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AB100541

Human IGFBP3 ELISA Kit

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(2 Publications)

Human IGFBP3 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human IGFBP3 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

IBP3, IGFBP3, Insulin-like growth factor-binding protein 3, IBP-3, IGF-binding protein 3, IGFBP-3

1 Images
Sandwich ELISA - Human IGFBP3 ELISA Kit (AB100541)
  • sELISA

Unknown

Sandwich ELISA - Human IGFBP3 ELISA Kit (AB100541)

Representative standard curve using ab100541

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 80 pg/mL

Range

74.07 - 18000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's IGFBP3 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IGFBP3 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human IGFBP3 coated on a 96-well plate. Standards and samples are pipetted into the wells and IGFBP3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IGFBP3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGFBP3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples

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Recovery

[ { "sample": "Cell culture supernatant", "range": "86 - 107 %", "average": "= 97.46" }, { "sample": "Serum", "range": "85 - 105 %", "average": "= 97.46" }, { "sample": "Plasma", "range": "84 - 106 %", "average": "= 96.69" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100541-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100541-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100541-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human IGFBP3", "number":"AB100541-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human IGFBP3 Standard (lyophilized)", "number":"AB100541-CMP07", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100541-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IGFBP3 Microplate (12 x 8 wells)", "number":"AB100541-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"320X HRP-Streptavidin Concentrate", "number":"AB100541-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100541-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100541-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Multifunctional protein that plays a critical role in regulating the availability of IGFs such as IGF1 and IGF2 to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner (PubMed : 10874028, PubMed : 19556345). Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R (PubMed : 20353938). Inhibits the positive effect of humanin on insulin sensitivity (PubMed : 19623253). Promotes testicular germ cell apoptosis (PubMed : 19952275). Acts via LRP-1/alpha2M receptor, also known as TGF-beta type V receptor, to mediate cell growth inhibition independent of IGF1 (PubMed : 9252371). Mechanistically, induces serine-specific dephosphorylation of IRS1 or IRS2 upon ligation to its receptor, leading to the inhibitory cascade (PubMed : 15371331). In the nucleus, interacts with transcription factors such as retinoid X receptor-alpha/RXRA to regulate transcriptional signaling and apoptosis (PubMed : 10874028).
See full target information IGFBP3
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

European journal of nutrition 59:2497-2506 PubMed31570976

2019

Maternal high-protein diet modulates hepatic growth axis in weaning piglets by reprogramming the IGFBP-3 gene.

Applications

Unspecified application

Species

Unspecified reactive species

Rihua Cong,Xiaoli Qu,Hui Zhang,Yongling Hu,Silin Ye,Demin Cai,Xian Li,Hao-Yu Liu

Free radical biology & medicine 65:1195-1200 PubMed24051179

2013

Detection and identification of oxidized insulin-like growth factor-binding proteins and receptors in patients with colorectal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Olgica Nedić,Dragana Robajac,Miloš Šunderić,Goran Miljuš,Blagoje Đukanović,Vesna Malenković
View all publications
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