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AB46025

Human IFN gamma ELISA Kit

5

(1 Review)

|

(24 Publications)

Human IFN gamma ELISA Kit is a sandwich ELISA designed to quantify Human IFN gamma with a sensitivity of 5 pg/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 12.5 - 400 pg/mL
- Cited in over 20 publications

View Alternative Names

Interferon gamma, IFN-gamma, Immune interferon, IFNG

2 Images
Sandwich ELISA - Human IFN gamma ELISA Kit (AB46025)
  • sELISA

Unknown

Sandwich ELISA - Human IFN gamma ELISA Kit (AB46025)

Representative Standard Curve using ab46025

Sandwich ELISA - Human IFN gamma ELISA Kit (AB46025)
  • sELISA

Unknown

Sandwich ELISA - Human IFN gamma ELISA Kit (AB46025)

Jurkat were stimulated for 48 hours with 50 ng x mL-1 of PMA (ab120297) and 1 uM Ionomycin (ab120116) and PBMCs were stimulated for 48 hours with 2 % PHA-M (LifeTechnologies). Cell free supernatants were tested, showing results after background signal was subtracted (duplicates +/- SD).

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 5 pg/mL

Range

12.5 - 400 pg/mL

Assay time

2h 45m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human IFN gamma ELISA Kit ab46025 is a sandwich ELISA to measure Human IFN gamma in serum, plasma, cell culture supernatant with a sensitivity of 5 pg/ml.

How the assay works

A monoclonal antibody specific for IFN gamma has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IFN gamma concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IFN gamma are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IFN gamma present in the samples.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human IFN gamma ELISA Kit ab46025 protocol summary

1. Add standard or sample to each well used
2. Add prepared Biotinylated labeled detector antibody. Incubate at room temperatureAspirate and wash each well.
3. Add prepared Streptavidin-HRP mix to each well. Incubate at room temperature
4. Aspirate and wash each well.
5. Add the TMB Solution to each well until color develops and then add the Stop Solution.
6. Immediately begin recording the color development

Get results in 90 minutes with our SimpleStep ELISA® kit

Human IFN gamma ELISA Kit (ab174443)
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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample", "replicates": 30, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10" }, { "reproducibilityType": "Intra", "sample": "Sample", "replicates": 30, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.9" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Interferon gamma (IFN-γ) also known as type II interferon is a cytokine that plays an important role in immune response. IFN-γ has a molecular weight of about 17 kDa and is produced by T cells and natural killer (NK) cells. IFN-γ binds to the interferon gamma receptor initiating a signaling cascade that activates various genes involved in immune functions. It is expressed mainly in activated immune cells within lymphoid tissues and inflamed sites during immune responses.
Biological function summary

This cytokine is significant in promoting macrophage activation enhancing the antigen presentation process and boosting the antimicrobial activity of phagocytes. IFN-γ is not part of a larger protein complex but works as a homodimer in signal transduction. Its production heightens the Th1 immune response by stimulating the differentiation of naïve T cells into Th1 cells which is essential for effective cellular immunity.

Pathways

IFN-γ is integrally involved in the JAK-STAT signaling pathway alongside another critical cytokine Interleukin-12. This pathway further amplifies the immune response by regulating the expression of genes associated with cellular defense mechanisms. IFN-γ also interacts with the NF-kB pathway influencing inflammation and the activation of further immune responses. These interactions show a network of cooperativity with proteins like STAT1 and NF-kB essential for executing its biological roles.

IFN-γ is linked to autoimmune diseases such as rheumatoid arthritis and multiple sclerosis where its elevated levels can exacerbate inflammatory processes. It connects to other proteins like TNF-alpha in promoting the inflammatory cascade. Moreover lower levels of IFN-γ are associated with a heightened risk of infections like tuberculosis demonstrating its vital role in pathogen defense. Therefore understanding IFN-γ and its interactions can be key in developing therapeutic approaches against these conditions.
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Product protocols

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Target data

Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation (PubMed : 16914093, PubMed : 8666937). Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation (PubMed : 8349687). Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription (PubMed : 16914093). Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits (PubMed : 8666937). In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading (PubMed : 8163024). Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference (PubMed : 11112687). Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL (PubMed : 7729559). Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation (By similarity).
See full target information IFNG
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Publications (24)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:463 PubMed40541951

2025

ARNTL-mediated INO80-DHX15 axis reprograms the glycolytic metabolism and augments the progression of endometrial carcinoma.

