AB318929

Human IgG3 ELISA Kit

Recombinant
SimpleStep
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Human IgG3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IgG3 with a sensitivity of 59.929 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Immunoglobulin heavy constant gamma 3, HDC, Heavy chain disease protein, Ig gamma-3 chain C region, IGHG3

7 Images

Sandwich ELISA - Human IgG3 ELISA Kit (AB318929)

sELISA

Supplier Data

Sandwich ELISA - Human IgG3 ELISA Kit (AB318929)

Cross reactivity of related immunoglobulin proteins. 50 ng/mL of natively purified human IgG1 IgG2, IgG4, IgA, IgD, IgE, and IgM were tested individually for cross-reactivity. Percent reactivity was determined by interpolating the protein concentration from the human IgG3 standard curve and expressing the interpolated concentration as a percentage of the protein concentration tested.

sELISA

Supplier Data

Sandwich ELISA - Human IgG3 ELISA Kit (AB318929)

Interpolated concentrations of IgG3 in normal human serum donors. Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IgG3 concentration was determined to be 340 μg/mL with a range of 163 – 850 μg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human IgG3 ELISA Kit (AB318929)

Interpolated concentrations of human IgG3 in serum, plasma (citrate), plasma (EDTA), and plasma (heparin). Interpolated concentration of native IgG3 was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 50,000 serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT.

sELISA

Supplier Data

Sandwich ELISA - Human IgG3 ELISA Kit (AB318929)

Interpolated concentrations of human IgG3 in urine in 96-well vs. 384-well plates. Interpolated concentration of native IgG3 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 10% urine. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT.

sELISA

Supplier Data

Sandwich ELISA - Human IgG3 ELISA Kit (AB318929)

Interpolated concentrations of human IgG3 in urine, saliva, and milk. Interpolated concentration of native IgG3 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : urine 10%, saliva 2.5%, and milk 4%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT.

sELISA

Supplier Data

Sandwich ELISA - Human IgG3 ELISA Kit (AB318929)

Example of human IgG3 standard curve in 1X Wash Buffer PT. Example of human IgG3 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human IgG3 ELISA Kit (AB318929)

Example of human IgG3 standard curve in 1X Wash Buffer PT in 96-well vs. 384-well plate. Example of human IgG3 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma, Cell culture media, Citrate plasma, Heparin Plasma, Urine, Milk, Saliva

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 59.929 pg/mL

Range

195.313 - 12500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

style

left-column

Product details

Human IgG3 ELISA kit (ab318929) is a rapid single-wash sandwich 90-min ELISA to measure Human IgG3 in Serum, EDTA Plasma, Cell culture media, Citrate plasma, Heparin Plasma, Urine, Milk, Saliva. This SimpleStep sensitivity is 59.929 pg/mL.

How the assay works

Human IgG3 ELISA kit (ab318929) SimpleStep ELISA^® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human IgG3 (ab318929) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our Human IgG3 ELISA kit only requires 12.5µL sample.

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-IgG3 antibody [EPR26763-67] - BSA and Azide free (Capture) ab314155
- Anti-IgG3 antibody [EPR26763-83] - BSA and Azide free (Detector) ab314156

style

left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.3" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.3" } ]

style

left-column

Recovery

[ { "sample": "Serum", "range": "96 - 108 %", "average": "= 100" }, { "sample": "Citrate plasma", "range": "103 - 115 %", "average": "= 108" }, { "sample": "EDTA Plasma", "range": "101 - 113 %", "average": "= 106" }, { "sample": "Heparin Plasma", "range": "98 - 118 %", "average": "= 107" }, { "sample": "Cell culture media", "range": "76 - 95 %", "average": "= 85" }, { "sample": "Urine", "range": "94 - 98 %", "average": "= 96" }, { "sample": "Saliva", "range": "97 - 112 %", "average": "= 103" }, { "sample": "Milk", "range": "95 - 125 %", "average": "= 109" } ]

style

left-column

What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab318929-10x96Tests", "assetComponentDetails": [ { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB318929-CMP10" }, { "size":"10 x 96 Wells", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB318929-CMP09" }, { "size":"10 x 600 µL", "name":"10X Human IgG3 Detector Antibody", "number":"AB318929-CMP02" }, { "size":"10 x 2 Vial", "name":"Human IgG3 Lyophilized Recombinant Protein", "number":"AB318929-CMP03" }, { "size":"2 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB318929-CMP05" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB318929-CMP08" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB318929-CMP07" }, { "size":"10 x 1 Vial", "name":"Lyophilized Human IgG3 Capture Antibody", "number":"AB318929-CMP01" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB318929-CMP06" }, { "size":"10 x 6 mL", "name":"Antibody Diluent CP2", "number":"AB318929-CMP04" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab318929-1x384Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB318929-CMP10" }, { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB318929-CMP08" }, { "size":"1 x 1 Vial", "name":"Lyophilized Human IgG3 Capture Antibody", "number":"AB318929-CMP01" }, { "size":"1 x 384 Wells", "name":"384 well CaptSure™ microplates", "number":"AB318929-CMP11" }, { "size":"2 x 1 Vial", "name":"Human IgG3 Lyophilized Recombinant Protein", "number":"AB318929-CMP03" }, { "size":"1 x 600 µL", "name":"10X Human IgG3 Detector Antibody", "number":"AB318929-CMP02" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB318929-CMP06" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CP2", "number":"AB318929-CMP04" }, { "size":"2 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB318929-CMP05" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB318929-CMP07" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab318929-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB318929-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CP2", "number":"AB318929-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB318929-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB318929-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB318929-CMP07", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Lyophilized Human IgG3 Capture Antibody", "number":"AB318929-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IgG3 Lyophilized Recombinant Protein", "number":"AB318929-CMP03", "productcode":"" }, { "size":"2 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB318929-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IgG3 Detector Antibody", "number":"AB318929-CMP02", "productcode":"" }, { "size":"1 x 96 Wells", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB318929-CMP09", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

style

left-column

Product protocols

style

left-column

Target data

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268).

See full target information IGHG3

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human IgG3 ELISA Kit

Sandwich ELISA - Human IgG3 ELISA Kit (AB318929)

Sandwich ELISA - Human IgG3 ELISA Kit (AB318929)

Sandwich ELISA - Human IgG3 ELISA Kit (AB318929)

Sandwich ELISA - Human IgG3 ELISA Kit (AB318929)

Sandwich ELISA - Human IgG3 ELISA Kit (AB318929)

Sandwich ELISA - Human IgG3 ELISA Kit (AB318929)

Sandwich ELISA - Human IgG3 ELISA Kit (AB318929)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Assay Kits

Secondary Antibodies

Primary Antibodies

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Product promise