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AB195215

Human IgG ELISA Kit

4

(6 Reviews)

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(37 Publications)

Human IgG ELISA kit is a single-wash, 90-minute SimpleStep ELISA® assay designed for the quantification of human Immunoglobulin G (IgG) with a sensitivity of 0.02 ng/mL. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.

- Colorimetric sandwich ELISA - 450nm readout; works on any plate reader
- High-throughput options – available in 96-well and 384-well formats, supporting large-scale sample processing.
- Broad sample compatibility – works with serum, plasma, cell culture supernatants, and other biological fluids
- Cited in over 20 publications

View Alternative Names

Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1

14 Images
Sandwich ELISA - Human IgG ELISA Kit (AB195215)
  • sELISA

Supplier Data

Sandwich ELISA - Human IgG ELISA Kit (AB195215)

IgG levels in individual healthy donors.

Ten individual healthy donors were evaluated for the presence of IgG in serum using this assay. Results were interpolated from the standard curve in Sample Diluent NS and corrected for sample dilution (1 : 5x106). The mean level of IgG was found at 12.5 mg/mL with a range of 9 – 18.4mg/mL.

ELISA - Human IgG ELISA Kit (AB195215)
  • ELISA

Unknown

ELISA - Human IgG ELISA Kit (AB195215)

IgG1-4 isotypes are detected by this kit.

Human IgG1, IgG2, IgG3 and IgG4 were tested at 5ng/mL

Sandwich ELISA - Human IgG ELISA Kit (AB195215)
  • sELISA

Unknown

Sandwich ELISA - Human IgG ELISA Kit (AB195215)

Linearity of dilution – native IgG in human serum, plasma (citrate, EDTA and Heparin), and culture media.

Native IgG was measured in the human serum, plasma citrate, plasma EDTA, plasma heparin and culture media in a 2-fold dilution series. Sample dilutions were made in Sample Diluent NS for all samples, purified IgG was spiked into culture media and diluted in a 2-fold dilution series in Sample Diluent NS

Sandwich ELISA - Human IgG ELISA Kit (AB195215)
  • sELISA

Unknown

Sandwich ELISA - Human IgG ELISA Kit (AB195215)

Raw data values for example of Human IgG standard curve in Sample Diluent NS.

Raw data values are shown in the table

Sandwich ELISA - Human IgG ELISA Kit (AB195215)
  • sELISA

Unknown

Sandwich ELISA - Human IgG ELISA Kit (AB195215)

Linearity of dilution – native IgG in human milk, urine, saliva and HLH extract.

Native IgG was measured in milk, saliva, urine and liver homogenate (HLH) in a 2-fold dilution series. Sample dilutions were made in Sample Diluent NS for all samples except for HLH, which was carried out in 1X cell extraction buffer PTR.

Sandwich ELISA - Human IgG ELISA Kit (AB195215)
  • sELISA

Supplier Data

Sandwich ELISA - Human IgG ELISA Kit (AB195215)

Comparison of IgG levels in Human milk, urine and saliva.

Bodily fluids from 3 different donors were evaluated for the presence of IgG using this assay. Results were interpolated from the standard curve in sample diluent NS and corrected for sample dilution (1 : 2.5x104). The mean levels in Milk were found at 20.7 μg/mL, in Urine at 0.8 μg/mL and in Saliva at 11.1 μg/mL.

Sandwich ELISA - Human IgG ELISA Kit (AB195215)
  • sELISA

Supplier Data

Sandwich ELISA - Human IgG ELISA Kit (AB195215)

Example of Human IgG standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IgG ELISA Kit (AB195215)
  • sELISA

Supplier Data

Sandwich ELISA - Human IgG ELISA Kit (AB195215)

Example of Human IgG standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IgG ELISA Kit (AB195215)
  • sELISA

Unknown

Sandwich ELISA - Human IgG ELISA Kit (AB195215)

Raw data values for example of Human IgG standard curve in 1X Cell Extraction Buffer PTR.

Raw data values are shown in the table

Sandwich ELISA - Human IgG ELISA Kit (AB195215)
  • sELISA

Lab

Sandwich ELISA - Human IgG ELISA Kit (AB195215)

Interpolated concentration of native IgG was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 1,000,000 human serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human IgG ELISA Kit (AB195215)
  • sELISA

Lab

Sandwich ELISA - Human IgG ELISA Kit (AB195215)

Example of Human IgG standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IgG ELISA Kit (AB195215)
  • sELISA

Lab

Sandwich ELISA - Human IgG ELISA Kit (AB195215)

Example of Human IgG standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IgG ELISA Kit (AB195215)
  • sELISA

Lab

Sandwich ELISA - Human IgG ELISA Kit (AB195215)

SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human IgG ELISA Kit (AB195215)
  • sELISA

Lab

Sandwich ELISA - Human IgG ELISA Kit (AB195215)

Interpolated concentration of native IgG was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 100 ng/mL human liver extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Key facts

Detection method

Colorimetric

Sample types

Tissue Lysate, Cerebral Spinal Fluid, Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.02 ng/mL

Range

0.23 - 15 ng/mL

Assay time

1h 30m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human IgG ELISA Kit ab195215 is a rapid single-wash 90-min ELISA kit to measure Human IgG in tissue lysate, cerebral spinal fluid, saliva, urine, cell culture supernatant, milk, serum and plasma. This SimpleStep ELISA® sensitivity is 0.02 ng/mL.

