Name: Human IgG ELISA Kit
SKU: ab195215
Rating: 4 (6 reviews)

Human IgG ELISA kit is a single-wash, 90-minute SimpleStep ELISA® assay designed for the quantification of human Immunoglobulin G (IgG) with a sensitivity of 0.02 ng/mL. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.

- Colorimetric sandwich ELISA - 450nm readout; works on any plate reader
- High-throughput options – available in 96-well and 384-well formats, supporting large-scale sample processing.
- Broad sample compatibility – works with serum, plasma, cell culture supernatants, and other biological fluids
- Cited in over 20 publications

Images

Sandwich ELISA - Human IgG ELISA Kit (AB195215), expandable thumbnail

Publications

Key facts

Detection method: Colorimetric
Sample types: Tissue Lysate, Cerebral Spinal Fluid, Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma
Assay type: Sandwich (quantitative)
Reactive species: Human
Range: 0.23 - 15 ng/mL
Assay time: 1h 30m
Sensitivity: = 0.02 ng/mL

Reactivity data

Application	Reactivity	Dilution info	Notes
Application sELISA	Reactivity Reacts	Dilution info -	Notes -

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

See full target information

Function

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268). Mediates IgG effector functions on monocytes triggering ADCC of virus-infected cells.

Alternative names

Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1

What's included?

1 x 384 Tests

Components

10X Human IgG Capture Antibody

1 x 600 µL

10X Human IgG Detector Antibody

1 x 600 µL

10X Wash Buffer PT (ab206977)

2 x 20 mL

384 well CaptSure™ microplates

1 x 1 Unit

5X Cell Extraction Buffer PTR (ab193970)

1 x 50 mL

Antibody Diluent CP2

1 x 6 mL

Human IgG Lyophilized Purified Protein

2 x 1 Vial

Plate Seals

1 x 1 Unit

Sample Diluent NS (ab193972)

2 x 50 mL

Stop Solution

2 x 12 mL

TMB Development Solution

2 x 12 mL

Key facts

Detection method: Colorimetric
Sample types: Tissue Lysate, Cerebral Spinal Fluid, Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma
Assay type: Sandwich (quantitative)
Reactive species: Human
Range: 0.23 - 15 ng/mL
Assay time: 1h 30m
Assay Platform: Microplate
Sensitivity: = 0.02 ng/mL

Precision

Intra assay

Sample	n	mean	SD	C.V.
Sample Serum	n 8	mean -	SD -	C.V. 6.4

Inter assay

Sample	n	mean	SD	C.V.
Sample Serum	n 3	mean -	SD -	C.V. 14.7

Recovery

Sample specific recovery

Sample type	Average %	Range
Sample type Milk	Average % = 89	Range 83 - 94 %
Sample type Serum	Average % = 101	Range 88 - 125 %
Sample type EDTA Plasma	Average % = 90.17	Range 87 - 93 %
Sample type Cerebral Spinal Fluid	Average % = 98	Range 88 - 105 %
Sample type Urine	Average % = 87	Range 82 - 93 %
Sample type Saliva	Average % = 89	Range 80 - 106 %
Sample type Heparin Plasma	Average % = 100	Range 100 - 100 %
Sample type Citrate plasma	Average % = 100	Range 98 - 102 %
Sample type Cell culture media	Average % = 107	Range 96 - 115 %

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: +4°C

Notes

Human IgG ELISA Kit ab195215 is a rapid single-wash 90-min ELISA kit to measure Human IgG in tissue lysate, cerebral spinal fluid, saliva, urine, cell culture supernatant, milk, serum and plasma. This SimpleStep ELISA® sensitivity is 0.02 ng/mL.

How the assay works

Human IgG SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Human IgM, human IgA and human IgE were prepared at 10 ng/mL and 250 ng/mL in Sample Diluent NS and assayed for cross reactivity. No cross-reactivity was observed for IgM or IgE at either concentration with a mean OD deviation from background of -0.01. No cross-reactivity was observed for IgA at 10 ng/mL and only 0.4% cross-reactivity at 250 ng/mL of IgA. Purified human IgG was assayed at 5 ng/mL in the presence and absence of 250 ng/mL of human IgM, human IgA and human IgE to determine interference. After background subtraction, recovery of human IgG was observed at a mean of 98% with a standard deviation of 0.06.

