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AB273151

Human IgG ELISA kit - 30 minutes

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Human IgG ELISA kit - 30 minutes is a sandwich ELISA designed to quantify human IgG with a sensitivity of 4 ng/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Rapid and easy method - 30 min protocol
- Wide dynamic range - quantifies 16 - 1000 ng/mL

View Alternative Names

Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1

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ELISA - Human IgG ELISA kit - 30 minutes (AB273151)
  • ELISA

Unknown

ELISA - Human IgG ELISA kit - 30 minutes (AB273151)

Human Immunoglobulin G Quantification Standard curve.

Example standard curve.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Serum

Assay type

Quantitative

Sensitivity

= 4 ng/mL

Range

16 - 1000 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Human IgG quantification Kit (ab273151) provides a rapid and easy method (one step ELISA) for the quantitative determination of human IgG in cell culture supernatant and serum. The kit includes ready-to-use reagents necessary to analyze 89 samples in less than 30 minutes.

A polyclonal antibody specific to the gamma heavy chain of human IgG is pre-coated onto microwells. Samples and standards are pipetted into microwells and human IgG present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-human IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing microwells in order to remove any non specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of human IgG in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample H 1/1000", "replicates": 30, "mean": null, "standardDeviation": "2.4", "coefficientOfVariability": null }, { "reproducibilityType": "Inter", "sample": "Sample H 1/250", "replicates": 30, "mean": null, "standardDeviation": "7", "coefficientOfVariability": null }, { "reproducibilityType": "Inter", "sample": "Sample H 1/500", "replicates": 30, "mean": null, "standardDeviation": "4.6", "coefficientOfVariability": null }, { "reproducibilityType": "Intra", "sample": "G 1/100", "replicates": 10, "mean": "32.7 µg/mL", "standardDeviation": "8.84", "coefficientOfVariability": null }, { "reproducibilityType": "Intra", "sample": "Sample A 1/100", "replicates": 10, "mean": "6.8 µg/mL", "standardDeviation": "10.12", "coefficientOfVariability": null }, { "reproducibilityType": "Intra", "sample": "Sample B 1/100", "replicates": 10, "mean": "13.4 µg/mL", "standardDeviation": "6.13", "coefficientOfVariability": null }, { "reproducibilityType": "Intra", "sample": "Sample C 1/100", "replicates": 10, "mean": "13.9 µg/mL", "standardDeviation": "5.71", "coefficientOfVariability": null }, { "reproducibilityType": "Intra", "sample": "Sample D 1/100", "replicates": 10, "mean": "18.8 µg/mL", "standardDeviation": "5.19", "coefficientOfVariability": null }, { "reproducibilityType": "Intra", "sample": "Sample E 1/100", "replicates": 10, "mean": "23.5 µg/mL", "standardDeviation": "6.5", "coefficientOfVariability": null }, { "reproducibilityType": "Intra", "sample": "Sample F 1/100", "replicates": 10, "mean": "26.9 µg/mL", "standardDeviation": "5.42", "coefficientOfVariability": null } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268). Mediates IgG effector functions on monocytes triggering ADCC of virus-infected cells.
See full target information IGHG1
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websiteProtocolBooklet
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