Human IGFBP3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IGFBP3 with a sensitivity of 86.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations
View Alternative Names
IBP3, IGFBP3, Insulin-like growth factor-binding protein 3, IBP-3, IGF-binding protein 3, IGFBP-3
- ELISA
Supplier Data
ELISA - Human IGFBP3 ELISA Kit (AB211652)
- sELISA
Unknown
Sandwich ELISA - Human IGFBP3 ELISA Kit (AB211652)
Human IGFBP3 standard curve comparison data.
Standard curve comparison between human IGFBP3 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.
- sELISA
Unknown
Sandwich ELISA - Human IGFBP3 ELISA Kit (AB211652)
Interpolated concentrations of native IGFBP3 in human serum and plasma samples.
The concentrations of IGFBP3 were measured in duplicates, interpolated from the IGFBP3 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 300, plasma (citrate) 1 : 300, plasma (heparin) 1 : 333, and plasma (EDTA) 1 : 500. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP3 concentration was determined to be 2,161 ng/mL in serum, 2,175 ng/mL in plasma (citrate), 822 ng/mL in plasma (heparin), and 1,944 ng/mL in plasma (EDTA).
- sELISA
Supplier Data
Sandwich ELISA - Human IGFBP3 ELISA Kit (AB211652)
Serum from ten individual healthy human male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP3 concentration was determined to be 1442 ng/mL with a range of 104 – 3,095 ng/mL.
- sELISA
Unknown
Sandwich ELISA - Human IGFBP3 ELISA Kit (AB211652)
Interpolated concentrations of native IGFBP3 in SH-SY5Y supernatant samples.
The concentrations of IGFBP3 were measured in duplicates, interpolated from the IGFBP3 standard curves and corrected for sample dilution. Undiluted samples are as follows : SH-SY5Y supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP3 concentration was determined to be 4.28 ng/ml in SH-SY5Y supernatant.
- sELISA
Supplier Data
Sandwich ELISA - Human IGFBP3 ELISA Kit (AB211652)
Example of human IGFBP3 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
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Reactivity data
Product details
Human IGFBP3 ELISA Kit ab211652 is a rapid single-wash 90-min Sandwich ELISA to measure Human IGFBP3 in cell culture supernatant, plasma, serum. This SimpleStep sensitivity is 86.6 pg/mL.
How the assay works
Human IGFBP3 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human IGFBP3 ELISA Kit ab211652 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-IGFBP3 antibody [EPR18680-153] - BSA and Azide free (Capture) ab242809
- Anti-IGFBP3 antibody [EPR18680-118] - BSA and Azide free (Detector) ab242809
Insulin like growth factor binding protein 3 (IGFBP3) belongs to the family of insulin like growth factor (IGF) binding proteins. IGFBP3 is the main IGF binding protein in plasma, and forms a complex with IGF-I or IGF-II and an acid-labile subunit. IGFBP3 is expressed in multiple tissues, where together with IGFs, they play important roles in glucose homeostasis, cell survival, cell growth and differentiation. The concentrations of IGFBP3 are altered in disease states like type I diabetes, where urinary levels are increased during microalbuminuria, and in prostate cancer, where IGFBP3 protein levels decrease.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
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Publications (5)
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International journal of molecular sciences 24: PubMed37175778
2023
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Scientific reports 12:11503 PubMed35798794
2022
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Prostate cancer and prostatic diseases 25:86-92 PubMed35152272
2022
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Genetics in medicine : official journal of the American College of Medical Genetics 23:1664-1672 PubMed34040195
2021
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Journal of proteomics 152:321-328 PubMed27890796
2016
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Product promise
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