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AB325622

Human IgA ELISA Kit, Chemiluminescent

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Human IgA ELISA Kit, Chemiluminescent is a 55 min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human IgA with higher sensitivity and broader measuring range.

- Single 30 min incubation and single -wash step for results in less than an hour
- Chemiluminescent detection allows a broad dynamic range : 0.14 - 100 ng/ml with a sensitivity of 0.09 ng/mL
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Patent pending

View Alternative Names

Immunoglobulin heavy constant alpha 1, Ig alpha-1 chain C region, Ig alpha-1 chain C region BUR, Ig alpha-1 chain C region TRO, IGHA1

5 Images
Sandwich ELISA - Human IgA ELISA Kit, Chemiluminescent (AB325622)
  • sELISA

Lab

Sandwich ELISA - Human IgA ELISA Kit, Chemiluminescent (AB325622)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human IgA ELISA Kit, Chemiluminescent (AB325622)
  • sELISA

Lab

Sandwich ELISA - Human IgA ELISA Kit, Chemiluminescent (AB325622)

Standard curve comparison between the Chemiluminescent Human IgA SimpleStep ELISA and colorimetric Human IgA SimpleStep ELISA (ab196263) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IgA ELISA Kit, Chemiluminescent (AB325622)
  • sELISA

Lab

Sandwich ELISA - Human IgA ELISA Kit, Chemiluminescent (AB325622)

Dynamic range comparison between the Chemiluminescent Human IgA SimpleStep ELISA, colorimetric Human IgA SimpleStep ELISA (ab196263), and leading chemiluminescent competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human IgA ELISA Kit, Chemiluminescent (AB325622)
  • sELISA

Lab

Sandwich ELISA - Human IgA ELISA Kit, Chemiluminescent (AB325622)

Fluids from a pooled selection of healthy donors were evaluated for the presence of Human IgA in serum, milk, saliva, and urine using this assay. Results were interpolated from the standard curve in Sample Diluent NS and corrected for sample dilution. The mean levels of Human IgA in serum were 2.45 mg/mL, in milk at 0.74 mg/mL, in saliva at 0.94 mg/mL and in urine at 0.05 mg/mL.

Sandwich ELISA - Human IgA ELISA Kit, Chemiluminescent (AB325622)
  • sELISA

Lab

Sandwich ELISA - Human IgA ELISA Kit, Chemiluminescent (AB325622)

Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean IgA concentration was determined to be 3.978 mg/mL with a range of 1.016 – 9.476 mg/mL.

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Urine, Saliva, Milk, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.09 ng/mL

Range

0.14 - 100 ng/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Human IgA ELISA Kit, Chemiluminescent (ab325622) is a rapid single-wash sandwich 55 min ELISA to measure Human IgA in Serum, Citrate Plasma, EDTA Plasma, Heparin Plasma, Urine, Saliva, Milk, Cell Culture Supernatant with a sensitivity of 0.09 ng/mL.

How the assay works

Human IgA ELISA Kit, Chemiluminescent (ab325622) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colormetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human IgA (ab325622) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-IgA antibody [EPR5367-76] - BSA and Azide free (Capture/Detector) ab244893
- Anti-IgA antibody [EPR5367-76] - BSA and Azide free (Capture/Detector) ab244893
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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.2" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.9" } ]
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Recovery

[ { "sample": "Serum", "range": "81 - 109 %", "average": "= 98" }, { "sample": "Citrate plasma", "range": "89 - 99 %", "average": "= 93" }, { "sample": "EDTA Plasma", "range": "75 - 88 %", "average": "= 81" }, { "sample": "Heparin Plasma", "range": "91 - 109 %", "average": "= 97" }, { "sample": "Cell culture media", "range": "85 - 117 %", "average": "= 101" }, { "sample": "Urine", "range": "86 - 109 %", "average": "= 99" }, { "sample": "Saliva", "range": "88 - 92 %", "average": "= 90" }, { "sample": "Milk", "range": "81 - 102 %", "average": "= 92" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268). Ig alpha is the major immunoglobulin class in body secretions (PubMed : 2241915).
See full target information IGHA1
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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