Human IgG3 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human IgG3 in Human in Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine samples.
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- International journal of molecular sciences 22:2021Salivary Immunoglobulin Gamma-3 Chain C Is a Promising Noninvasive Biomarker for Systemic Lupus Erythematosus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJu-Yang Jung,Jin-Young Nam,Keun-Sil Ryu,In-Ok Son,Joo-Ho Shin,Wook-Young Baek,Hyoun-Ah Kim,Chang-Hee SuhPubMed 33573068
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.391 - 25 ng/mL
- Assay time
- 4h
- Sensitivity
- > 0.14 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268).
Alternative names
Immunoglobulin heavy constant gamma 3, HDC, Heavy chain disease protein, Ig gamma-3 chain C region, IGHG3
What's included?
Recommended products
Human IgG3 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human IgG3 in Human in Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.391 - 25 ng/mL
- Assay time
- 4h
- Assay Platform
- Microplate
- Sensitivity
- > 0.14 ng/mL
- General recovery
- 102%
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 0 | C.V. 6.5 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 0 | C.V. 9.5 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
Abcam's IgG3 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IgG3 in plasma, serum, milk, urine, saliva, CSF, cell culture, cell lysate, and tissue samples.
An IgG3 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an IgG3 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IgG3 captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Product promise
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1 product image
Sandwich ELISA - Human IgG3 ELISA Kit (ab137981)
Representative Standard Curve using ab137981
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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