AB318929

Human IgG3 ELISA Kit

Recombinant
SimpleStep
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Human IgG3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IgG3 with a sensitivity of 59.929 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Immunoglobulin heavy constant gamma 3, HDC, Heavy chain disease protein, Ig gamma-3 chain C region, IGHG3

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Sandwich ELISA - Human IgG3 ELISA Kit (AB318929)

sELISA

Supplier Data

Sandwich ELISA - Human IgG3 ELISA Kit (AB318929)

Cross reactivity of related immunoglobulin proteins. 50 ng/mL of natively purified human IgG1 IgG2, IgG4, IgA, IgD, IgE, and IgM were tested individually for cross-reactivity. Percent reactivity was determined by interpolating the protein concentration from the human IgG3 standard curve and expressing the interpolated concentration as a percentage of the protein concentration tested.

sELISA

Supplier Data

Sandwich ELISA - Human IgG3 ELISA Kit (AB318929)

Interpolated concentrations of IgG3 in normal human serum donors. Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IgG3 concentration was determined to be 340 μg/mL with a range of 163 – 850 μg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human IgG3 ELISA Kit (AB318929)

Interpolated concentrations of human IgG3 in serum, plasma (citrate), plasma (EDTA), and plasma (heparin). Interpolated concentration of native IgG3 was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 50,000 serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT.

sELISA

Supplier Data

Sandwich ELISA - Human IgG3 ELISA Kit (AB318929)

Interpolated concentrations of human IgG3 in urine in 96-well vs. 384-well plates. Interpolated concentration of native IgG3 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 10% urine. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT.

sELISA

Supplier Data

Sandwich ELISA - Human IgG3 ELISA Kit (AB318929)

Interpolated concentrations of human IgG3 in urine, saliva, and milk. Interpolated concentration of native IgG3 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : urine 10%, saliva 2.5%, and milk 4%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT.

sELISA

Supplier Data

Sandwich ELISA - Human IgG3 ELISA Kit (AB318929)

Example of human IgG3 standard curve in 1X Wash Buffer PT. Example of human IgG3 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human IgG3 ELISA Kit (AB318929)

Example of human IgG3 standard curve in 1X Wash Buffer PT in 96-well vs. 384-well plate. Example of human IgG3 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma, Cell culture media, Citrate plasma, Heparin Plasma, Urine, Milk, Saliva

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 59.929 pg/mL

Range

195.313 - 12500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Product details

Human AMH SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of AMH protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture media. Quantitate Human AMH with 18.389 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.3" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.3" } ]

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Recovery

[ { "sample": "Serum", "range": "96 - 108 %", "average": "= 100" }, { "sample": "Citrate plasma", "range": "103 - 115 %", "average": "= 108" }, { "sample": "EDTA Plasma", "range": "101 - 113 %", "average": "= 106" }, { "sample": "Heparin Plasma", "range": "98 - 118 %", "average": "= 107" }, { "sample": "Cell culture media", "range": "76 - 95 %", "average": "= 85" }, { "sample": "Urine", "range": "94 - 98 %", "average": "= 96" }, { "sample": "Saliva", "range": "97 - 112 %", "average": "= 103" }, { "sample": "Milk", "range": "95 - 125 %", "average": "= 109" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268).

See full target information IGHG3

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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