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AB46048

Human IFN gamma High Sensitivity ELISA Kit

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(5 Publications)

Human IFN gamma High Sensitivity ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human IFN gamma High Sensitivity in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Interferon gamma, IFN-gamma, Immune interferon, IFNG

5 Images
ELISA - Human IFN gamma High Sensitivity ELISA Kit (AB46048)
  • ELISA

Lab

ELISA - Human IFN gamma High Sensitivity ELISA Kit (AB46048)

Dilution curves of IFN gamma (ab46048) and NIBSC standard (87/586). One ng of standard IFN gamma corresponds to 12.3 (+/- 1.3) IU NIBSC 87/586. Background signal subtracted (duplicates; +/- SD).

ELISA - Human IFN gamma High Sensitivity ELISA Kit (AB46048)
  • ELISA

Lab

ELISA - Human IFN gamma High Sensitivity ELISA Kit (AB46048)

IFN gamma measured in cell culture supernatants from control or treated (48 hours; 2% PHA-M; LifeTechnologies) PBMCs, diluted 1/100-1/10000 (duplicates +/- SD).

Sandwich ELISA - Human IFN gamma High Sensitivity ELISA Kit (AB46048)
  • sELISA

Unknown

Sandwich ELISA - Human IFN gamma High Sensitivity ELISA Kit (AB46048)

Representative Standard Curve using ab46048

ELISA - Human IFN gamma High Sensitivity ELISA Kit (AB46048)
  • ELISA

Lab

ELISA - Human IFN gamma High Sensitivity ELISA Kit (AB46048)

IFN gamma measured in undiluted human biological fluids; mouse and rat samples (plasma and serum) were below limit of detection (duplicates +/- SD).

ELISA - Human IFN gamma High Sensitivity ELISA Kit (AB46048)
  • ELISA

Lab

ELISA - Human IFN gamma High Sensitivity ELISA Kit (AB46048)

IFN gamma measured in undiluted cell culture supernatants from control or stimulated (48 hours with 50 ng x mL-1 PMA (ab120297) and 1 μM ionomycin (ab120116)) Jurkat cells. Control sample was below limit of detection (<0.57 pg x mL-1) (duplicates +/- SD).

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 0.69 pg/mL

Range

0.78 - 25 pg/mL

Assay time

3h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's IFN gamma Human High Sensitivity in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IFN gamma in Human sera, plasmas, buffered solutions and cell culture media.

A monoclonal antibody specific for IFN gamma has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IFN gamma concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IFN gamma are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IFN gamma present in the samples.

This kit will recognize both endogenous and recombinant Human IFN gamma.

Get results in 90 minutes with Human IFN gamma ELISA Kit (ab174443) from our SimpleStep ELISA® range.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.6" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.9" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": null, "average": "= 108" }, { "sample": "Serum", "range": null, "average": "= 92" }, { "sample": "Plasma", "range": null, "average": "= 72" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Interferon gamma (IFN-γ) also known as type II interferon is a cytokine that plays an important role in immune response. IFN-γ has a molecular weight of about 17 kDa and is produced by T cells and natural killer (NK) cells. IFN-γ binds to the interferon gamma receptor initiating a signaling cascade that activates various genes involved in immune functions. It is expressed mainly in activated immune cells within lymphoid tissues and inflamed sites during immune responses.
Biological function summary

This cytokine is significant in promoting macrophage activation enhancing the antigen presentation process and boosting the antimicrobial activity of phagocytes. IFN-γ is not part of a larger protein complex but works as a homodimer in signal transduction. Its production heightens the Th1 immune response by stimulating the differentiation of naïve T cells into Th1 cells which is essential for effective cellular immunity.

Pathways

IFN-γ is integrally involved in the JAK-STAT signaling pathway alongside another critical cytokine Interleukin-12. This pathway further amplifies the immune response by regulating the expression of genes associated with cellular defense mechanisms. IFN-γ also interacts with the NF-kB pathway influencing inflammation and the activation of further immune responses. These interactions show a network of cooperativity with proteins like STAT1 and NF-kB essential for executing its biological roles.

IFN-γ is linked to autoimmune diseases such as rheumatoid arthritis and multiple sclerosis where its elevated levels can exacerbate inflammatory processes. It connects to other proteins like TNF-alpha in promoting the inflammatory cascade. Moreover lower levels of IFN-γ are associated with a heightened risk of infections like tuberculosis demonstrating its vital role in pathogen defense. Therefore understanding IFN-γ and its interactions can be key in developing therapeutic approaches against these conditions.
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Product protocols

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Target data

Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation (PubMed : 16914093, PubMed : 8666937). Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation (PubMed : 8349687). Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription (PubMed : 16914093). Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits (PubMed : 8666937). In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading (PubMed : 8163024). Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference (PubMed : 11112687). Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL (PubMed : 7729559). Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation (By similarity).
See full target information IFNG
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 14:1326927 PubMed38250068

2024

SON-1210 - a novel bifunctional IL-12 / IL-15 fusion protein that improves cytokine half-life, targets tumors, and enhances therapeutic efficacy.

Applications

Unspecified application

Species

Unspecified reactive species

John K Cini,Susan Dexter,Darrel J Rezac,Stephen J McAndrew,Gael Hedou,Rich Brody,Rukiye-Nazan Eraslan,Richard T Kenney,Pankaj Mohan

Immunity, inflammation and disease 11:e1059 PubMed37904699

2023

The role of inflammatory factors and T-cell subsets in the diagnosis of recurrence in epithelial ovarian cancer patients and the effect of olaparin treatment on them.

Applications

Unspecified application

Species

Unspecified reactive species

Gulijianati Maowulieti,Shaojie Zhao,Min Zhao,Hua Yuan

Frontiers in medicine 9:896409 PubMed36160124

2022

Alterations in bile acids as metabolic signatures in the patients with human adenovirus type 7 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Xu,Juan Du,Ting-Ting Wei,Lin-Yi Chen,Xin-Xin Yang,Tu Bo,Han-Yu Liu,Ming-Zhu Xie,Tian-Shuo Zhao,Jun-Lian Yang,Fuqiang Cui,Wei-Wei Chen,Qing-Bin Lu

Molecular medicine reports 23: PubMed33300058

2020

Penehyclidine hydrochloride alleviates lipopolysaccharide‑induced acute respiratory distress syndrome in cells via regulating autophagy‑related pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaopeng Wang,Fen Liu,Min Xu,Liangxia Wu

International journal of biological sciences 15:1664-1675 PubMed31360109

2019

CD44 3'-Untranslated Region Functions as a Competing Endogenous RNA to Enhance NK Sensitivity of Liver Cancer Stem Cell by Regulating ULBP2 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Weng,Xu Han,Kaiyu Liu,Jiong Yang,Shiruo Wei,Yue Zhang,Fanhong Zeng,Yang Li,Li Shen,Yi Gao
View all publications
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