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AB236895

Human IFN gamma High Sensitivity ELISA Kit

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Human IFN gamma High Sensitivity ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IFN gamma with a sensitivity of 2 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Interferon gamma, IFN-gamma, Immune interferon, IFNG

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ELISA - Human IFN gamma High Sensitivity ELISA Kit (AB236895)
  • ELISA

Supplier Data

ELISA - Human IFN gamma High Sensitivity ELISA Kit (AB236895)
Sandwich ELISA - Human IFN gamma High Sensitivity ELISA Kit (AB236895)
  • sELISA

Supplier Data

Sandwich ELISA - Human IFN gamma High Sensitivity ELISA Kit (AB236895)

Interpolated concentrations of native IFN gamma in human PHA-M stimulated PBMC cell culture supernatant samples.

The concentrations of IFN gamma were measured in duplicates, interpolated from the IFN gamma standard curves and corrected for sample dilution. Undiluted samples are as follows : PBMC supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IFN gamma concentration was determined to be 1102 pg/mL in neat PHA-M stimulated PBMC cell culture supernatant.

Sandwich ELISA - Human IFN gamma High Sensitivity ELISA Kit (AB236895)
  • sELISA

Supplier Data

Sandwich ELISA - Human IFN gamma High Sensitivity ELISA Kit (AB236895)

Comparison of Interferon gamma in unstimulated and PHA-M stimulated PBMC cell culture supernatants.

Human PBMCs were cultured for 46 hours in the presence or absence of 1.5% PHA-M. The concentrations of Interferon gamma were measured in three different dilutions of the supernatant samples in duplicates and interpolated from the Interferon gamma standard curve. The interpolated values are plotted (mean +/- SD, n=3). The mean Interferon gamma concentration was determined to be 1,073 pg/mL in PHA-M stimulated PBMC cell culture supernatant, and undetectable in unstimulated PBMC cell culture supernatant and RPMI1640 media containing 10% FBS (not shown).

Sandwich ELISA - Human IFN gamma High Sensitivity ELISA Kit (AB236895)
  • sELISA

Supplier Data

Sandwich ELISA - Human IFN gamma High Sensitivity ELISA Kit (AB236895)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IFN gamma concentration was determined to be 33.5 pg/mL with a range of non-detectable – 33.5 pg/mL.

Sandwich ELISA - Human IFN gamma High Sensitivity ELISA Kit (AB236895)
  • sELISA

Supplier Data

Sandwich ELISA - Human IFN gamma High Sensitivity ELISA Kit (AB236895)

Human IFN gamma Standard Curve Comparison.

Standard Curve comparison between Human IFN gamma SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human IFN gamma High Sensitivity ELISA Kit (AB236895)
  • sELISA

Supplier Data

Sandwich ELISA - Human IFN gamma High Sensitivity ELISA Kit (AB236895)

Example of human IFN gamma standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IFN gamma High Sensitivity ELISA Kit (AB236895)
  • sELISA

Supplier Data

Sandwich ELISA - Human IFN gamma High Sensitivity ELISA Kit (AB236895)

Example of human IFN gamma standard curve in Sample Diluent 50BP.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Saliva, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 2 pg/mL

Range

12.5 - 1600 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Human IFN gamma High Sensitivity ELISA Kit ab236895 is a rapid single-wash 90-min sandwich ELISA kit to measure Human IFN gamma in Serum, Citrate Plasma, EDTA Plasma, Saliva, and Cell culture supernatant. This Simplestep sensitivity is 2 pg/mL.

How the assay works

Human IFN gamma SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.

This immunoassay is calibrated against a highly purified human IFN gamma. The NIBSC/WHO unclassified purified human IFN gamma preparation 87/586 was evaluated in this kit.
The dose response curve of the unclassified standard IFN gamma parallels the SimpleStep standard curve. To convert sample values obtained with the SimpleStep Human IFN gamma kit to approximate NIBSC 87/586 units, use the equation below.

