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AB326904

Human IGFBP3 ELISA Kit, Chemiluminescent

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Human IGFBP3 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human IGFBP3 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 58.35 - 14180 pg/ml with a sensitivity of 43.7 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

View Alternative Names

IBP3, IGFBP3, Insulin-like growth factor-binding protein 3, IBP-3, IGF-binding protein 3, IGFBP-3

4 Images
Sandwich ELISA - Human IGFBP3 ELISA Kit, Chemiluminescent (AB326904)
  • sELISA

Unknown

Sandwich ELISA - Human IGFBP3 ELISA Kit, Chemiluminescent (AB326904)

Interpolated concentrations of native IGFBP3 in human serum and plasma samples.

The concentrations of IGFBP3 were measured in duplicates, interpolated from the IGFBP3 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 300, plasma (citrate) 1 : 300, plasma (heparin) 1 : 333, and plasma (EDTA) 1 : 500. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP3 concentration was determined to be 2,161 ng/mL in serum, 2,175 ng/mL in plasma (citrate), 822 ng/mL in plasma (heparin), and 1,944 ng/mL in plasma (EDTA).

Sandwich ELISA - Human IGFBP3 ELISA Kit, Chemiluminescent (AB326904)
  • sELISA

Unknown

Sandwich ELISA - Human IGFBP3 ELISA Kit, Chemiluminescent (AB326904)

Interpolated concentrations of native IGFBP3 in SH-SY5Y supernatant samples.

The concentrations of IGFBP3 were measured in duplicates, interpolated from the IGFBP3 standard curves and corrected for sample dilution. Undiluted samples are as follows : SH-SY5Y supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP3 concentration was determined to be 4.28 ng/ml in SH-SY5Y supernatant.

Sandwich ELISA - Human IGFBP3 ELISA Kit, Chemiluminescent (AB326904)
  • sELISA

Supplier Data

Sandwich ELISA - Human IGFBP3 ELISA Kit, Chemiluminescent (AB326904)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP3 concentration was determined to be 1442 ng/mL with a range of 104 – 3,095 ng/mL.

Sandwich ELISA - Human IGFBP3 ELISA Kit, Chemiluminescent (AB326904)
  • sELISA

Lab

Sandwich ELISA - Human IGFBP3 ELISA Kit, Chemiluminescent (AB326904)

Standard curve comparison between the Chemiluminescent Human IGFBP3 SimpleStep ELISA and colorimetric Human IGFBP3 SimpleStep ELISA (ab211652) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Luminescent

Sample types

Cell culture supernatant, Serum, Citrate plasma, EDTA Plasma, Heparin Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 43.7 pg/mL

Range

58.35 - 14180 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human IGFBP3 ELISA Kit, Chemiluminescent (ab326904) is a rapid single-wash sandwich 55-min ELISA to measure Human IGFBP3 in Cell culture supernatant, Serum, Citrate plasma, EDTA Plasma, Heparin Plasma with a sensitivity of 43.7 pg/mL.

How the assay works

Human IGFBP3 ELISA Kit, Chemiluminescent (ab326904) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human IGFBP3 (ab326904) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-IGFBP3 antibody [EPR18680-153] - BSA and Aside free (Capture) ab242560
- Anti-IGFBP3 antibody [EPR18680-118_1] - BSA and Aside free (Detector) ab242809
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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.1" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.5" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "97 - 103 %", "average": "= 103" }, { "sample": "Serum", "range": "107 - 114 %", "average": "= 114" }, { "sample": "Citrate plasma", "range": "98 - 109 %", "average": "= 109" }, { "sample": "EDTA Plasma", "range": "104 - 104 %", "average": "= 104" }, { "sample": "Heparin Plasma", "range": "85 - 106 %", "average": "= 106" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab326904-1x96Tests", "assetComponentDetails": [ { "size":"1 x 600 µL", "name":"10X Human IGFBP3 Detector Antibody", "number":"AB326904-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB326904-CMP10", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB326904-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB326904-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IGFBP3 Capture Antibody", "number":"AB326904-CMP01", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB326904-CMP04", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB326904-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB326904-CMP09", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB326904-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IGFBP3 Lyophilized Recombinant Protein", "number":"AB326904-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Multifunctional protein that plays a critical role in regulating the availability of IGFs such as IGF1 and IGF2 to their receptors and thereby regulates IGF-mediated cellular processes including proliferation, differentiation, and apoptosis in a cell-type specific manner (PubMed : 10874028, PubMed : 19556345). Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R (PubMed : 20353938). Inhibits the positive effect of humanin on insulin sensitivity (PubMed : 19623253). Promotes testicular germ cell apoptosis (PubMed : 19952275). Acts via LRP-1/alpha2M receptor, also known as TGF-beta type V receptor, to mediate cell growth inhibition independent of IGF1 (PubMed : 9252371). Mechanistically, induces serine-specific dephosphorylation of IRS1 or IRS2 upon ligation to its receptor, leading to the inhibitory cascade (PubMed : 15371331). In the nucleus, interacts with transcription factors such as retinoid X receptor-alpha/RXRA to regulate transcriptional signaling and apoptosis (PubMed : 10874028).
See full target information IGFBP3
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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