AB326405

Human IgA1 ELISA Kit, Chemiluminescent

SimpleStep Ignite™ ELISA
Recombinant
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Human IgA1 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human IgA1 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 0.04 - 90 ng/ml with a sensitivity of 0.034ng/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
-Patent pending

View Alternative Names

Immunoglobulin heavy constant alpha 1, Ig alpha-1 chain C region, Ig alpha-1 chain C region BUR, Ig alpha-1 chain C region TRO, IGHA1

6 Images

Sandwich ELISA - Human IgA1 ELISA Kit, Chemiluminescent (AB326405)

sELISA

Lab

Sandwich ELISA - Human IgA1 ELISA Kit, Chemiluminescent (AB326405)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

sELISA

Supplier Data

Sandwich ELISA - Human IgA1 ELISA Kit, Chemiluminescent (AB326405)

Interpolated concentration of native IgA1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : urine 1 : 100 milk 1 : 20000 and saliva 1 : 2000. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human IgA1 ELISA Kit, Chemiluminescent (AB326405)

Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean IgA1 concentration was determined to be 1218 µg/mL with a range of 639 – 2204 µg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human IgA1 ELISA Kit, Chemiluminescent (AB326405)

Interpolated concentration of native IgA1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 1 : 62500 plasma (citrate) 1 : 50000 plasma (EDTA) 1 : 50000 and plasma (heparin) 1 : 100000. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Lab

Sandwich ELISA - Human IgA1 ELISA Kit, Chemiluminescent (AB326405)

Standard curve comparison between the Chemiluminescent Human IgA1 SimpleStep ELISA and colorimetric Human IgA1 SimpleStep ELISA (ab316261) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human IgA1 ELISA Kit, Chemiluminescent (AB326405)

Dynamic range comparison between the Chemiluminescent Human IgA1 SimpleStep ELISA, colorimetric Human IgA1 SimpleStep ELISA (ab316261), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased dynamic range.

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Urine, Milk, Saliva, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.034 ng/mL

Range

0.04 - 90 ng/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human IgA1 ELISA Kit, Chemiluminescent (ab326405) is a rapid single-wash sandwich 55-min ELISA to measure Human IgA1 in Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Urine, Milk, Saliva, Cell culture supernatant with a sensitivity of 0.034 ng/mL.

How the assay works

Human IgA1 ELISA Kit, Chemiluminescent (ab326405) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human IgA1 (ab326405) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-IgA1 antibody [EPR26775-50] - BSA and Aside free (Capture) ab312873
- Anti-IgA1 antibody [EPR26775-52] - BSA and Aside free (Detector) ab312874

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.4" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.6" } ]

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Recovery

[ { "sample": "Serum", "range": "111 - 125 %", "average": "= 118" }, { "sample": "Citrate plasma", "range": "94 - 104 %", "average": "= 98" }, { "sample": "EDTA Plasma", "range": "94 - 112 %", "average": "= 104" }, { "sample": "Heparin Plasma", "range": "82 - 99 %", "average": "= 90" }, { "sample": "Urine", "range": "87 - 97 %", "average": "= 93" }, { "sample": "Milk", "range": "96 - 100 %", "average": "= 99" }, { "sample": "Saliva", "range": "88 - 95 %", "average": "= 91" }, { "sample": "Cell culture supernatant", "range": "82 - 84 %", "average": "= 83" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268). Ig alpha is the major immunoglobulin class in body secretions (PubMed : 2241915).

See full target information IGHA1

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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