How the assay works

Human IgG SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Human IgM, human IgA and human IgE were prepared at 10 ng/mL and 250 ng/mL in Sample Diluent NS and assayed for cross reactivity. No cross-reactivity was observed for IgM or IgE at either concentration with a mean OD deviation from background of -0.01. No cross-reactivity was observed for IgA at 10 ng/mL and only 0.4% cross-reactivity at 250 ng/mL of IgA. Purified human IgG was assayed at 5 ng/mL in the presence and absence of 250 ng/mL of human IgM, human IgA and human IgE to determine interference. After background subtraction, recovery of human IgG was observed at a mean of 98% with a standard deviation of 0.06.

Human IgG ELISA Kit ab195215 protocol summary:

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Human IgG ELISA Kit ab195215

Human IgG ELISA Kit ab195215 has been used to study human immunoglobulin g in autoimmune diseases and infections in various physio-pathological contexts such as obesity(1)and SARS-CovV-2(2).

References:

(1)Leonid Kagan et al. 2023, PMID: 36949370,
(2)Peter D Sun et al. 2023, PMID: 37821447

Save your precious samples

Our 384-well Human IgG ELISA Kit only requires a maxmum of 12.5µL

Related and recommended products

Human IgG ELISA Kit ab195215 is commonly used to measure immune system functioning.

Other ELISA kits to measure Immunoglobulins include:

- Human IgM ELISA kit ab214568
- Human IgA ELISA kit ab196263
- Human IgD ELISA kit ab320794
- Human IgE ELISA kit ab195216
- Human IgG ELISA Kit (10 x 96 well plate) ab212169

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "14.7" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.4" } ]
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Recovery

[ { "sample": "Milk", "range": "83 - 94 %", "average": "= 89" }, { "sample": "Serum", "range": "88 - 125 %", "average": "= 101" }, { "sample": "EDTA Plasma", "range": "87 - 93 %", "average": "= 90.17" }, { "sample": "Cerebral Spinal Fluid", "range": "88 - 105 %", "average": "= 98" }, { "sample": "Urine", "range": "82 - 93 %", "average": "= 87" }, { "sample": "Saliva", "range": "80 - 106 %", "average": "= 89" }, { "sample": "Heparin Plasma", "range": "100 - 100 %", "average": "= 100" }, { "sample": "Citrate plasma", "range": "98 - 102 %", "average": "= 100" }, { "sample": "Cell culture media", "range": "96 - 115 %", "average": "= 107" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Immunoglobulin G (IgG) serves as an essential component of the immune system functioning mainly as an antibody. It is found in blood and extracellular fluid making it one of the most prevalent antibodies in the human body. IgG weighs approximately 150 kDa and it is expressed by B cells as part of the humoral response to pathogens. Alternate names include gamma globulin and Ig gamma. IgG antibodies form a critical aspect of adaptive immunity by targeting specific antigens for neutralization or destruction.
Biological function summary

IgG participates in several immune processes by binding to antigens and forming immune complexes. It facilitates pathogen opsonization initiating phagocytosis by immune cells like macrophages and neutrophils. IgG also activates the classical complement pathway which enhances pathogen clearance. IgG exists in a monomeric form and can cross the placenta providing passive immunity to the fetus. In laboratory settings its high specificity makes it valuable in techniques like IgG ELISA for detecting antigen presence.

Pathways

IgG plays an integral role in the complement system and the adaptive immune response. It partners with proteins such as C1q to initiate the classical complement pathway amplifying the body's ability to detect and remove pathogens. IgG also interacts with Fc receptors on immune cells modulating immune cell activity and facilitating antigen presentation to T cells. These interactions enhance the immune system's efficiency in recognizing and responding to diverse pathogens.

IgG has connections to autoimmune diseases and chronic infections. Elevated levels of IgG can indicate autoimmune conditions like lupus or rheumatoid arthritis where the immune system mistakenly attacks healthy tissue. During chronic infections such as HIV variations in IgG responses can affect disease progression and treatment efficacy. In these contexts IgG interacts with proteins involved in the immune dysregulation characteristic of such diseases highlighting its significant role in both protection and pathology.
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Product protocols

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Target data

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268). Mediates IgG effector functions on monocytes triggering ADCC of virus-infected cells.
See full target information IGHG1
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Publications (37)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in endocrinology 15:1523931 PubMed39850478

2025

Efficacy of cartilage-targeted IGF-1 in a mouse model of growth hormone insensitivity.

