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AB275102

Human IGF1 Receptor ELISA Kit

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Human IGF1 Receptor ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IGF1 Receptor with a sensitivity of 85 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD221, Insulin-like growth factor 1 receptor, Insulin-like growth factor I receptor, IGF-I receptor, IGF1R

5 Images
Sandwich ELISA - Human IGF1 Receptor ELISA Kit (AB275102)
  • sELISA

Supplier Data

Sandwich ELISA - Human IGF1 Receptor ELISA Kit (AB275102)

Interpolated concentrations of native IGF1 Receptor in human Hela (62.5 μg/mL), A431 (100 μg/mL and HepG2 (500 μg/mL) cell extracts.

The concentrations of IGF1 Receptor were measured in duplicate and interpolated from the IGF1 Receptor standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGF1 Receptor concentration was determined to be 3296 pg/mL in Hela cell extract, 16,503 pg/ml in A431 cell extract and 15,527 pg/mL in HepG2 cell extract.

Sandwich ELISA - Human IGF1 Receptor ELISA Kit (AB275102)
  • sELISA

Supplier Data

Sandwich ELISA - Human IGF1 Receptor ELISA Kit (AB275102)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGF1 Receptor concentration was determined to be 1937 pg/mL with a range of 1308 pg/ml - 3008 pg/mL.

Sandwich ELISA - Human IGF1 Receptor ELISA Kit (AB275102)
  • sELISA

Supplier Data

Sandwich ELISA - Human IGF1 Receptor ELISA Kit (AB275102)

Representative image of Human IGF1 Receptor ELISA Kit (ab275102)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human IGF1 Receptor Capture Antibody

- 10X Human IGF1 Receptor Detector Antibody

- Human IGF1 Receptor Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody diluent 5BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Sandwich ELISA - Human IGF1 Receptor ELISA Kit (AB275102)
  • sELISA

Supplier Data

Sandwich ELISA - Human IGF1 Receptor ELISA Kit (AB275102)

Interpolated concentrations of native IGF1 Receptor in human serum and plasma samples.

The concentrations of IGF1 Receptor were measured in duplicate, interpolated from the IGF1 Receptor standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100%, plasma (citrate) 100%, plasma (EDTA) 100% and plasma (heparin) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGF1 Receptor concentration was determined to be 1811 pg/mL in serum, 1172 pg/mL in plasma (citrate), 1594 pg/mL in plasma (EDTA) and 1575 pg/ml plasma (heparin).

Sandwich ELISA - Human IGF1 Receptor ELISA Kit (AB275102)
  • sELISA

Supplier Data

Sandwich ELISA - Human IGF1 Receptor ELISA Kit (AB275102)

Example of human IGF1 Receptor standard curve in Sample Diluent NS.

The IGF1 Receptor standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Serum, Cell Lysate, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 85 pg/mL

Range

312.5 - 20000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human IGF1 Receptor ELISA kit (ab275102) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human IGF1 Receptor protein in serum, plasma, and cell and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IGF1 Receptor with 85 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

IGF1 receptor (IGF -1 R) is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta subunits. This kit targets the alpha subunit. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. INS R and IGF-I R are receptor tyrosine kinases that consist of two ligand binding alpha subunits and two beta subunits. IGF-1 R Ligand binding induces autophosphorylation on multiple tyrosine residues of beta subunits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.5" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.7" } ]
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Recovery

