Human IgD ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IgD with a sensitivity of 10.34 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media, Urine, Saliva, Milk
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 42.188 - 2700 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 10.34 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268). IgD is the major antigen receptor isotype on the surface of most peripheral B-cells, where it is coexpressed with IgM. The membrane-bound IgD (mIgD) induces the phosphorylation of CD79A and CD79B by the Src family of protein tyrosine kinases. Soluble IgD (sIgD) concentration in serum below those of IgG, IgA, and IgM but much higher than that of IgE. IgM and IgD molecules present on B cells have identical V regions and antigen-binding sites. After the antigen binds to the B-cell receptor, the secreted form sIgD is shut off. IgD is a potent inducer of TNF, IL1B, and IL1RN. IgD also induces release of IL6, IL10, and LIF from peripheral blood mononuclear cells. Monocytes seem to be the main producers of cytokines in vitro in the presence of IgD (PubMed:10702483, PubMed:11282392, PubMed:8774350).
Alternative names
Immunoglobulin heavy constant delta, Ig delta chain C region, Ig delta chain C region NIG-65, Ig delta chain C region WAH, IGHD
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Human IgD ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IgD with a sensitivity of 10.34 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media, Urine, Saliva, Milk
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 42.188 - 2700 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 10.34 pg/mL
- General recovery
- 107%
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. 3.1 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 3.5 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 107 | Range 105 - 109 % |
Sample type Citrate plasma | Average % = 105 | Range 101 - 112 % |
Sample type EDTA Plasma | Average % = 104 | Range 93 - 114 % |
Sample type Heparin Plasma | Average % = 110 | Range 101 - 116 % |
Sample type Cell culture media | Average % = 95 | Range 90 - 102 % |
Sample type Urine | Average % = 99 | Range 97 - 102 % |
Sample type Saliva | Average % = 87 | Range 86 - 89 % |
Sample type Milk | Average % = 108 | Range 106 - 110 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human IgD SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IgD protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture media, Urine, Saliva, Milk. Quantitate Human IgD with 10.34 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IgD also known as Immunoglobulin D serves as a distinct antibody isotype expressed primarily on the surface of B cells. It is involved in the initiation of immune responses. IgD has a molecular weight of approximately 180 kDa. The full form of IgD is Immunoglobulin D. It is expressed mainly in the respiratory system the tonsils and lymphoid tissues playing a pivotal role in the early stages of immunity. Researchers often perform IgD tests to study its mechanical aspects and influence on immune cell functionality.
- Biological function summary
IgD contributes to the activation and differentiation of B lymphocytes and may influence antibody affinity maturation. This immunoglobulin is not known to form part of a larger complex but acts in conjunction with other immunoglobulins like IgM to regulate immune responses. The presence of IgD on B cells suggests its involvement in signaling processes which help B cells transition from immature to fully functional states impacting how the immune system responds to pathogens.
- Pathways
IgD plays roles in immune system signaling and pathogen recognition. It appears in pathways such as B cell receptor signaling and participates in complex interactions with other proteins such as IgM and CD79 which are essential for transmitting activation signals. These pathways are important enabling the immune system to identify and respond to antigens effectively offering a first line of defense against infections.
- Associated diseases and disorders
IgD has associations with recurrent respiratory infections and IgD myeloma a rare form of plasma cell neoplasm. The malfunction or dysregulation of IgD can impact B cell development and lead to immune system disorders. In such cases IgD levels often measured through IgD tests may serve as important indicators for monitoring disease progression. Moreover elevated levels of IgD can coexist with proteins such as IgE particularly in allergy-related responses and play roles in certain immunodeficiency conditions.
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5 product images
Sandwich ELISA - Human IgD ELISA Kit (ab320794)
Example of human IgD standard curve in Sample Diluent NS. Example of human IgD standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human IgD ELISA Kit (ab320794)
Interpolated concentrations of human IgD in serum, plasma (citrate), plasma EDTA, and plasma heparin. Interpolated concentration of native IgD was measured in duplicate at different sample concentrations. Undiluted samples are as follows: 1:10,000 serum, 1:10,000 plasma citrate, 1:5,000 plasma EDTA, and 1:10,000 plasma heparin. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human IgD ELISA Kit (ab320794)
Interpolated concentrations of human IgD in milk. Interpolated concentration of native IgD was measured in duplicate at different sample concentrations. Undiluted sample is 1:100 milk. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human IgD ELISA Kit (ab320794)
Example of human IgD standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Example of human IgD standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human IgD ELISA Kit (ab320794)
Interpolated concentrations of human IgD in serum in 96-well vs. 384-well plates. Interpolated concentration of native IgD was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1:20,000 serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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