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Krishma Tailor,Janine van Ree,Timothy Stowe,Brit Ventura,Connor Sisk,Joanna Courtis,Anna Camp,Fatima Elzamzami,Jan van Deursen,Robert O'Brien,Jeffrey Baron,Julian C Lui

Science advances 11:eadt5464 PubMed39813350

2025

Rapid and comprehensive detection of viral antibodies and nucleic acids via an acoustofluidic integrated molecular diagnostics chip: AIMDx.

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Jiao Qian,Jianping Xia,Samantha Chiang,Jessica F Liu,Ke Li,Feng Li,Fang Wei,Mohammad Aziz,Yong Kim,Vinson Go,James Morizio,Ruoyu Zhong,Ye He,Kaichun Yang,Otto O Yang,David T W Wong,Luke P Lee,Tony Jun Huang

ACS biomaterials science & engineering 10:6451-6464 PubMed39269225

2024

Sclerostin Antibody-Loaded Dense Collagen Hydrogels Promote Critical-Size Bone Defect Repair.

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Ludovic Sicard,Sophie Maillard,Daline Mbita Akoa,Coralie Torrens,Anne-Margaux Collignon,Thibaud Coradin,Catherine Chaussain

American journal of translational research 16:4042-4051 PubMed39262721

2024

Antibiotic bone cement combined with vacuum sealing drainage effectively repairs sacrococcygeal pressure ulcer.

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Yanping Du,Yanan Yu,Shaona Xu,Jie Yang,Ying Liu,Yu Tang,Cuiying Chu

Journal of inflammation (London, England) 21:30 PubMed39135051

2024

Evidence of aberrant anti-epstein-barr virus antibody response, though no viral reactivation, in people with post-stroke fatigue.

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Isobel C Mouat,Li Zhu,Alperen Aslan,Barry W McColl,Stuart M Allan,Craig J Smith,Marion S Buckwalter,Laura McCulloch

Brazilian oral research 38:e043 PubMed38747830

2024

Salivary immunoglobulin levels and periodontal indices in Brazilian children with and without type 1 diabetes.

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Thyciana Rodrigues Ribeiro,Sara Maria Silva,Renata Asfor Rocha Carvalho Martins,Cláudia Ferreira Santos,Paulo Goberlânio de Barros Silva,Adriana Costa E Forti,Fábio Wildson Gurgel Costa,Manassés Claudino Fonteles,Cristiane Sá Roriz Fonteles

Frontiers in microbiology 15:1325558 PubMed38328418

2024

Recombinant Newcastle disease viruses expressing immunological checkpoint inhibitors induce a pro-inflammatory state and enhance tumor-specific immune responses in two murine models of cancer.

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Lisa A Santry,Jacob P van Vloten,Amanda W K AuYeung,Robert C Mould,Jacob G E Yates,Thomas M McAusland,James J Petrik,Pierre P Major,Byram W Bridle,Sarah K Wootton

The Journal of clinical investigation 134: PubMed38015619

2023

Disease-associated AIOLOS variants lead to immune deficiency/dysregulation by haploinsufficiency and redefine AIOLOS functional domains.

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Species

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Hye Sun Kuehn,Inga S Sakovich,Julie E Niemela,Agustin A Gil Silva,Jennifer L Stoddard,Ekaterina A Polyakova,Ana Esteve Sole,Svetlana N Aleshkevich,Tatjana A Uglova,Mikhail V Belevtsev,Vladislav R Vertelko,Tatsiana V Shman,Aleksandra N Kupchinskaya,Jolan E Walter,Thomas A Fleisher,Luigi D Notarangelo,Xiao P Peng,Ottavia M Delmonte,Svetlana O Sharapova,Sergio D Rosenzweig

Nature communications 14:6959 PubMed37907518

2023

Peripheral helper-T-cell-derived CXCL13 is a crucial pathogenic factor in idiopathic multicentric Castleman disease.

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Species

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Takuya Harada,Yoshikane Kikushige,Toshihiro Miyamoto,Kazuko Uno,Hiroaki Niiro,Atsushi Kawakami,Tomohiro Koga,Koichi Akashi,Kazuyuki Yoshizaki

Nature communications 14:6380 PubMed37821447

2023

SARS-CoV-2 infection of human lung epithelial cells induces TMPRSS-mediated acute fibrin deposition.

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Species

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Rachel Erickson,Chang Huang,Cameron Allen,Joanna Ireland,Gwynne Roth,Zhongcheng Zou,Jinghua Lu,Bernard A P Lafont,Nicole L Garza,Beniah Brumbaugh,Ming Zhao,Motoshi Suzuki,Lisa Olano,Joseph Brzostowski,Elizabeth R Fischer,Homer L Twigg,Reed F Johnson,Peter D Sun
View all publications
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