AB324327

Human IGF2R (M6PR) ELISA Kit

SimpleStep
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Human IGF2R (M6PR) ELISA Kit is a Sandwich ELISA for the measurement of Human IGF2R (M6PR) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD222, MPRI, IGF2R, Cation-independent mannose-6-phosphate receptor, CI Man-6-P receptor, CI-MPR, M6PR, 300 kDa mannose 6-phosphate receptor, Insulin-like growth factor 2 receptor, Insulin-like growth factor II receptor, M6P/IGF2 receptor, MPR 300, IGF-II receptor, M6P/IGF2R

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Sandwich ELISA - Human IGF2R (M6PR) ELISA Kit (AB324327)

sELISA

Supplier Data

Sandwich ELISA - Human IGF2R (M6PR) ELISA Kit (AB324327)

Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean IGF2R concentration was determined to be 42.8 ng/mL with a range of 27.1 – 66.4 ng/mL.

sELISA

Supplier Data

Sandwich ELISA - Human IGF2R (M6PR) ELISA Kit (AB324327)

50 ng/mL each of recombinant human IGF2 was tested for specificity, and for interference in combination with 2,500 pg/mL of recombinant human IGF2R. The interpolated values, expressed in % of input (for specificity) or in % of human IGF2R control (for interference), are plotted (mean +/- SD, n =2).

sELISA

Supplier Data

Sandwich ELISA - Human IGF2R (M6PR) ELISA Kit (AB324327)

Example of human IGF2R standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human IGF2R (M6PR) ELISA Kit (AB324327)

Interpolated concentration of native IGF2R was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 20% human plasma (EDTA). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human IGF2R (M6PR) ELISA Kit (AB324327)

Example of human IGF2R standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human IGF2R (M6PR) ELISA Kit (AB324327)

Interpolated concentration of native IGF2R was measured in duplicate at different sample concentrations. Undiluted samples are 20% serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 64.904 pg/mL

Range

156.25 - 10000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human IGF2R (M6PR) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IGF2R (M6PR) protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture media. Quantitate Human IGF2R (M6PR) with 64.904 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.1" }, { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 1.4" } ]

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Recovery

[ { "sample": "Serum", "range": "102 - 118 %", "average": "= 108" }, { "sample": "Citrate plasma", "range": "93 - 102 %", "average": "= 97" }, { "sample": "EDTA Plasma", "range": "96 - 103 %", "average": "= 99" }, { "sample": "Heparin Plasma", "range": "106 - 124 %", "average": "= 113" }, { "sample": "Cell culture media", "range": "97 - 103 %", "average": "= 101" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Mediates the transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes (PubMed : 18817523, PubMed : 2963003). Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelysosomal compartment where the low pH mediates the dissociation of the complex (PubMed : 18817523, PubMed : 2963003). The receptor is then recycled back to the Golgi for another round of trafficking through its binding to the retromer (PubMed : 18817523). This receptor also binds IGF2 (PubMed : 18046459). Acts as a positive regulator of T-cell coactivation by binding DPP4 (PubMed : 10900005).

See full target information IGF2R

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websiteProtocolBooklet

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Product promise

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