Human IgG ELISA Kit ab195215 protocol summary:

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Human IgG ELISA Kit ab195215

Human IgG ELISA Kit ab195215 has been used to study human immunoglobulin g in autoimmune diseases and infections in various physio-pathological contexts such as obesity⁽¹⁾and SARS-CovV-2⁽²⁾.

References:

⁽¹⁾Leonid Kagan et al. 2023, PMID: 36949370,
⁽²⁾Peter D Sun et al. 2023, PMID: 37821447

Save your precious samples

Our 384-well Human IgG ELISA Kit only requires a maxmum of 12.5µL

Related and recommended products

Human IgG ELISA Kit ab195215 is commonly used to measure immune system functioning.

Other ELISA kits to measure Immunoglobulins include:

- Human IgM ELISA kit Human IgM ELISA Kit ab214568
- Human IgA ELISA kit Human IgA ELISA Kit ab196263
- Human IgD ELISA kit Human IgD ELISA Kit ab320794
- Human IgE ELISA kit Human IgE ELISA Kit ab195216

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

14 product images

Sandwich ELISA - Human IgG ELISA Kit (ab195215)
IgG levels in individual healthy donors.
Ten individual healthy donors were evaluated for the presence of IgG in serum using this assay. Results were interpolated from the standard curve in Sample Diluent NS and corrected for sample dilution (1:5x10⁶). The mean level of IgG was found at 12.5 mg/mL with a range of 9 – 18.4mg/mL.
Sandwich ELISA - Human IgG ELISA Kit (ab195215)
Example of Human IgG standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human IgG ELISA Kit (ab195215)
Raw data values for example of Human IgG standard curve in Sample Diluent NS.
Raw data values are shown in the table
Sandwich ELISA - Human IgG ELISA Kit (ab195215)
Example of Human IgG standard curve in 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human IgG ELISA Kit (ab195215)
Raw data values for example of Human IgG standard curve in 1X Cell Extraction Buffer PTR.
Raw data values are shown in the table
Sandwich ELISA - Human IgG ELISA Kit (ab195215)
Comparison of IgG levels in Human milk, urine and saliva.
Bodily fluids from 3 different donors were evaluated for the presence of IgG using this assay. Results were interpolated from the standard curve in sample diluent NS and corrected for sample dilution (1:2.5x10⁴). The mean levels in Milk were found at 20.7 μg/mL, in Urine at 0.8 μg/mL and in Saliva at 11.1 μg/mL.
ELISA - Human IgG ELISA Kit (ab195215)
IgG1-4 isotypes are detected by this kit.
Human IgG1, IgG2, IgG3 and IgG4 were tested at 5ng/mL
Sandwich ELISA - Human IgG ELISA Kit (ab195215)
Linearity of dilution – native IgG in human serum, plasma (citrate, EDTA and Heparin), and culture media.
Native IgG was measured in the human serum, plasma citrate, plasma EDTA, plasma heparin and culture media in a 2-fold dilution series. Sample dilutions were made in Sample Diluent NS for all samples, purified IgG was spiked into culture media and diluted in a 2-fold dilution series in Sample Diluent NS
Sandwich ELISA - Human IgG ELISA Kit (ab195215)
Linearity of dilution – native IgG in human milk, urine, saliva and HLH extract.
Native IgG was measured in milk, saliva, urine and liver homogenate (HLH) in a 2-fold dilution series. Sample dilutions were made in Sample Diluent NS for all samples except for HLH, which was carried out in 1X cell extraction buffer PTR.
Sandwich ELISA - Human IgG ELISA Kit (ab195215)
Interpolated concentration of native IgG was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1:1,000,000 human serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human IgG ELISA Kit (ab195215)
Interpolated concentration of native IgG was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 100 ng/mL human liver extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
Sandwich ELISA - Human IgG ELISA Kit (ab195215)
Example of Human IgG standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human IgG ELISA Kit (ab195215)
Example of Human IgG standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human IgG ELISA Kit (ab195215)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.