JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB100540

Human IGFBP2 ELISA Kit

Be the first to review this product! Submit a review

|

(7 Publications)

Human IGFBP2 ELISA Kit is a Sandwich ELISA for the measurement of Human IGFBP2 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

BP2, IBP2, IGFBP2, Insulin-like growth factor-binding protein 2, IBP-2, IGF-binding protein 2, IGFBP-2

2 Images
Sandwich ELISA - Human IGFBP2 ELISA Kit (AB100540)
  • sELISA

Unknown

Sandwich ELISA - Human IGFBP2 ELISA Kit (AB100540)

Representative standard curve using ab100540

Sandwich ELISA - Human IGFBP2 ELISA Kit (AB100540)
  • sELISA

Unknown

Sandwich ELISA - Human IGFBP2 ELISA Kit (AB100540)

Representative standard curve using ab100540

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 20 pg/mL

Range

8.23 - 6000 pg/mL

Assay Platform

Microplate

style
left-column

Complementary Products

Assay Kits

AB260064

Human C-Peptide ELISA Kit

Recombinant|SimpleStep

ELISA - Human C-Peptide ELISA Kit (AB260064)

  • 0
  • 0
Assay Kits

AB211652

Human IGFBP3 ELISA Kit

Recombinant|SimpleStep

ELISA - Human IGFBP3 ELISA Kit (AB211652)

  • 0
  • 1
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Assay Kits

AB226901

Human Prolactin/PRL ELISA Kit

Recombinant|SimpleStep

ELISA - Human Prolactin/PRL ELISA Kit (AB226901)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Abcam's IGFBP2 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IGFBP2 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human IGFBP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and IGFBP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IGFBP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGFBP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples

style
left-column

Recovery

[ { "sample": "Cell culture supernatant", "range": "86 - 105 %", "average": "= 100.25" }, { "sample": "Serum", "range": "84 - 103 %", "average": "= 95.77" }, { "sample": "Plasma", "range": "85 - 104 %", "average": "= 97.59" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100540-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100540-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100540-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human IGFBP2", "number":"AB100540-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human IGFBP2 Standard (lyophilized)", "number":"AB100540-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100540-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin Concentrate", "number":"AB100540-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IGFBP2 Microplate (12 x 8 wells)", "number":"AB100540-CMP06", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100540-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100540-CMP08", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

Multifunctional protein that plays a critical role in regulating the availability of IGFs such as IGF1 and IGF2 to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner (PubMed : 18563800, PubMed : 38796567). Functions coordinately with receptor protein tyrosine phosphatase beta/PTPRB and the IGF1 receptor to regulate IGF1-mediated signaling by stimulating the phosphorylation of PTEN leading to its inactivation and AKT1 activation (PubMed : 22869525). Plays a positive role in cell migration via interaction with integrin alpha5/ITGA5 through an RGD motif (PubMed : 16569642). Additionally, interaction with ITGA5/ITGB1 enhances the adhesion of endothelial progenitor cells to endothelial cells (PubMed : 26076738). Upon mitochondrial damage, facilitates apoptosis with ITGA5 of podocytes, and then activates the phosphorylation of focal adhesion kinase (FAK)-mediated mitochondrial injury (PubMed : 38796567).
See full target information IGFBP2
style
left-column

Publications (7)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed38673963

2024

Transcriptomic Analysis of Extracellular Vesicles in the Search for Novel Plasma and Thrombus Biomarkers of Ischemic Stroke Etiologies.

Applications

Unspecified application

Species

Unspecified reactive species

Florencio J D M Machado,Juan Marta-Enguita,Susan U Gómez,Jose A Rodriguez,José Antonio Páramo-Fernández,María Herrera,Beatriz Zandio,Nuria Aymerich,Roberto Muñoz,Rebeca Bermejo,Javier Marta-Moreno,Begoña López,Arantxa González,Carmen Roncal,Josune Orbe

Molecular cancer 22:206 PubMed38093346

2023

Dynamic altruistic cooperation within breast tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Muhammad Sufyan Bin Masroni,Kee Wah Lee,Victor Kwan Min Lee,Siok Bian Ng,Chao Teng Law,Kok Siong Poon,Bernett Teck-Kwong Lee,Zhehao Liu,Yuen Peng Tan,Wee Ling Chng,Steven Tucker,Lynette Su-Mien Ngo,George Wai Cheong Yip,Min En Nga,Susan Swee Shan Hue,Thomas Choudary Putti,Boon Huat Bay,Qingsong Lin,Lihan Zhou,Mikael Hartman,Tze Ping Loh,Manikandan Lakshmanan,Sook Yee Lee,Vinay Tergaonkar,Huiwen Chua,Adeline Voon Hui Lee,Eric Yew Meng Yeo,Mo-Huang Li,Chan Fong Chang,Zizheng Kee,Karen Mei-Ling Tan,Soo Yong Tan,Evelyn Siew-Chuan Koay,Marco Archetti,Sai Mun Leong

Diabetes, metabolic syndrome and obesity : targets and therapy 15:3429-3436 PubMed36353668

2022

Relationship of Plasma Gremlin 1 Levels with Body Adiposity and Glycemic Control in Saudi Female Type 2 Diabetes Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Khalid A Al-Regaiey,Syed Shahid Habib,Ahmed R Alshamasi,Abdullah F Alnuwaybit,Bader A Alwhaibi,Naif M Alsulais,Abdullah I Alothman,Faisal M Alomar,Muhammad Iqbal

BMC gastroenterology 20:199 PubMed32586279

2020

Effects of gastric sleeve surgery on the serum levels of GH, IGF-1 and IGF-binding protein 2 in healthy obese patients.

Applications

Unspecified application

Species

Unspecified reactive species

Khalid Al-Regaiey,Suad Alshubrami,Ibrahim Al-Beeshi,Torki Alnasser,Abdulnasser Alwabel,Hassan Al-Beladi,Omar Al-Tujjar,Abdulrahman Alnasser,Assim A Alfadda,Muhammad Iqbal

Experimental gerontology 58:154-8 PubMed25106097

2014

Protein molecular forms of insulin-like growth factor binding protein-2 change with aging.

Applications

Unspecified application

Species

Unspecified reactive species

Miloš Sunderić,Nevena Mihailović,Olgica Nedić

Frontiers in aging neuroscience 6:191 PubMed25136316

2014

The generation of induced pluripotent stem cells for macular degeneration as a drug screening platform: identification of curcumin as a protective agent for retinal pigment epithelial cells against oxidative stress.

Applications

sELISA

Species

Human

Yun-Ching Chang,Wei-Chao Chang,Kuo-Hsuan Hung,Der-Ming Yang,Yung-Hsin Cheng,Yi-Wen Liao,Lin-Chung Woung,Ching-Yao Tsai,Chih-Chien Hsu,Tai-Chi Lin,Jorn-Hon Liu,Shih-Hwa Chiou,Chi-Hsien Peng,Shih-Jen Chen

Free radical biology & medicine 65:1195-1200 PubMed24051179

2013

Detection and identification of oxidized insulin-like growth factor-binding proteins and receptors in patients with colorectal carcinoma.

Applications

sELISA

Species

Human

Olgica Nedić,Dragana Robajac,Miloš Šunderić,Goran Miljuš,Blagoje Đukanović,Vesna Malenković
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com