[ { "sample": "Serum", "range": "87 - 119 %", "average": "= 104" }, { "sample": "EDTA Plasma", "range": "95 - 103 %", "average": "= 98" }, { "sample": "Heparin Plasma", "range": "90 - 101 %", "average": "= 96" }, { "sample": "Citrate plasma", "range": "85 - 90 %", "average": "= 87" }, { "sample": "Cell Lysate", "range": "98 - 107 %", "average": "= 103" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab275102-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB275102-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB275102-CMP11", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB275102-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB275102-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB275102-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB275102-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IGF1 Receptor Lyophilized Recombinant Protein", "number":"AB275102-CMP12", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IGF1 Receptor Capture Antibody", "number":"AB275102-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB275102-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IGF1 Receptor Detector Antibody", "number":"AB275102-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB275102-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB275102-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The IGF1 Receptor often referred to as IGF-1R is a transmembrane receptor protein composed of alpha and beta subunits. Studies indicate that the IGF1R protein is involved in mediating the effects of insulin-like growth factor 1 (IGF-1) and plays an important role in cellular signaling. It has a molecular weight of approximately 180 kDa and is widely expressed in various tissues with high concentrations in the liver muscle and brain. Being a tyrosine kinase receptor IGF1R plays an essential role in growth and metabolism.
Biological function summary

IGF1R contributes to several cellular processes including cell proliferation differentiation and survival. This receptor forms a complex upon ligand binding and undergoes autophosphorylation to activate intracellular signaling cascades. IGF1R activation recruits and phosphorylates insulin receptor substrates enabling the downstream signaling pathways that promote cell growth and survival. Its function in cellular responses makes it a focal point in understanding cell biology.

Pathways

IGF1R holds a central position in the PI3K/AKT and MAPK signaling cascades. These pathways play significant roles in cellular growth proliferation and survival. IGF1R phosphorylates various downstream effectors such as IRS-1 and Shc linking it to the activation of AKT and ERK respectively. Therefore it shares pathways with related proteins like the insulin receptor highlighting its importance in mediating similar biological responses.

IGF1R has been implicated in cancers and diabetes. Overexpression of IGF1R has been observed in various malignancies including breast cancer where it relates to resistance in treatment and poor prognosis with cell lines like MCF-7 often being studied in this context. Additionally disruptions in IGF1R signaling link to insulin resistance an important feature of type 2 diabetes. These connections make IGF1R a potential therapeutic target with IGF1R inhibitors currently under exploration to address these health challenges.
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Product protocols

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Target data

Receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). Binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survival of malignant cell. Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of multiple substrates, that function as signaling adapter proteins including, the insulin-receptor substrates (IRS1/2), Shc and 14-3-3 proteins. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways : the PI3K-AKT/PKB pathway and the Ras-MAPK pathway. The result of activating the MAPK pathway is increased cellular proliferation, whereas activating the PI3K pathway inhibits apoptosis and stimulates protein synthesis. Phosphorylated IRS1 can activate the 85 kDa regulatory subunit of PI3K (PIK3R1), leading to activation of several downstream substrates, including protein AKT/PKB. AKT phosphorylation, in turn, enhances protein synthesis through mTOR activation and triggers the antiapoptotic effects of IGFIR through phosphorylation and inactivation of BAD. In parallel to PI3K-driven signaling, recruitment of Grb2/SOS by phosphorylated IRS1 or Shc leads to recruitment of Ras and activation of the ras-MAPK pathway. In addition to these two main signaling pathways IGF1R signals also through the Janus kinase/signal transducer and activator of transcription pathway (JAK/STAT). Phosphorylation of JAK proteins can lead to phosphorylation/activation of signal transducers and activators of transcription (STAT) proteins. In particular activation of STAT3, may be essential for the transforming activity of IGF1R. The JAK/STAT pathway activates gene transcription and may be responsible for the transforming activity. JNK kinases can also be activated by the IGF1R. IGF1 exerts inhibiting activities on JNK activation via phosphorylation and inhibition of MAP3K5/ASK1, which is able to directly associate with the IGF1R.. When present in a hybrid receptor with INSR, binds IGF1. PubMed : 12138094 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, PubMed : 16831875 shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin.
See full target information IGF1R
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Obesity (Silver Spring, Md.) 31:2689-2698 PubMed37840435

2023

Lower muscle protein synthesis in humans with obesity concurrent with lower expression of muscle IGF1 splice variants.

Applications

Unspecified application

Species

Unspecified reactive species

Eduardo D S Freitas,Katon A Kras,Lori R Roust,Elena A De Filippis,Scot R Kimball,Matthew Buras,Christos S Katsanos
View all publications
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