Applications

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Species

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Lei Ye,Genyi Jiang,Yihan Sun,Bilan Li

Nutrients 17: PubMed39940325

2025

Immature Sword Bean () Pod Alleviates Allergic Rhinitis (A Double-Blind Trial) Through PI3K/Akt/mTOR Signaling.

Applications

Unspecified application

Species

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Hye-Jeong Hwang,Hyeock Yoon,Joo-Hyung Cho,Seong Lee,Kyung-A Hwang,Young Jun Kim

Molecular neurodegeneration 19:80 PubMed39456006

2024

Repetitive transcranial magnetic stimulation alleviates motor impairment in Parkinson's disease: association with peripheral inflammatory regulatory T-cells and SYT6.

Applications

Unspecified application

Species

Unspecified reactive species

Fen Xie,Bibiao Shen,Yuqi Luo,Hang Zhou,Zhenchao Xie,Shuzhen Zhu,Xiaobo Wei,Zihan Chang,Zhaohua Zhu,Changhai Ding,Kunlin Jin,Chengwu Yang,Lucia Batzu,K Ray Chaudhuri,Ling-Ling Chan,Eng-King Tan,Qing Wang

Journal of inflammation (London, England) 21:23 PubMed38907339

2024

Microcirculatory disturbance in acute liver injury is triggered by IFNγ-CD40 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Miho Kurokawa,Takeshi Goya,Motoyuki Kohjima,Masatake Tanaka,Sadahiro Iwabuchi,Shigeyuki Shichino,Satoshi Ueha,Tomonobu Hioki,Tomomi Aoyagi,Motoi Takahashi,Koji Imoto,Shigeki Tashiro,Hideo Suzuki,Masaki Kato,Shinichi Hashimoto,Hideo Matsuda,Kouji Matsushima,Yoshihiro Ogawa

iScience 27:109213 PubMed38439953

2024

CD169 classical monocyte as an important participant in Graves' ophthalmopathy through CXCL12-CXCR4 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Dongliang Wang,Jie Ling,RongQiang Tan,Huishi Wang,Yixin Qu,Xingyi Li,Jinshan Lin,Qikai Zhang,Qiuling Hu,Zhong Liu,Zhaojing Lu,Yuheng Lin,Li Sun,Dingqiao Wang,Ming Zhou,Zhuoxing Shi,Wuyou Gao,Huijing Ye,Xianchai Lin

African health sciences 23:239-245 PubMed38223593

2024

Associations of blood glucose, helper T cells and cytokine levels with degree of periodontal lesion in type 2 diabetes mellitus patients accompanied by chronic periodontitis.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Wang,Bing Zhou

The Journal of clinical endocrinology and metabolism 109:1517-1525 PubMed38127960

2023

Increased Thyroid DPP4 Expression Is Associated With Inflammatory Process in Patients With Hashimoto Thyroiditis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohui Wen,Xiaona Chang,Xueqing He,Qingyun Cai,Guang Wang,Jia Liu

Frontiers in immunology 14:1063838 PubMed36875091

2023

T-cells engineered with a novel VHH-based chimeric antigen receptor against CD19 exhibit comparable tumoricidal efficacy to their FMC63-based counterparts.

Applications

Unspecified application

Species

Unspecified reactive species

Fatemeh Nasiri,Pooria Safarzadeh Kozani,Fatemeh Rahbarizadeh

Chemotherapy 67:223-233 PubMed35649347

2022

Circular RNA hsa_circ_0068252 Functions in Cisplatin Resistance and Immune Response via miR-1304-5p/PD-L1 Axis in Non-Small Cell Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Li,Jinhua Xu,Guofeng Wu,Yajun Ren,Xue Wang,Qianyun Zhang

Immunity, inflammation and disease 10:218-224 PubMed34847295

2021

LncRNA CCAT1 is overexpressed in tuberculosis patients and predicts their survival.

Applications

Unspecified application

Species

Unspecified reactive species

Taosheng Ye,Jiaohong Zhang,Xuan Zeng,Yuxiang Xu,Jinpei Li
View all publications
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