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AB317543

Human IgG1 ELISA Kit

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Human IgG1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IgG1 with a sensitivity of 6.237 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1

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Sandwich ELISA - Human IgG1 ELISA Kit (AB317543)
  • sELISA

Lab

Sandwich ELISA - Human IgG1 ELISA Kit (AB317543)

Cross reactivity of related immunoglobulin proteins. 50 ng/mL of natively purified human IgG2, IgG3, IgG4, IgA, IgD, IgE, and IgM were tested individually for cross-reactivity. Percent reactivity was determined by interpolating the protein concentration from the human IgG1 standard curve and expressing the interpolated concentration as a percentage of the protein concentration tested.

Sandwich ELISA - Human IgG1 ELISA Kit (AB317543)
  • sELISA

Supplier Data

Sandwich ELISA - Human IgG1 ELISA Kit (AB317543)

Example of human IgG1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IgG1 ELISA Kit (AB317543)
  • sELISA

Supplier Data

Sandwich ELISA - Human IgG1 ELISA Kit (AB317543)

Interpolated concentration of native IgG1 was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 1,000 urine and 1 : 1,000 saliva.  The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT.

Sandwich ELISA - Human IgG1 ELISA Kit (AB317543)
  • sELISA

Supplier Data

Sandwich ELISA - Human IgG1 ELISA Kit (AB317543)

Interpolated concentration of native IgG1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 1,000 saliva.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT.

Sandwich ELISA - Human IgG1 ELISA Kit (AB317543)
  • sELISA

Supplier Data

Sandwich ELISA - Human IgG1 ELISA Kit (AB317543)

Example of human IgG1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IgG1 ELISA Kit (AB317543)
  • sELISA

Supplier Data

Sandwich ELISA - Human IgG1 ELISA Kit (AB317543)

Interpolated concentration of native IgG1 was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 8,000,000 serum, plasma (EDTA), plasma (citrate), and plasma (heparin).  The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT.

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Urine, Saliva, Cell culture media

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 6.237 pg/mL

Range

21.875 - 1400 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human IgG1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IgG1 protein in Serum, EDTA Plasma, Cit Plasma, Hep Plasma, Urine, Saliva, Cell culture media. Quantitate Human IgG1 with 6.237 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)

Also available in a fully validated 384-well format.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.6" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.3" } ]
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Recovery

[ { "sample": "Serum", "range": "95 - 104 %", "average": "= 100" }, { "sample": "EDTA Plasma", "range": "69 - 95 %", "average": "= 82" }, { "sample": "Citrate plasma", "range": "91 - 99 %", "average": "= 94" }, { "sample": "Heparin Plasma", "range": "81 - 94 %", "average": "= 88" }, { "sample": "Urine", "range": "92 - 108 %", "average": "= 100" }, { "sample": "Saliva", "range": "92 - 95 %", "average": "= 94" }, { "sample": "Cell culture media", "range": "91 - 97 %", "average": "= 94" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Constant region of immunoglobulin (Ig) heavy chains. Igs are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound Igs serve as receptors, which upon binding to a specific antigen trigger the clonal expansion and differentiation of B lymphocytes into Ig-secreting plasma cells. Secreted Igs known as antibodies mediate the effector phase of humoral immunity by blocking the interaction of infectious antigens with cellular receptors (via the antigen-binding region) and eliciting effector mechanisms that lead to pathogen neutralization (via the constant region) (PubMed : 17576170, PubMed : 20176268, PubMed : 22158414). The antigen-binding region is formed by the variable domain of one heavy chain paired with the variable domain of its associated light chain. Each Ig molecule has two antigen-binding sites with remarkable affinity for a particular antigen due to V-(D)-J rearrangement, somatic hypermutations and affinity maturation of the variable domains upon antigen exposure (PubMed : 17576170, PubMed : 20176268, PubMed : 22158414). The constant region defines the Ig isotype that perform distinct sets of effector functions. B cells diversify and rearrange their Ig constant regions through class-switch recombination, a process by which the constant region is switched from one Ig isotype to another, namely from IgM and IgD to IgG, IgA and IgE (PubMed : 17576170, PubMed : 20176268, PubMed : 22158414). The constant region of Ig gamma-1 (IgG1) isotype interacts (via the fragment crystallizable, Fc) with receptors on innate immune cells and the complement system to mediate humoral effector functions, including antibody-dependent cellular cytotoxicity or phagocytosis, complement-dependent cytotoxicity and inflammatory responses.
See full target information IGHG1
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Product promise

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