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AB174443

Human IFN gamma ELISA Kit

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(37 Publications)

Human IFN gamma (IFNG) ELISA kit is a single-wash, 90-minute SimpleStep ELISA® a used to quantify human interferon-gamma (IFN-γ) with a sensitivity of 470 pg/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step.

- Colorimetric sandwich ELISA - 450 nm readout; compatible with any standard plate reader
- Available in different formats to meet various throughput needs, including 384-well plates for higher throughput
- Broad sample compatibility – suitable for serum, plasma (citrate, EDTA), and cell culture supernatants
- Cited in over 25 publications

View Alternative Names

Interferon gamma, IFN-gamma, Immune interferon, IFNG

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ELISA - Human IFN gamma ELISA Kit (AB174443)
  • ELISA

Supplier Data

ELISA - Human IFN gamma ELISA Kit (AB174443)
ELISA - Human IFN gamma ELISA Kit (AB174443)
  • ELISA

Supplier Data

ELISA - Human IFN gamma ELISA Kit (AB174443)

Interpolated concentrations of secreted IFNG in unstimulated and PHA-stimulated Human PBMC. The concentrations of IFNG were interpolated from data values using IFNG standard curve and corrected for sample dilution. The mean IFNG concentration was determined to be 1.8 ng/mL in unstimulated PBMC supernatants and 177.2 ng/mL in stimulated PBMC supernatants.

ELISA - Human IFN gamma ELISA Kit (AB174443)
  • ELISA

Supplier Data

ELISA - Human IFN gamma ELISA Kit (AB174443)

Comparison of secreted IFNG in unstimulated and PHA-stimulated Human PBMC. PBMC were grown in the absence or presence of phytohemagglutinin (PHA) for 2 days. IFNG concentrations were measured in 12X and 6X diluted cell culture supernatants of the unstimulated PBMC and the stimulated PBMC, and media. Raw data values (mean +/-SD, n=3) are graphed. The dotted line represents zero sample background.

ELISA - Human IFN gamma ELISA Kit (AB174443)
  • ELISA

Supplier Data

ELISA - Human IFN gamma ELISA Kit (AB174443)

Example IFNG standard curve for cell culture supernatant samples measurements. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IFN gamma ELISA Kit (AB174443)
  • sELISA

Supplier Data

Sandwich ELISA - Human IFN gamma ELISA Kit (AB174443)

Interpolated concentration of native IFN-gamma was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 20% treated human PBMC supernatant (5% PHA-M for 46hr). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human IFN gamma ELISA Kit (AB174443)
  • sELISA

Supplier Data

Sandwich ELISA - Human IFN gamma ELISA Kit (AB174443)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Native Interferon-gamma was measured in PHA-stimulated PBMC cell culture supernatant samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
Recombinant Interferon-gamma was spiked into human serum and plasma (citrate and EDTA) samples and diluted in a 2-fold dilution series in Sample Diluent NBP.

Neat pooled serum and plasma (EDTA and Citrate) samples from healthy donors was measured in duplicate. All values were below the detectable range of the assay.

Neat serum from ten individual healthy human female/male donors was measured in duplicate. All values were below the detectable range of the assay.

ELISA - Human IFN gamma ELISA Kit (AB174443)
  • ELISA

Supplier Data

ELISA - Human IFN gamma ELISA Kit (AB174443)

Example IFNG standard curve for serum/plasma samples measurements. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IFN gamma ELISA Kit (AB174443)
  • sELISA

Supplier Data

Sandwich ELISA - Human IFN gamma ELISA Kit (AB174443)

Example of human IFN-gamma standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IFN gamma ELISA Kit (AB174443)
  • sELISA

Supplier Data

Sandwich ELISA - Human IFN gamma ELISA Kit (AB174443)

Example of human IFN-gamma standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IFN gamma ELISA Kit (AB174443)
  • sELISA

Supplier Data

Sandwich ELISA - Human IFN gamma ELISA Kit (AB174443)

Example of human Interferon-gamma standard curve in Sample Diluent NS (for supernatant samples).

