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AB137980

Human IgA ELISA Kit

5

(1 Review)

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(5 Publications)

Human IgA ELISA Kit is a sandwich ELISA designed to quantify Human IgA with a sensitivity of 0.35 ng/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Wide dynamic range - quantifies 1.56 - 100 ng/mL

View Alternative Names

Immunoglobulin heavy constant alpha 1, Ig alpha-1 chain C region, Ig alpha-1 chain C region BUR, Ig alpha-1 chain C region TRO, IGHA1

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Sandwich ELISA - Human IgA ELISA Kit (AB137980)
  • sELISA

Supplier Data

Sandwich ELISA - Human IgA ELISA Kit (AB137980)

Representative Standard Curve using ab137980

Sandwich ELISA - Human IgA ELISA Kit (AB137980)
  • sELISA

Unknown

Sandwich ELISA - Human IgA ELISA Kit (AB137980)

IgA measured in biological fluids, background signal subtracted (duplicates +/- SD).

Key facts

Detection method

Colorimetric

Sample types

Saliva, Urine, Plasma, Cell culture supernatant, Milk, Serum, Cerebral Spinal Fluid

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.35 ng/mL

Range

1.56 - 100 ng/mL

Assay time

4h

Assay Platform

Microplate

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Reactivity data

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Product details

Human IgA ELISA Kit ab137980 is a sandwich ELISA to measure Human IgA in serum, plasma, cell culture supernatant, cerebral spinal fluid (CSF), saliva, urine, milk with a sensitivity of 0.35 ng/ml.

How the assay works

An IgA specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an IgA specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IgA captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human IgA ELISA Kit ab137980 protocol summary

1. Add standard or sample to appropriate wells. Incubate at room temperature
2. Wash and add prepared biotin antibody to each well. Incubate at room temperature.
3. Wash and add prepared Streptavidin-Peroxidase Conjugate. Incubate at room temperature
4. Add Chromogen Substrate to each well. Incubate at room temperature
5. Add Stop Solution to each well. Read at 450 nm immediately.

Get results in 90 minutes with our SimpleStep ELISA® kit

Human IgA ELISA Kit (ab196263)
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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.1" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.7" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Immunoglobulin A (IgA) also known as sIgA in its secretory form is an important component of the immune system. With a molecular weight of approximately 160 kDa IgA exists as a monomeric or dimeric structure. It is primarily expressed in mucosal areas such as the respiratory tract gastrointestinal tract and urogenital tract while also being present in secretions like saliva tears and breast milk. In its dimeric form IgA associates with a joining (J) chain and a secretory component which facilitates its transport across mucosal barriers.
Biological function summary

IgA functions as a first line of defense in immune responses occurring at mucosal surfaces. Its primary role is to neutralize pathogens toxins and prevent their attachment and penetration through epithelial cells. This antibody does not form part of a larger complex but plays an important role in immune exclusion by trapping antigens in the mucus layer. Additionally IgA can mediate antibody-dependent cellular cytotoxicity and engage with specific receptors like FcαRI (CD89) on immune cells further enhancing its protective capabilities.

Pathways

IgA participates in the mucosal immune response which is a sub-component of the broader immune system pathways. It is closely associated with the polymeric immunoglobulin receptor (pIgR) which transports IgA to the mucosal surfaces. The pathway involves recombination activation proteins (RAG) during its class-switching process in B cells differentiating from IgM-producing cells to those secreting IgA. These pathways are integral to maintaining the mucosal environment and protecting against pathogens.

Alterations or deficiencies in IgA can lead to increased susceptibility to infections and disorders like IgA nephropathy and celiac disease. In IgA nephropathy IgA deposits in the kidneys result in inflammation and damage to renal tissues. Celiac disease involves an immune reaction to gluten where IgA antibodies against tissue transglutaminase (tTG) contribute to intestinal damage. Both conditions illustrate the importance of IgA in maintaining immune balance and its connection with disease pathologies.
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Product protocols

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Target data

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268). Ig alpha is the major immunoglobulin class in body secretions (PubMed : 2241915).
See full target information IGHA1
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Brazilian oral research 38:e043 PubMed38747830

2024

Salivary immunoglobulin levels and periodontal indices in Brazilian children with and without type 1 diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Thyciana Rodrigues Ribeiro,Sara Maria Silva,Renata Asfor Rocha Carvalho Martins,Cláudia Ferreira Santos,Paulo Goberlânio de Barros Silva,Adriana Costa E Forti,Fábio Wildson Gurgel Costa,Manassés Claudino Fonteles,Cristiane Sá Roriz Fonteles

BMC nephrology 24:160 PubMed37286948

2023

Serum levels of galactose-deficient IgA are elevated in patients with IgA nephropathy but do not correlate to disease activity or progression.

Applications

Unspecified application

Species

Unspecified reactive species

Sigridur Elíasdóttir,Alina Khramova,Aso Saeed,Gregor Guron,Roberto Boi,Johan Mölne,Kerstin Ebefors,Jenny Nyström

Archives of Razi Institute 77:1587-1591 PubMed37123123

2022

Tracing Some Salivary Immune Elements in Iraqi SARS-2 Patients.

Applications

Unspecified application

Species

Unspecified reactive species

S Qaysar Musa,M Mohammed AliJassim,M Mohammed Mahmood

Cell 176:610-624.e18 PubMed30612739

2019

Recirculating Intestinal IgA-Producing Cells Regulate Neuroinflammation via IL-10.

Applications

Unspecified application

Species

Unspecified reactive species

Olga L Rojas,Anne-Katrin Pröbstel,Elisa A Porfilio,Angela A Wang,Marc Charabati,Tian Sun,Dennis S W Lee,Georgina Galicia,Valeria Ramaglia,Lesley A Ward,Leslie Y T Leung,Ghazal Najafi,Khashayar Khaleghi,Beatriz Garcillán,Angela Li,Rickvinder Besla,Ikbel Naouar,Eric Y Cao,Pailin Chiaranunt,Kyle Burrows,Hannah G Robinson,Jessica R Allanach,Jennifer Yam,Helen Luck,Daniel J Campbell,David Allman,David G Brooks,Michio Tomura,Ryan Baumann,Scott S Zamvil,Amit Bar-Or,Marc S Horwitz,Daniel A Winer,Arthur Mortha,Fabienne Mackay,Alexandre Prat,Lisa C Osborne,Clinton Robbins,Sergio E Baranzini,Jennifer L Gommerman

Oncotarget 8:109027-109042 PubMed29312588

2017

Tripterygium Wilfordii inhibits tonsillar IgA production by downregulating IgA class switching in IgA nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Huining Li,Dan Kong,Yangyang Xu,Xiaomei Li,Guodong Yao,Kexin Chen,Qi You,Qingtao Shi,Lei Zhang,Xin Wang,Dawei Yuan,Shusheng Miao,Jingshu Geng,Xiaoming Jin,Hongxue Meng
View all publications
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