NIBSC (87/586) approximate value (IU/mL) = 0.01113 x SimpleStep Human IFN gamma value (pg/mL).

Human IFN gamma High Sensitivity ELISA Kit ab236895 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

How other researchers are using Human IFN gamma High Sensitivity ELISA Kit ab236895

Human IFN gamma High Sensitivity ELISA Kit ab236895 has been used to bispecific antibodies as potential cancer therapeutics (1)

References:

(1) X. Pang, et.al., 2022, PMID: 35317514

Related and Recommended products

Human IFN gamma High Sensitivity ELISA Kit ab236895 is commonly used to measure IFN gamma as a marker of inflammation.

Other ELISA kits to measure inflammatory markers include:

- Human TNF alpha ELISA Kit ab181421
- Human IL-6 ELISA Kit ab178013
- Human IL-1 beta ELISA Kit ab214025

To measure IFN gamma in our 384-well format:

- Human Interferon gamma ELISA Kit ab300323
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Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.3" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.6" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "98 - 101 %", "average": "= 100" }, { "sample": "Serum", "range": "122 - 133 %", "average": "= 128" }, { "sample": "EDTA Plasma", "range": "109 - 120 %", "average": "= 115" }, { "sample": "Saliva", "range": "87 - 92 %", "average": "= 90" }, { "sample": "Citrate plasma", "range": "120 - 121 %", "average": "= 121" }, { "sample": "Cell culture media", "range": "91 - 97 %", "average": "= 94" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Interferon gamma (IFN-γ) also known as type II interferon is a cytokine that plays an important role in immune response. IFN-γ has a molecular weight of about 17 kDa and is produced by T cells and natural killer (NK) cells. IFN-γ binds to the interferon gamma receptor initiating a signaling cascade that activates various genes involved in immune functions. It is expressed mainly in activated immune cells within lymphoid tissues and inflamed sites during immune responses.
Biological function summary

This cytokine is significant in promoting macrophage activation enhancing the antigen presentation process and boosting the antimicrobial activity of phagocytes. IFN-γ is not part of a larger protein complex but works as a homodimer in signal transduction. Its production heightens the Th1 immune response by stimulating the differentiation of naïve T cells into Th1 cells which is essential for effective cellular immunity.

Pathways

IFN-γ is integrally involved in the JAK-STAT signaling pathway alongside another critical cytokine Interleukin-12. This pathway further amplifies the immune response by regulating the expression of genes associated with cellular defense mechanisms. IFN-γ also interacts with the NF-kB pathway influencing inflammation and the activation of further immune responses. These interactions show a network of cooperativity with proteins like STAT1 and NF-kB essential for executing its biological roles.

IFN-γ is linked to autoimmune diseases such as rheumatoid arthritis and multiple sclerosis where its elevated levels can exacerbate inflammatory processes. It connects to other proteins like TNF-alpha in promoting the inflammatory cascade. Moreover lower levels of IFN-γ are associated with a heightened risk of infections like tuberculosis demonstrating its vital role in pathogen defense. Therefore understanding IFN-γ and its interactions can be key in developing therapeutic approaches against these conditions.
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Product protocols

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Target data

Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation (PubMed : 16914093, PubMed : 8666937). Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation (PubMed : 8349687). Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription (PubMed : 16914093). Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits (PubMed : 8666937). In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading (PubMed : 8163024). Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference (PubMed : 11112687). Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL (PubMed : 7729559). Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation (By similarity).
See full target information IFNG
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy oncolytics 24:788-798 PubMed35317514

2022

Anticancer effects of a single intramuscular dose of a minicircle DNA vector expressing anti-CD3/CD20 in a xenograft mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojuan Pang,Guochuang Chen,Ping Huang,Peifa Zhang,Jie Liu,Xiaohu Hou,Cheng-Yi He,Ping Chen,Yi-Wu Xie,Jing Zhao,Zhi-Ying Chen
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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