AB211651

Human IGF1 ELISA Kit

SimpleStep
What is this?

Be the first to review this product! Submit a review

(12 Publications)

Human IGF1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IGF1 with a sensitivity of 11.97 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 10 citations

View Alternative Names

IBP1, IGF-1, IGF1, Insulin-like growth factor 1, Insulin-like growth factor I, Mechano growth factor, Somatomedin-C, IGF-I, MGF

13 Images

ELISA

Supplier Data

ELISA - Human IGF1 ELISA Kit (AB211651)

sELISA

Supplier Data

Sandwich ELISA - Human IGF1 ELISA Kit (AB211651)

Representative image of Human IGF1 ELISA Kit (ab211651)

Components shown from left to right :

- Sample diluent NS

- Denaturant

- 10X Human IGF1 Capture Antibody

- 10X Human IGF1 Detector Antibody

- Human IGF1 Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Stop Solution

- TMB solution

- 10X Wash Buffer PT (ab206977)

- Antibody Diluent 4BR

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

sELISA

Supplier Data

Sandwich ELISA - Human IGF1 ELISA Kit (AB211651)

Example of human IGF1 standard curve in Sample Diluent NS + 1X Enhancer. The IGF1 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human IGF1 ELISA Kit (AB211651)

Example of human IGF1 standard curve in Sample Diluent NS + 1X Enhancer.

The IGF1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human IGF1 ELISA Kit (AB211651)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Native IGF1 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS + 1X Enhancer.

sELISA

Supplier Data

Sandwich ELISA - Human IGF1 ELISA Kit (AB211651)

1% Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGF1 concentration was determined to be 78.06 pg/mL with a range of 45.41 – 141.07 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human IGF1 ELISA Kit (AB211651)

1% Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGF1 concentration was determined to be 129.12 ng/mL with a range of 69.09-184.22 ng/mL.

sELISA

Lab

Sandwich ELISA - Human IGF1 ELISA Kit (AB211651)

Interpolated concentration of native IGF1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 2.5% serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.

sELISA

Supplier Data

Sandwich ELISA - Human IGF1 ELISA Kit (AB211651)

Interpolated concentrations of native IGF1 in human serum and plasma samples.

The concentrations of IGF1 were measured in duplicates, interpolated from the IGF1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 2.5%, plasma (citrate) 1.25%, plasma (EDTA) 1.25%, and plasma (heparin) 1.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGF1 concentration was determined to be 99.58 ng/mL in serum, 121.23 pg/mL in plasma (citrate), 103.65 ng/mL in plasma (EDTA), and 110.43 ng/mL in plasma (heparin).

sELISA

Lab

Sandwich ELISA - Human IGF1 ELISA Kit (AB211651)

Example of human IGF1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human IGF1 ELISA Kit (AB211651)

Human IGF1 competitor curve comparison.

Standard Curve comparison between human IGF1 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Human IGF1 ELISA Kit (AB211651)

Human IGF1 standard curve comparison.

Standard Curve comparison between the original human IGF1 SimpleStep ELISA kit and current human IGF1 SimpleStep ELISA kit.

sELISA

Supplier Data

Sandwich ELISA - Human IGF1 ELISA Kit (AB211651)

Linearity of dilution.

Recombinant IGF1 was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS + 1X Enhancer.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 11.97 pg/mL

Range

93.75 - 6000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Human IGF1 ELISA Kit ab211651 is a rapid single-wash 90-min Sandwich ELISA to measure Human IGF1 in cell culture media, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 11.97 pg/mL.

How the assay works

Human IGF1 SimpleStep ELISA^®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human IGF1 ELISA Kit ab211651 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our 384-well Human IGF1 ELISA Kit only requires a maximum of 12.5µL sample

Insulin-like growth factor I (IGF1) is a 70 amino acid hormone that this proteolytically processed from a larger precursor protein. IGF1 is structurally similar to insulin and has growth promoting effects.

style

left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.5" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.9" } ]

style

left-column

Recovery

[ { "sample": "Serum", "range": "83 - 90 %", "average": "= 86" }, { "sample": "EDTA Plasma", "range": "106 - 118 %", "average": "= 113" }, { "sample": "Cell culture media", "range": "96 - 104 %", "average": "= 100" }, { "sample": "Heparin Plasma", "range": "91 - 111 %", "average": "= 100" }, { "sample": "Citrate plasma", "range": "97 - 104 %", "average": "= 100" } ]

style

left-column

What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab211651-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB211651-CMP05" }, { "size":"10 x 500 µL", "name":"Denaturant", "number":"AB211651-CMP06" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB211651-CMP04" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB211651-CMP11" }, { "size":"10 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB211651-CMP07" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB211651-CMP02" }, { "size":"10 x 600 µL", "name":"10X Human IGF1 Detector Antibody", "number":"AB211651-CMP09" }, { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB211651-CMP10" }, { "size":"10 x 600 µL", "name":"10X Human IGF1 Capture Antibody", "number":"AB211651-CMP08" }, { "size":"20 x 1 Vial", "name":"Human IGF1 Lyophilized Recombinant Protein", "number":"AB211651-CMP12" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB211651-CMP01" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB211651-CMP03" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab211651-1x384Tests", "assetComponentDetails": [ { "size":"1 x 500 µL", "name":"Denaturant", "number":"AB211651-CMP06" }, { "size":"2 x 1 Vial", "name":"Human IGF1 Lyophilized Recombinant Protein", "number":"AB211651-CMP12" }, { "size":"1 x 600 µL", "name":"10X Human IGF1 Capture Antibody", "number":"AB211651-CMP08" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB211651-CMP11" }, { "size":"1 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB211651-CMP02" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB211651-CMP01" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB211651-CMP10" }, { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB211651-CMP04" }, { "size":"1 x 600 µL", "name":"10X Human IGF1 Detector Antibody", "number":"AB211651-CMP09" }, { "size":"6 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB211651-CMP07" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB211651-CMP05" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB211651-CMP13" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab211651-1x96Tests", "assetComponentDetails": [ { "size":"1 x 500 µL", "name":"Denaturant", "number":"AB211651-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB211651-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB211651-CMP11", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB211651-CMP07", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB211651-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB211651-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB211651-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IGF1 Lyophilized Recombinant Protein", "number":"AB211651-CMP12", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB211651-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IGF1 Capture Antibody", "number":"AB211651-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB211651-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IGF1 Detector Antibody", "number":"AB211651-CMP09", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The insulin-like growth factor 1 or IGF1 is a protein hormone that plays an important mechanical role in growth and development. Alternative names for IGF1 include somatomedin C. This protein has a mass of approximately 7.6 kDa and is mainly produced by the liver. IGF1 is expressed in a range of tissues including muscle bone and cartilage reflecting its importance in various physiological processes. Its production is stimulated by growth hormone and it acts in an autocrine and paracrine manner to exert its effects.

Biological function summary

IGF1 stimulates growth and proliferation of cells most notably impacting the development of the skeletal system and the regulation of apoptosis. It is not part of a larger protein complex but it interacts closely with IGF1 receptor to execute its functions. By binding to this receptor IGF1 activates intracellular signaling cascades that promote anabolic effects which include increased uptake of glucose and amino acids contributing to muscle and tissue growth.

Pathways

IGF1 operates within the PI3K-Akt and MAPK signaling pathways which are significant for cell survival and proliferation. Its interaction with proteins like IGF binding protein 3 (IGFBP3) modulates its availability and activity in the bloodstream. These pathways are critical for mediating the effects of growth hormone highlighting the role of IGF1 in systemic growth and metabolism regulation.

Alterations in IGF1 levels associate with growth abnormalities such as Laron syndrome and acromegaly. Laron syndrome results from the body's inability to use IGF1 properly due to receptor defects while acromegaly occurs from excessive IGF1 production often due to pituitary tumors. Furthermore aberrant IGF1 signaling connects to cancer development where it can drive tumor growth and metastasis. Drugs targeting IGF1 and its pathway components continue to be researched for therapeutic potential in these conditions.

style

left-column

Product protocols

style

left-column

Target data

The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation (PubMed : 21076856, PubMed : 24132240). Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb (By similarity). Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV : ITGB3 and ITGA6 : ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1 (PubMed : 19578119, PubMed : 22351760, PubMed : 23243309, PubMed : 23696648). As part of the MAPK/ERK signaling pathway, acts as a negative regulator of apoptosis in cardiomyocytes via promotion of STUB1/CHIP-mediated ubiquitination and degradation of ICER-type isoforms of CREM (By similarity).

See full target information IGF1

style

left-column

Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Stem cells and development 33:484-495 PubMed38940748

2024

Differentiation, Metabolism, and Cardioprotective Secretory Functions of Human Cardiac Stromal Cells from Ischemic and Endocarditis Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Helen Nguyen,Chuan-Chih Hsu,Annette Meeson,Rachel Oldershaw,Gavin Richardson,Andreas Czosseck,David J Lundy

Journal of experimental & clinical cancer research : CR 42:336 PubMed38087324

2023

Interleukin-30 subverts prostate cancer-endothelium crosstalk by fostering angiogenesis and activating immunoregulatory and oncogenic signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Stefania Livia Ciummo,Carlo Sorrentino,Cristiano Fieni,Emma Di Carlo

Journal of controlled release : official journal of the Controlled Release Society 352:879-892 PubMed36370875

2022

Porous scaffold for mesenchymal cell encapsulation and exosome-based therapy of ischemic diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Andreas Czosseck,Max M Chen,Helen Nguyen,Annette Meeson,Chuan-Chih Hsu,Chien-Chung Chen,Thomashire A George,Shu-Chian Ruan,Yuan-Yuan Cheng,Po-Ju Lin,Patrick C H Hsieh,David J Lundy

Nature methods 19:1449-1460 PubMed36280722

2022

Geometric engineering of organoid culture for enhanced organogenesis in a dish.

Applications

Unspecified application

Species

Unspecified reactive species

Sunghee Estelle Park,Shawn Kang,Jungwook Paek,Andrei Georgescu,Jeehan Chang,Alex Yoon Yi,Benjamin J Wilkins,Tatiana A Karakasheva,Kathryn E Hamilton,Dan Dongeun Huh

Journal of hematology & oncology 15:145 PubMed36224639

2022

CRISPR/Cas9-mediated deletion of Interleukin-30 suppresses IGF1 and CXCL5 and boosts SOCS3 reducing prostate cancer growth and mortality.

Applications

Unspecified application

Species

Unspecified reactive species

Carlo Sorrentino,Luigi D'Antonio,Stefania Livia Ciummo,Cristiano Fieni,Lorena Landuzzi,Francesca Ruzzi,Simone Vespa,Paola Lanuti,Lavinia Vittoria Lotti,Pier Luigi Lollini,Emma Di Carlo

Prostate cancer and prostatic diseases 25:86-92 PubMed35152272

2022

Exercise in advanced prostate cancer elevates myokine levels and suppresses in-vitro cell growth.

Applications

Unspecified application

Species

Unspecified reactive species

Jin-Soo Kim,Dennis R Taaffe,Daniel A Galvão,Nicolas H Hart,Elin Gray,Charles J Ryan,Stacey A Kenfield,Fred Saad,Robert U Newton

ACS synthetic biology 10:3359-3368 PubMed34842418

2021

Promoters with Consistent Expression throughout the Murine Gut.

Applications

Unspecified application

Species

Unspecified reactive species

Jeremy Armetta,Michael Schantz-Klausen,Denis Shepelin,Ruben Vazquez-Uribe,Martin Iain Bahl,Martin Frederik Laursen,Tine Rask Licht,Morten O A Sommer

Frontiers in medicine 8:655863 PubMed34295909

2021

Low Growth Hormone Levels Predict Poor Outcome of Hepatitis B Virus-Related Acute-on-Chronic Liver Failure.

Applications

Unspecified application

Species

Unspecified reactive species

Daxian Wu,Lingjian Zhang,Shanshan Ma,Yalei Zhao,Ronggao Chen,Fen Zhang,Qiuhong Liu,Xiaowei Xu,Zhongyang Xie

Clinical nutrition (Edinburgh, Scotland) 39:2778-2785 PubMed31883943

2019

Plasma inflammatory biomarkers and modifiable lifestyle factors associated with colorectal cancer risk.

Applications

Unspecified application

Species

Unspecified reactive species

Jimi Kim,Jeonghee Lee,Jae Hwan Oh,Hee Jin Chang,Dae Kyung Sohn,Aesun Shin,Jeongseon Kim

Oncology reports 42:1183-1193 PubMed31233204

2019

Synergistic antitumor effect of BRMS1 and sorafenib via inhibition of the PI3K/AKT/mTOR/ERK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jingyun Jin,Yonggang Guo,Yang Liu,Zhenguo Wang,Xiaosa Yang,Shengkai Sun

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human IGF1 ELISA Kit

ELISA - Human IGF1 ELISA Kit (AB211651)

Sandwich ELISA - Human IGF1 ELISA Kit (AB211651)

Sandwich ELISA - Human IGF1 ELISA Kit (AB211651)

Sandwich ELISA - Human IGF1 ELISA Kit (AB211651)

Sandwich ELISA - Human IGF1 ELISA Kit (AB211651)

Sandwich ELISA - Human IGF1 ELISA Kit (AB211651)

Sandwich ELISA - Human IGF1 ELISA Kit (AB211651)

Sandwich ELISA - Human IGF1 ELISA Kit (AB211651)

Sandwich ELISA - Human IGF1 ELISA Kit (AB211651)

Sandwich ELISA - Human IGF1 ELISA Kit (AB211651)

Sandwich ELISA - Human IGF1 ELISA Kit (AB211651)

Sandwich ELISA - Human IGF1 ELISA Kit (AB211651)

Sandwich ELISA - Human IGF1 ELISA Kit (AB211651)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Secondary Antibodies

Assay Kits

Primary Antibodies

Assay Kits

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

Product promise