Human IGFBP3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IGFBP3 with a sensitivity of 86.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations
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- International journal of molecular sciences 24:2023Intraocular Pressure-Lowering and Retina-Protective Effects of Exosome-Rich Conditioned Media from Human Amniotic Membrane Stem Cells in a Rat Model of Glaucoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHye-Rim Seong,Chan Ho Noh,Sangryong Park,Sumin Cho,Seok-Jin Hong,Ah-Young Lee,Dongho Geum,Soon-Cheol Hong,Dongsun Park,Tae Myoung Kim,Ehn-Kyoung Choi,Yun-Bae KimPubMed 37175778
- Scientific reports 12:115032022Roles of IGFBP-3 in cell migration and growth in an endophytic tongue squamous cell carcinoma cell line.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEsther Feng Ying Ng,Atsushi Kaida,Hitomi Nojima,Masahiko MiuraPubMed 35798794
- Prostate cancer and prostatic diseases 25:86-922022Exercise in advanced prostate cancer elevates myokine levels and suppresses in-vitro cell growth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJin-Soo Kim,Dennis R Taaffe,Daniel A Galvão,Nicolas H Hart,Elin Gray,Charles J Ryan,Stacey A Kenfield,Fred Saad,Robert U NewtonPubMed 35152272
- Genetics in medicine : official journal of the American College of Medical Genetics 23:1664-16722021SNORD116 and growth hormone therapy impact IGFBP7 in Prader-Willi syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSanaa Eddiry,Gwenaelle Diene,Catherine Molinas,Juliette Salles,Françoise Conte Auriol,Isabelle Gennero,Eric Bieth,Boris V Skryabin,Timofey S Rozhdestvensky,Lisa C Burnett,Rudolph L Leibel,Maithé Tauber,Jean Pierre SallesPubMed 34040195
- Journal of proteomics 152:321-3282016Temporal profiles of plasma proteome during childhood development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChih-Wei Liu,Lisa Bramer,Bobbie-Jo Webb-Robertson,Kathleen Waugh,Marian J Rewers,Qibin ZhangPubMed 27890796
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 122 - 7800 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 86.6 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Multifunctional protein that plays a critical role in regulating the availability of IGFs such as IGF1 and IGF2 to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner (PubMed:10874028, PubMed:19556345). Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R (PubMed:20353938). Inhibits the positive effect of humanin on insulin sensitivity (PubMed:19623253). Promotes testicular germ cell apoptosis (PubMed:19952275). Acts via LRP-1/alpha2M receptor, also known as TGF-beta type V receptor, to mediate cell growth inhibition independent of IGF1 (PubMed:9252371). Mechanistically, induces serine-specific dephosphorylation of IRS1 or IRS2 upon ligation to its receptor, leading to the inhibitory cascade (PubMed:15371331). In the nucleus, interacts with transcription factors such as retinoid X receptor-alpha/RXRA to regulate transcriptional signaling and apoptosis (PubMed:10874028).
Alternative names
IBP3, IGFBP3, Insulin-like growth factor-binding protein 3, IBP-3, IGF-binding protein 3, IGFBP-3
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Recommended products
Human IGFBP3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IGFBP3 with a sensitivity of 86.6 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 122 - 7800 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 86.6 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. 3.1 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 6.5 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 100 | Range 97 - 103 % |
Sample type Serum | Average % = 110 | Range 107 - 114 % |
Sample type Citrate plasma | Average % = 104 | Range 98 - 109 % |
Sample type EDTA Plasma | Average % = 104 | Range 104 - 104 % |
Sample type Heparin Plasma | Average % = 96 | Range 85 - 106 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human IGFBP3 ELISA Kit ab211652 is a rapid single-wash 90-min Sandwich ELISA to measure Human IGFBP3 in cell culture supernatant, plasma, serum. This SimpleStep sensitivity is 86.6 pg/mL.
How the assay works
Human IGFBP3 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human IGFBP3 ELISA Kit ab211652 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-IGFBP3 antibody [EPR18680-153] - BSA and Azide free (Capture) Anti-IGFBP3 antibody [EPR18680-118] - BSA and Azide free (Detector) ab242809
- Anti-IGFBP3 antibody [EPR18680-118] - BSA and Azide free (Detector) Anti-IGFBP3 antibody [EPR18680-118] - BSA and Azide free (Detector) ab242809
Insulin like growth factor binding protein 3 (IGFBP3) belongs to the family of insulin like growth factor (IGF) binding proteins. IGFBP3 is the main IGF binding protein in plasma, and forms a complex with IGF-I or IGF-II and an acid-labile subunit. IGFBP3 is expressed in multiple tissues, where together with IGFs, they play important roles in glucose homeostasis, cell survival, cell growth and differentiation. The concentrations of IGFBP3 are altered in disease states like type I diabetes, where urinary levels are increased during microalbuminuria, and in prostate cancer, where IGFBP3 protein levels decrease.
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6 product images
ELISA - Human IGFBP3 ELISA Kit (ab211652)
Sandwich ELISA - Human IGFBP3 ELISA Kit (ab211652)
Human IGFBP3 standard curve comparison data.
Standard curve comparison between human IGFBP3 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.
Sandwich ELISA - Human IGFBP3 ELISA Kit (ab211652)
Example of human IGFBP3 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human IGFBP3 ELISA Kit (ab211652)
Interpolated concentrations of native IGFBP3 in human serum and plasma samples.
The concentrations of IGFBP3 were measured in duplicates, interpolated from the IGFBP3 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:300, plasma (citrate) 1:300, plasma (heparin) 1:333, and plasma (EDTA) 1:500. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP3 concentration was determined to be 2,161 ng/mL in serum, 2,175 ng/mL in plasma (citrate), 822 ng/mL in plasma (heparin), and 1,944 ng/mL in plasma (EDTA).
Sandwich ELISA - Human IGFBP3 ELISA Kit (ab211652)
Interpolated concentrations of native IGFBP3 in SH-SY5Y supernatant samples.
The concentrations of IGFBP3 were measured in duplicates, interpolated from the IGFBP3 standard curves and corrected for sample dilution. Undiluted samples are as follows: SH-SY5Y supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP3 concentration was determined to be 4.28 ng/ml in SH-SY5Y supernatant.
Sandwich ELISA - Human IGFBP3 ELISA Kit (ab211652)
Serum from ten individual healthy human male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP3 concentration was determined to be 1442 ng/mL with a range of 104 – 3,095 ng/mL.
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