The Interferon-gamma standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IFN gamma ELISA Kit (AB174443)
  • sELISA

Supplier Data

Sandwich ELISA - Human IFN gamma ELISA Kit (AB174443)

Example of human Interferon-gamma standard curve in Sample Diluent NBP (for serum/plasma samples).

The Interferon-gamma standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 470 pg/mL

Range

0.468 - 30 ng/mL

Assay time

1h 30m

Assay Platform

Microplate

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Reactivity data

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Product details

Human IFN gamma ELISA Kit ab174443 is a rapid single-wash 90-min ELISA kit to measure Human IFN gamma in cell culture supernatant, serum and plasma. This SimpleStep ELISA® sensitivity is 470 pg/mL.

How the assay works

Human IFN gamma SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

This IFN-γ ELISA Kit recognizes both native and recombinant human Interferon-gamma protein in serum, plasma, and cell culture supernatant samples only. Urine, milk, and cell and tissue extract samples have not been tested with this kit.

Human IFN gamma ELISA Kit ab174443 protocol summary:

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Human IFN gamma ELISA Kit ab174443

Human IFN gamma ELISA Kit ab174443 has been used to study inflammation in different physio-pathological contexts such as celiac disease(1), pancreatic cancer(2)and atherosclerosis(3).

References:

(1)Soroush Sardari et al. 2023, PMID: 38040898,
(2)Satoshi Hirano et al. 2023, PMID: 36765558,
(3)Youjiang Qiu, et al. 2023, PMID: 36820569

Save your precious samples

Our 384-well Human IFN gamma ELISA Kit only requires a maxmum of 12.5µL

Related and recommended products

Human IFN gamma ELISA Kit ab174443 is commonly used to measure IFNg ELISA as a marker of inflammation.

Other ELISA kits to measure inflammatory markers include:

- Human IL-6 ELISA kit ab178013
- Human TNF alpha ELISA kit ab181421
- Human IL-1 2 ELISA kit ab270883

To measure IFN gamma at a lower dynamic range (12.5-1600 pg/mL):

- Human IFN gamma High Sensitivity ELISA kit ab236895

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.9" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.1" } ]
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Recovery

[ { "sample": "Serum", "range": "98 - 104 %", "average": "= 102" }, { "sample": "Cell culture media", "range": "77 - 93 %", "average": "= 86" }, { "sample": "Citrate plasma", "range": "96 - 115 %", "average": "= 107" }, { "sample": "EDTA Plasma", "range": "117 - 124 %", "average": "= 122" }, { "sample": "Heparin Plasma", "range": "197 - 298 %", "average": "= 244" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Interferon gamma (IFN-γ) also known as type II interferon is a cytokine that plays an important role in immune response. IFN-γ has a molecular weight of about 17 kDa and is produced by T cells and natural killer (NK) cells. IFN-γ binds to the interferon gamma receptor initiating a signaling cascade that activates various genes involved in immune functions. It is expressed mainly in activated immune cells within lymphoid tissues and inflamed sites during immune responses.
Biological function summary

This cytokine is significant in promoting macrophage activation enhancing the antigen presentation process and boosting the antimicrobial activity of phagocytes. IFN-γ is not part of a larger protein complex but works as a homodimer in signal transduction. Its production heightens the Th1 immune response by stimulating the differentiation of naпve T cells into Th1 cells which is essential for effective cellular immunity.

Pathways

IFN-γ is integrally involved in the JAK-STAT signaling pathway alongside another critical cytokine Interleukin-12. This pathway further amplifies the immune response by regulating the expression of genes associated with cellular defense mechanisms. IFN-γ also interacts with the NF-kB pathway influencing inflammation and the activation of further immune responses. These interactions show a network of cooperativity with proteins like STAT1 and NF-kB essential for executing its biological roles.

