AB317776

Human IgE ELISA Kit

SimpleStep
Recombinant
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Human IgE ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IgE with a sensitivity of 23.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Immunoglobulin heavy constant epsilon, Ig epsilon chain C region, Ig epsilon chain C region ND, IGHE

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Sandwich ELISA - Human IgE ELISA Kit (AB317776)

sELISA

Supplier Data

Sandwich ELISA - Human IgE ELISA Kit (AB317776)

Interpolated concentrations of human IgE in serum, plasma (citrate), and plasma (EDTA). Interpolated concentration of native IgE was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 40 human serum, plasma (citrate), and plasma (EDTA). The interpolated dilution factor corrected values (to neat samples) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.

sELISA

Supplier Data

Sandwich ELISA - Human IgE ELISA Kit (AB317776)

Interpolated concentrations of IgE in normal human serum donors. Serum of nine individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IgE concentration was determined to be 115.5 ng/mL with a range of 25.3 - 303.6 ng/mL.

sELISA

Supplier Data

Sandwich ELISA - Human IgE ELISA Kit (AB317776)

Example of human IgE standard curve in Sample Diluent NS + 1X Enhancer. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human IgE ELISA Kit (AB317776)

Interpolated concentrations of human IgE in serum in 96-well vs. 384-well plates. Interpolated concentration of native IgE was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 40 serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.

sELISA

Supplier Data

Sandwich ELISA - Human IgE ELISA Kit (AB317776)

Interpolated concentrations of IgE in atopic dermatitis human serum donors. Serum of three individual human donors with atopic dermatitis as measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IgE concentration was determined to be 260.2 ng/mL with a range of 13.9 - 687.2 ng/mL.

sELISA

Supplier Data

Sandwich ELISA - Human IgE ELISA Kit (AB317776)

Example of human IgE standard curve in Sample Diluent NS + 1X Enhancer in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human IgE ELISA Kit (AB317776)

Representative image of Human IgE ELISA Kit (ab317776)

Components shown from left to right :

- Sample diluent NS

- Denaturant

- 10X Human IgE Capture Antibody

- 10X Human IgE Detector Antibody

- Human IgE Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Stop Solution

- TMB solution

- 10X Wash Buffer PT (ab206977)

- Antibody Diluent 5BR

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma, Cell culture media, Urine, Saliva, Citrate plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 23.1 pg/mL

Range

109.375 - 7000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Product details

Human IgE SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IgE protein in Serum, Cit Plasma, EDTA Plasma, Cell culture media, Urine, Saliva. Quantitate Human IgE with 23.1 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.4" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.6" } ]

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Recovery

[ { "sample": "Serum", "range": "77 - 84 %", "average": "= 81" }, { "sample": "Citrate plasma", "range": "89 - 101 %", "average": "= 97" }, { "sample": "EDTA Plasma", "range": "80 - 88 %", "average": "= 85" }, { "sample": "Cell culture media", "range": "90 - 94 %", "average": "= 93" }, { "sample": "Urine", "range": "98 - 103 %", "average": "= 100" }, { "sample": "Saliva", "range": "93 - 99 %", "average": "= 97" } ]

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What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab317776-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB317776-CMP04" }, { "size":"10 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB317776-CMP12" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB317776-CMP02" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB317776-CMP03" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB317776-CMP10" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB317776-CMP13" }, { "size":"20 x 1 Vial", "name":"Human IgE Lyophilized Recombinant Protein", "number":"AB317776-CMP09" }, { "size":"10 x 600 µL", "name":"10X Human IgE Capture Antibody", "number":"AB317776-CMP01" }, { "size":"10 x 600 µL", "name":"10X Human IgE Detector Antibody", "number":"AB317776-CMP08" }, { "size":"10 x 500 µL", "name":"Denaturant", "number":"AB317776-CMP11" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB317776-CMP05" }, { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB317776-CMP06" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab317776-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB317776-CMP04" }, { "size":"1 x 5 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB317776-CMP12" }, { "size":"2 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB317776-CMP02" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB317776-CMP03" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB317776-CMP10" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB317776-CMP13" }, { "size":"2 x 1 Vial", "name":"Human IgE Lyophilized Recombinant Protein", "number":"AB317776-CMP09" }, { "size":"1 x 600 µL", "name":"10X Human IgE Capture Antibody", "number":"AB317776-CMP01" }, { "size":"1 x 600 µL", "name":"10X Human IgE Detector Antibody", "number":"AB317776-CMP08" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB317776-CMP07" }, { "size":"1 x 500 µL", "name":"Denaturant", "number":"AB317776-CMP11" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB317776-CMP06" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab317776-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB317776-CMP04", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB317776-CMP12", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB317776-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB317776-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB317776-CMP10", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB317776-CMP13", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IgE Lyophilized Recombinant Protein", "number":"AB317776-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IgE Capture Antibody", "number":"AB317776-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IgE Detector Antibody", "number":"AB317776-CMP08", "productcode":"" }, { "size":"1 x 500 µL", "name":"Denaturant", "number":"AB317776-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB317776-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB317776-CMP06", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The IgE protein [also known as immunoglobulin E] is a type of antibody with a molecular mass of approximately 190 kDa. IgE is expressed primarily on the surface of mast cells and basophils. It plays a central role in the immune system by binding to allergens and triggering the release of inflammatory mediators from mast cells and basophils. IgE's high-affinity binding to its receptor FcεRI on these effector cells facilitates this process. IgE's activity is central to initiating immediate hypersensitivity reactions which are a part of the body's immune response.

