Human IFN gamma High Sensitivity ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human IFN gamma High Sensitivity in Saliva, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
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Publications
- Molecular therapy oncolytics 24:788-7982022Anticancer effects of a single intramuscular dose of a minicircle DNA vector expressing anti-CD3/CD20 in a xenograft mouse model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaojuan Pang,Guochuang Chen,Ping Huang,Peifa Zhang,Jie Liu,Xiaohu Hou,Cheng-Yi He,Ping Chen,Yi-Wu Xie,Jing Zhao,Zhi-Ying ChenPubMed 35317514
Key facts
- Detection method
Colorimetric
- Sample types
Saliva, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
12.5 - 1600 pg/mL
- Assay time
1h 30m
- Sensitivity
= 2 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation (PubMed:16914093, PubMed:8666937). Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation (PubMed:8349687). Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription (PubMed:16914093). Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits (PubMed:8666937). In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading (PubMed:8163024). Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference (PubMed:11112687). Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL (PubMed:7729559). Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation (By similarity).
Alternative names
Interferon gamma, IFN-gamma, Immune interferon, IFNG
What's included?
Recommended products
Human IFN gamma High Sensitivity ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human IFN gamma High Sensitivity in Saliva, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma samples.
Alternative names
Interferon gamma, IFN-gamma, Immune interferon, IFNG
Key facts
- Detection method
Colorimetric
- Sample types
Saliva, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
12.5 - 1600 pg/mL
- Assay time
1h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 2 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Supernatant | n 5 | mean - | SD - | C.V. 2.6 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Supernatant | n 3 | mean - | SD - | C.V. 5.3 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 100 | Range 98 - 101 % |
Sample type Serum | Average % = 128 | Range 122 - 133 % |
Sample type EDTA Plasma | Average % = 115 | Range 109 - 120 % |
Sample type Saliva | Average % = 90 | Range 87 - 92 % |
Sample type Citrate plasma | Average % = 121 | Range 120 - 121 % |
Sample type Cell culture media | Average % = 94 | Range 91 - 97 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Human IFN gamma High Sensitivity ELISA Kit (ab236895) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IFN gamma protein in cit plasma, edta plasma, saliva, serum, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IFN gamma with 2 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Supplementary info
- Activity summary
Interferon gamma (IFN-γ) also known as type II interferon is a cytokine that plays an important role in immune response. IFN-γ has a molecular weight of about 17 kDa and is produced by T cells and natural killer (NK) cells. IFN-γ binds to the interferon gamma receptor initiating a signaling cascade that activates various genes involved in immune functions. It is expressed mainly in activated immune cells within lymphoid tissues and inflamed sites during immune responses.
- Biological function summary
This cytokine is significant in promoting macrophage activation enhancing the antigen presentation process and boosting the antimicrobial activity of phagocytes. IFN-γ is not part of a larger protein complex but works as a homodimer in signal transduction. Its production heightens the Th1 immune response by stimulating the differentiation of naïve T cells into Th1 cells which is essential for effective cellular immunity.
- Pathways
IFN-γ is integrally involved in the JAK-STAT signaling pathway alongside another critical cytokine Interleukin-12. This pathway further amplifies the immune response by regulating the expression of genes associated with cellular defense mechanisms. IFN-γ also interacts with the NF-kB pathway influencing inflammation and the activation of further immune responses. These interactions show a network of cooperativity with proteins like STAT1 and NF-kB essential for executing its biological roles.
- Associated diseases and disorders
IFN-γ is linked to autoimmune diseases such as rheumatoid arthritis and multiple sclerosis where its elevated levels can exacerbate inflammatory processes. It connects to other proteins like TNF-alpha in promoting the inflammatory cascade. Moreover lower levels of IFN-γ are associated with a heightened risk of infections like tuberculosis demonstrating its vital role in pathogen defense. Therefore understanding IFN-γ and its interactions can be key in developing therapeutic approaches against these conditions.
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6 product images
Sandwich ELISA - Human IFN gamma High Sensitivity ELISA Kit (ab236895)
Human IFN gamma Standard Curve Comparison.
Standard Curve comparison between Human IFN gamma SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
Sandwich ELISA - Human IFN gamma High Sensitivity ELISA Kit (ab236895)
Example of human IFN gamma standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human IFN gamma High Sensitivity ELISA Kit (ab236895)
Example of human IFN gamma standard curve in Sample Diluent 50BP.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human IFN gamma High Sensitivity ELISA Kit (ab236895)
Interpolated concentrations of native IFN gamma in human PHA-M stimulated PBMC cell culture supernatant samples.
The concentrations of IFN gamma were measured in duplicates, interpolated from the IFN gamma standard curves and corrected for sample dilution. Undiluted samples are as follows: PBMC supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IFN gamma concentration was determined to be 1102 pg/mL in neat PHA-M stimulated PBMC cell culture supernatant.
Sandwich ELISA - Human IFN gamma High Sensitivity ELISA Kit (ab236895)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IFN gamma concentration was determined to be 33.5 pg/mL with a range of non-detectable – 33.5 pg/mL.
Sandwich ELISA - Human IFN gamma High Sensitivity ELISA Kit (ab236895)
Comparison of Interferon gamma in unstimulated and PHA-M stimulated PBMC cell culture supernatants.
Human PBMCs were cultured for 46 hours in the presence or absence of 1.5% PHA-M. The concentrations of Interferon gamma were measured in three different dilutions of the supernatant samples in duplicates and interpolated from the Interferon gamma standard curve. The interpolated values are plotted (mean +/- SD, n=3). The mean Interferon gamma concentration was determined to be 1,073 pg/mL in PHA-M stimulated PBMC cell culture supernatant, and undetectable in unstimulated PBMC cell culture supernatant and RPMI1640 media containing 10% FBS (not shown).
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