IFN-γ is linked to autoimmune diseases such as rheumatoid arthritis and multiple sclerosis where its elevated levels can exacerbate inflammatory processes. It connects to other proteins like TNF-alpha in promoting the inflammatory cascade. Moreover lower levels of IFN-γ are associated with a heightened risk of infections like tuberculosis demonstrating its vital role in pathogen defense. Therefore understanding IFN-γ and its interactions can be key in developing therapeutic approaches against these conditions.
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Product protocols

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Target data

Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation (PubMed : 16914093, PubMed : 8666937). Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation (PubMed : 8349687). Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription (PubMed : 16914093). Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits (PubMed : 8666937). In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading (PubMed : 8163024). Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference (PubMed : 11112687). Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL (PubMed : 7729559). Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation (By similarity).
See full target information IFNG
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Publications (37)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell communication and signaling 19:e12060 PubMed39720765

2024

M2-polarized tumor-associated macrophage-secreted exosomal lncRNA NEAT1 upregulates galectin-3 by recruiting KLF5 and promotes HCC immune escape.

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Species

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Wei Yuan,Qigang Sun,Xiaodan Zhu,Bo Li,Yongping Zou,Zhehao Liu

American journal of cancer research 14:4803-4816 PubMed39553202

2024

Predicting immunotherapy-related adverse events in late-stage non-small cell lung cancer with KARS G12C mutation treated with PD-1 inhibitors through combined assessment of LCP1 and ADPGK expression levels.

Applications

Unspecified application

Species

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Lei Chen,Cong Xu,Weihao Ren,Lei Yu,Tian Tang

Molecular carcinogenesis 64:162-175 PubMed39451071

2024

FOS-Mediated PLCB1 Induces Radioresistance and Weakens the Antitumor Effects of CD8 T Cells in Triple-Negative Breast Cancer.

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Species

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Yuxian Shu,Jun Lan,Huijing Luo,Huiying Fu,Xuhuang Xiao,Liping Yang

Discover oncology 15:562 PubMed39404969

2024

Expression of CLDN1 and EGFR in PTC.

Applications

Unspecified application

Species

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JunJie Wu,YouMei Wang,Lei Yan,YaWen Dong

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2406633 PubMed39116343

2024

PTBP3 Mediates IL-18 Exon Skipping to Promote Immune Escape in Gallbladder Cancer.

Applications

Unspecified application

Species

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Cheng Zhao,Jing-Wei Zhao,Yu-Han Zhang,Yi-di Zhu,Zi-Yi Yang,Shi-Lei Liu,Qiu-Yi Tang,Yue Yang,Hua-Kai Wang,Yi-Jun Shu,Ping Dong,Xiang-Song Wu,Wei Gong

Ophthalmic research 67:470-477 PubMed39097965

2024

Expressions of Type I-III Interferons in Sjögren's Syndrome and Non-Sjögren's Dry Eye.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Su,Lei Jiang,Yanpeng Wang,Yanping Dong,Yanjuan Yong,Kan Yang,Shixiong Gao

American journal of translational research 16:2670-2682 PubMed39006280

2024

Screening and analysis for potential clinical diagnostic and prognostic markers in allergic rhinitis.

Applications

Unspecified application

Species

Unspecified reactive species

Yejun Liu,Yonggang Kong,Xuhong Zhou

Folia biologica 70:53-61 PubMed38830123

2024

Gallic Acid Alleviates Psoriasis Keratinization and Inflammation by Regulating BRD4 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Li Zhang,Qiaoyuan Ye,Saiyang Gan,Huan Liu,Qing Zhang,Shuangshuang Wang,Can Cheng

Environmental toxicology 39:2980-2992 PubMed38317501

2024

Circ_0000877 accelerates proliferation and immune escape of non-small cell lung cancer cells by regulating microRNA-637/E2F2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Ting'an Chen,Zhengdong Li,Junzhu Chen,Zhe Xu

Oncology letters 27:106 PubMed38298427

2024

Microwave ablation enhances the systemic immune response in patients with lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Fuqi Ma,Yuhua Lin,Zhenhua Ni,Shiqiang Wang,Mengjie Zhang,Xiaoe Wang,Zhuhua Zhang,Xuming Luo,Xiayi Miao
View all publications
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