Biological function summary

The IgE protein functions as a mediator in allergic reactions. It does not operate as part of a complex but directly interacts with allergens and the IgE-specific receptors on immune cells. IgE's role is important in defense against parasitic infections where it performs a protective function by promoting eosinophil activation and degranulation. This action helps the body to expel the parasites. In the absence of parasitic infection IgE's function translates into its role in allergic responses where it becomes responsible for the symptoms associated with allergic diseases.

Pathways

IgE is an important player in the immune response pathway. The protein interacts with allergy-related pathways such as the T helper 2 (Th2) pathway. IgE's role in this pathway involves cooperation with cytokines notably IL-4 and IL-13 which are produced by Th2 cells and promote IgE synthesis in B cells. Also IgE is linked to the FcεRI receptor signaling pathway that initiates the release of histamines and other mediators when IgE binds to an allergen leading to an allergic response.

IgE is closely linked to allergic conditions like asthma and allergic rhinitis. These conditions result from IgE-mediated inflammatory responses that affect the respiratory system. In asthma IgE antibodies play a role in airway inflammation and hyperreactivity by promoting the activation of multiple immune cells including eosinophils and mast cells. IgE's involvement in allergic rhinitis manifests through similar mechanisms with nasal inflammation and irritation. IgE's interaction with other proteins such as histamine-releasing factor exacerbates these conditions by increasing the release of histamine which intensifies the allergic symptoms.

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Product protocols

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Target data

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268).. Isoform 1. Constant region of secreted IgE, also known as the Fc region of IgE antibody. Mediates IgE effector functions on myeloid and lymphoid cells primarily via two Fc receptors, the high-affinity IgE Fc receptor complex/FCER1A : MS4A2 : FCGR1A and the low-affinity FCER2 receptor, which upon antigen/allergen cross-linking initiate signaling pathways that lead to immune cell activation and differentiation (PubMed : 2167225, PubMed : 25629393, PubMed : 33840121, PubMed : 7544003, PubMed : 8114916, PubMed : 8551243). Triggers the immediate hypersensitivity response to allergens as a host defense mechanism against helminth parasites, pathogenic bacteria and venom toxicity. When dysregulated, it can elicit harmful life-threatening allergic and anaphylactic reactions (PubMed : 25629393, PubMed : 33840121, PubMed : 7544003, PubMed : 8114916, PubMed : 8551243). Stimulates the high-affinity IgE Fc receptor complex/FCER1A : MS4A2 : FCGR1A on mast cells, basophils and eosinophils leading to secretion of vasoactive amines, lipid mediators and cytokines that contribute to inflammatory response, tissue remodeling and cytotoxicity against microbes (PubMed : 25629393, PubMed : 8114916, PubMed : 8551243). On macrophages, cross-linking of FCER2 by IgE immune complexes induces intracellular killing of parasites through activation of L-Arginine-nitric oxide pathway (PubMed : 7544003). Activates macrophages to kill tumor cells via antigen-specific antibody-dependent cytotoxicity (ADCC). Triggers differentiation of quiescent M0 macrophages toward M1 state and reprograms M2 macrophages toward a proinflammatory state with antitumor functions (PubMed : 30956175). Stimulates FCER2 on B cells and initiates IgE-dependent antigen uptake and presentation to T cells (PubMed : 2167225).. Isoform 2. Constant region of membrane-bound IgE (long mIgE), part of the B cell receptor complex (BCR). Upon antigen cross-linking triggers quick BCR signaling, ensuring survival of IgE-switched B cells and differentiation into plasma cells, thus regulating both primary and memory IgE responses.. Isoform 3. Constant region of membrane-bound IgE (short mIgE), part of the B cell receptor complex (BCR). Upon antigen cross-linking initiates slower but sustained BCR signaling that negatively regulates mature B cell proliferation.

See full target information IGHE

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human IgE ELISA Kit

Sandwich ELISA - Human IgE ELISA Kit (AB317776)

Sandwich ELISA - Human IgE ELISA Kit (AB317776)

Sandwich ELISA - Human IgE ELISA Kit (AB317776)

Sandwich ELISA - Human IgE ELISA Kit (AB317776)

Sandwich ELISA - Human IgE ELISA Kit (AB317776)

Sandwich ELISA - Human IgE ELISA Kit (AB317776)

Sandwich ELISA - Human IgE ELISA Kit (AB317776)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Secondary Antibodies

Primary Antibodies

Assay Kits

Assay Kits

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise