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AB325900

Human IGF1 ELISA Kit, Chemiluminescent

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Human IGF1 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human IGF1 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 123.46 - 30000 pg/ml with a sensitivity of 49.7 pg/mL.
- Patent pending

View Alternative Names

IBP1, IGF-1, IGF1, Insulin-like growth factor 1, Insulin-like growth factor I, Mechano growth factor, Somatomedin-C, IGF-I, MGF

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Sandwich ELISA - Human IGF1 ELISA Kit, Chemiluminescent (AB325900)
  • sELISA

Lab

Sandwich ELISA - Human IGF1 ELISA Kit, Chemiluminescent (AB325900)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human IGF1 ELISA Kit, Chemiluminescent (AB325900)
  • sELISA

Lab

Sandwich ELISA - Human IGF1 ELISA Kit, Chemiluminescent (AB325900)

Example of human IGF1 standard curve in Sample Diluent NS + 1X Enhancer. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IGF1 ELISA Kit, Chemiluminescent (AB325900)
  • sELISA

Supplier Data

Sandwich ELISA - Human IGF1 ELISA Kit, Chemiluminescent (AB325900)

1% Serum from ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGF1 concentration was determined to be 78.06 pg/mL with a range of 45.41 – 141.07 pg/mL.

Sandwich ELISA - Human IGF1 ELISA Kit, Chemiluminescent (AB325900)
  • sELISA

Supplier Data

Sandwich ELISA - Human IGF1 ELISA Kit, Chemiluminescent (AB325900)

1% Serum from ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGF1 concentration was determined to be 129.12 ng/mL with a range of 69.09-184.22 ng/mL.

Sandwich ELISA - Human IGF1 ELISA Kit, Chemiluminescent (AB325900)
  • sELISA

Supplier Data

Sandwich ELISA - Human IGF1 ELISA Kit, Chemiluminescent (AB325900)

The concentrations of IGF1 were measured in duplicate, interpolated from the IGF1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 2.5%, plasma (citrate) 1.25%, plasma (EDTA) 1.25%, and plasma (heparin) 1.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGF1 concentration was determined to be 99.58 ng/mL in serum, 121.23 pg/mL in plasma (citrate), 103.65 ng/mL in plasma (EDTA), and 110.43 ng/mL in plasma (heparin).

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 49.7 pg/mL

Range

123.46 - 30000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human IGF1 ELISA Kit, Chemiluminescent (ab325900) is a rapid single-wash sandwich 55-min ELISA to measure Human IGF1 in Serum, Plasma – Citrate, Plasma – EDTA, Plasma – Heparin, Cell Culture Media with a sensitivity of 49.7 pg/mL.

How the assay works

Human IGF1 ELISA Kit, Chemiluminescent (ab325900) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human IGF1 (ab325900) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection
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Precision

[ { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.9" }, { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 9.5" } ]
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Recovery

[ { "sample": "Serum", "range": "83 - 90 %", "average": "= 86" }, { "sample": "Citrate plasma", "range": "97 - 104 %", "average": "= 100" }, { "sample": "EDTA Plasma", "range": "106 - 118 %", "average": "= 113" }, { "sample": "Heparin Plasma", "range": "91 - 111 %", "average": "= 100" }, { "sample": "Cell culture media", "range": "96 - 104 %", "average": "= 100" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation (PubMed : 21076856, PubMed : 24132240). Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb (By similarity). Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV : ITGB3 and ITGA6 : ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1 (PubMed : 19578119, PubMed : 22351760, PubMed : 23243309, PubMed : 23696648). As part of the MAPK/ERK signaling pathway, acts as a negative regulator of apoptosis in cardiomyocytes via promotion of STUB1/CHIP-mediated ubiquitination and degradation of ICER-type isoforms of CREM (By similarity).
See full target information IGF1
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websiteProtocolBooklet
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Product promise

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