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AB326310

Human IgG2 ELISA Kit, Chemiluminescent

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Human IgG2 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human IgG2 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 0.82 - 600 ng/ml with a sensitivity of 0.28ng/mL.

-Patent pending

View Alternative Names

Immunoglobulin heavy constant gamma 2, Ig gamma-2 chain C region, Ig gamma-2 chain C region DOT, Ig gamma-2 chain C region TIL, Ig gamma-2 chain C region ZIE, IGHG2

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Sandwich ELISA - Human IgG2 ELISA Kit, Chemiluminescent (AB326310)
  • sELISA

Lab

Sandwich ELISA - Human IgG2 ELISA Kit, Chemiluminescent (AB326310)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human IgG2 ELISA Kit, Chemiluminescent (AB326310)
  • sELISA

Lab

Sandwich ELISA - Human IgG2 ELISA Kit, Chemiluminescent (AB326310)

Dynamic range comparison between the Chemiluminescent Human IgG2 SimpleStep ELISA, colorimetric Human IgG2 SimpleStep ELISA (ab202402), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows comparable sensitivity and increased range.

Sandwich ELISA - Human IgG2 ELISA Kit, Chemiluminescent (AB326310)
  • sELISA

Lab

Sandwich ELISA - Human IgG2 ELISA Kit, Chemiluminescent (AB326310)

Standard curve comparison between the Chemiluminescent Human IgG2 SimpleStep ELISA and colorimetric Human IgG2 SimpleStep ELISA (ab202402) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IgG2 ELISA Kit, Chemiluminescent (AB326310)
  • sELISA

Supplier Data

Sandwich ELISA - Human IgG2 ELISA Kit, Chemiluminescent (AB326310)

Linearity of dilution of native IgG2 in human citrate EDTA and heparin plasmas.

Native human IgG2 protein was measured in citrate plasma (1 : 300000) EDTA plasma (1 : 200000) and heparin plasma (1 : 300000) in a 2 fold dilution series in Sample Diluent NS. The interpolated dilution factor corrected values are graphed (mean +/- SD).

Sandwich ELISA - Human IgG2 ELISA Kit, Chemiluminescent (AB326310)
  • sELISA

Supplier Data

Sandwich ELISA - Human IgG2 ELISA Kit, Chemiluminescent (AB326310)

Levels of IgG2 in human donors.

Ten individual healthy male donors were evaluated for the presence of IgG2 in serum using this assay. Results were interpolated from the standard curve in Sample Diluent NS and corrected by sample dilution (1 : 500000). In the 10 individual donors the mean level of IgG2 is 5.6 mg/mL with a range of 2.5 – 9.1 mg/mL and a standard deviation of 2.16 mg/mL.

Sandwich ELISA - Human IgG2 ELISA Kit, Chemiluminescent (AB326310)
  • sELISA

Supplier Data

Sandwich ELISA - Human IgG2 ELISA Kit, Chemiluminescent (AB326310)

Linearity of dilution of native IgG2 in human milk urine and saliva.

Native human IgG2 protein was measured in milk (1 : 800) urine (1 : 30) and saliva (1 : 400) in a 2-fold dilution series in Sample Diluent NS. The interpolated dilution factor corrected values are graphed (mean +/- SD).

Key facts

Detection method

Luminescent

Sample types

Milk, Serum, EDTA Plasma, Urine, Saliva, Heparin Plasma, Citrate plasma, Cell culture media

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.28 ng/mL

Range

0.82 - 600 ng/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human IgG2 ELISA Kit, Chemiluminescent (ab326310) is a rapid single-wash sandwich 55-min ELISA to measure Human IgG2 in Milk, Serum, EDTA Plasma, Urine, Saliva, Heparin Plasma, Citrate plasma, Cell culture media with a sensitivity of 0.28 ng/mL.

How the assay works

Human IgG2 ELISA Kit, Chemiluminescent (ab326310) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human IgG2 (ab326310) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection
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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.3" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.3" } ]
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Recovery

[ { "sample": "Milk", "range": "86 - 94 %", "average": "= 90" }, { "sample": "Serum", "range": "97.4 - 102.4 %", "average": "= 99.7" }, { "sample": "EDTA Plasma", "range": "98 - 105 %", "average": "= 102.5" }, { "sample": "Urine", "range": "90 - 101 %", "average": "= 96.5" }, { "sample": "Saliva", "range": "93 - 124 %", "average": "= 104" }, { "sample": "Heparin Plasma", "range": "97.4 - 107.4 %", "average": "= 101.3" }, { "sample": "Citrate plasma", "range": "103 - 110 %", "average": "= 107.5" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab326310-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Human IgG2 Capture Antibody (Lyophilized)", "number":"AB326310-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB326310-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB326310-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IgG2 Detector Antibody", "number":"AB326310-CMP02", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB326310-CMP08", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB326310-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB326310-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IgG2 Lyophilized Purified Protein", "number":"AB326310-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB326310-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB326310-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Constant region of immunoglobulin (Ig) heavy chains. Igs are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound Igs serve as receptors, which upon binding to a specific antigen trigger the clonal expansion and differentiation of B lymphocytes into Ig-secreting plasma cells. Secreted Igs known as antibodies mediate the effector phase of humoral immunity by blocking the interaction of infectious antigens with cellular receptors (via the antigen-binding region) and eliciting effector mechanisms that lead to pathogen neutralization (via the constant region) (PubMed : 17576170, PubMed : 20176268, PubMed : 22158414). The antigen-binding region is formed by the variable domain of one heavy chain paired with the variable domain of its associated light chain. Each Ig molecule has two antigen-binding sites with remarkable affinity for a particular antigen due to V-(D)-J rearrangement, somatic hypermutations and affinity maturation of the variable domains upon antigen exposure (PubMed : 17576170, PubMed : 20176268, PubMed : 22158414). The constant region defines the Ig isotype that perform distinct sets of effector functions. B cells diversify and rearrange their Ig constant regions through class-switch recombination, a process by which the constant region is switched from one Ig isotype to another, namely from IgM and IgD to IgG, IgA and IgE (PubMed : 17576170, PubMed : 20176268, PubMed : 22158414). The constant region interacts (via the fragment crystallizable, Fc) with receptors on innate immune cells and the complement system to mediate humoral effector functions. Ig gamma-2 (IgG2) isotype formed against polysaccharide structures found on encapsulated bacteria elicits antibody-dependent protection through activation of complement pathway.
See full target information IGHG2
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websiteProtocolBooklet
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Product promise

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