Human IGFBP2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IGFBP2 with a sensitivity of 115.38 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
BP2, IBP2, IGFBP2, Insulin-like growth factor-binding protein 2, IBP-2, IGF-binding protein 2, IGFBP-2
- ELISA
Supplier Data
ELISA - Human IGFBP2 ELISA Kit (AB272207)
- sELISA
Supplier Data
Sandwich ELISA - Human IGFBP2 ELISA Kit (AB272207)
Example of human IGFBP2 standard curve in Sample Diluent NS.
The IGFBP2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human IGFBP2 ELISA Kit (AB272207)
Interpolated concentrations of native IGFBP2 in human PBMC cell culture supernatant samples treated for 46 hours with 1.5% PHA-M.
The concentrations of IGFBP2 were measured in duplicates, interpolated from the IGFBP2 standard curves and corrected for sample dilution. Undiluted samples are as follows : PBMC supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP2 concentration was determined to be 3,034.42 pg/mL in PBMC supernatant.
- sELISA
Supplier Data
Sandwich ELISA - Human IGFBP2 ELISA Kit (AB272207)
Serum from ten individual healthy human male donors was measured in duplicate
- sELISA
Supplier Data
Sandwich ELISA - Human IGFBP2 ELISA Kit (AB272207)
Interpolated concentrations of native IGFBP2 in human serum and plasma samples.
The concentrations of IGFBP2 were measured in duplicates, interpolated from the IGFBP2 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 5%, plasma (citrate) 5%, plasma (heparin) 10%, and plasma (EDTA) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP2 concentration was determined to be 257.87 ng/mL in serum, 273.81 ng/mL in plasma (citrate), 139.86 ng/ml in plasma (heparin), and 118.43 ng/ml in plasma (EDTA).
- sELISA
Supplier Data
Sandwich ELISA - Human IGFBP2 ELISA Kit (AB272207)
Human IGFBP2 competitor curve comparison.
Standard Curve comparison between Human IGBPF2 SimpleStep ELISA kit and traditional ELISA kit from leading competitor.
- sELISA
Supplier Data
Sandwich ELISA - Human IGFBP2 ELISA Kit (AB272207)
Human IGFBP2 standard curve comparison.
Standard Curve comparison between the original Human IGFBP2 SimpleStep ELISA kit (ab215082) and current Human MMP3 SimpleStep ELISA kit (ab272207).
Complementary Products
Reactivity data
Product details
Human IGFBP2 ELISA Kit ab272207 is a rapid single-wash 90-min Sandwich ELISA to measure Human IGFBP2 in cell culture supernatant, EDTA plasma, heparin plasma, serum, urine. This SimpleStep sensitivity is 115.38 pg/mL.
How the assay works
Human IGFBP2 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human IGFBP2 ELISA Kit ab272207 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-IGFBP2 antibody [EPR21134-8] - BSA and Azide free (Capture) ab272413
- Anti-IGFBP2 antibody [EPR21134-7] - BSA and Azide free (Detector) ab272413
Insulin-like growth factor-binding protein 2 (IGFBP2) is an IGF binding protein involved in prolonging its half-life and has both inhibitory and stimulatory effects on IGFs. IGFBP2 can also alter the interaction of IGFs with their receptors.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Product protocols
- Visit the General protocols
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Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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