Human IgA ELISA Kit is a sandwich ELISA designed to quantify Human IgA with a sensitivity of 0.35 ng/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Wide dynamic range - quantifies 1.56 - 100 ng/mL
Images
Publications
Filter by
- BMC nephrology 24:1602023Serum levels of galactose-deficient IgA are elevated in patients with IgA nephropathy but do not correlate to disease activity or progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSigridur Elíasdóttir,Alina Khramova,Aso Saeed,Gregor Guron,Roberto Boi,Johan Mölne,Kerstin Ebefors,Jenny NyströmPubMed 37286948
- Archives of Razi Institute 77:1587-15912022Tracing Some Salivary Immune Elements in Iraqi SARS-2 Patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesS Qaysar Musa,M Mohammed AliJassim,M Mohammed MahmoodPubMed 37123123
- Cell 176:610-624.e182019Recirculating Intestinal IgA-Producing Cells Regulate Neuroinflammation via IL-10.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOlga L Rojas et. al.PubMed 30612739
- Oncotarget 8:109027-1090422017Tripterygium Wilfordii inhibits tonsillar IgA production by downregulating IgA class switching in IgA nephropathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuining Li et. al.PubMed 29312588
Key facts
- Detection method
- Colorimetric
- Sample types
- Saliva, Urine, Plasma, Cell culture supernatant, Milk, Serum, Cerebral Spinal Fluid
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 1.56 - 100 ng/mL
- Assay time
- 4h
- Sensitivity
- = 0.35 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268). Ig alpha is the major immunoglobulin class in body secretions (PubMed:2241915).
Alternative names
Immunoglobulin heavy constant alpha 1, Ig alpha-1 chain C region, Ig alpha-1 chain C region BUR, Ig alpha-1 chain C region TRO, IGHA1
What's included?
Recommended products
Human IgA ELISA Kit is a sandwich ELISA designed to quantify Human IgA with a sensitivity of 0.35 ng/mL.
- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Wide dynamic range - quantifies 1.56 - 100 ng/mL
Key facts
- Detection method
- Colorimetric
- Sample types
- Saliva, Urine, Plasma, Cell culture supernatant, Milk, Serum, Cerebral Spinal Fluid
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 1.56 - 100 ng/mL
- Assay time
- 4h
- Assay Platform
- Microplate
- Sensitivity
- = 0.35 ng/mL
- General recovery
- 97%
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 0 | C.V. 4.7 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 0 | C.V. 10.1 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
Human IgA in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IgA in plasma, serum, urine, saliva, milk, cerebrospinal fluid and cell culture supernatants.
An IgA specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an IgA specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IgA captured in plate.
Get higher sensitivity in only 90 minutes with Human IgA ELISA Kit (Human IgA ELISA Kit ab196263) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Immunoglobulin A (IgA) also known as sIgA in its secretory form is an important component of the immune system. With a molecular weight of approximately 160 kDa IgA exists as a monomeric or dimeric structure. It is primarily expressed in mucosal areas such as the respiratory tract gastrointestinal tract and urogenital tract while also being present in secretions like saliva tears and breast milk. In its dimeric form IgA associates with a joining (J) chain and a secretory component which facilitates its transport across mucosal barriers.
- Biological function summary
IgA functions as a first line of defense in immune responses occurring at mucosal surfaces. Its primary role is to neutralize pathogens toxins and prevent their attachment and penetration through epithelial cells. This antibody does not form part of a larger complex but plays an important role in immune exclusion by trapping antigens in the mucus layer. Additionally IgA can mediate antibody-dependent cellular cytotoxicity and engage with specific receptors like FcαRI (CD89) on immune cells further enhancing its protective capabilities.
- Pathways
IgA participates in the mucosal immune response which is a sub-component of the broader immune system pathways. It is closely associated with the polymeric immunoglobulin receptor (pIgR) which transports IgA to the mucosal surfaces. The pathway involves recombination activation proteins (RAG) during its class-switching process in B cells differentiating from IgM-producing cells to those secreting IgA. These pathways are integral to maintaining the mucosal environment and protecting against pathogens.
- Associated diseases and disorders
Alterations or deficiencies in IgA can lead to increased susceptibility to infections and disorders like IgA nephropathy and celiac disease. In IgA nephropathy IgA deposits in the kidneys result in inflammation and damage to renal tissues. Celiac disease involves an immune reaction to gluten where IgA antibodies against tissue transglutaminase (tTG) contribute to intestinal damage. Both conditions illustrate the importance of IgA in maintaining immune balance and its connection with disease pathologies.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Sandwich ELISA - Human IgA ELISA Kit (ab137980)
IgA measured in biological fluids, background signal subtracted (duplicates +/- SD).
Sandwich ELISA - Human IgA ELISA Kit (ab137980)
Representative Standard Curve using ab137980
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com