gamma standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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