JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB325734

Human IgG3 ELISA Kit, Chemiluminescent

Be the first to review this product! Submit a review

|

(0 Publication)

Human IgG3 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human IgG3 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 34.29 - 75000 pg/ml with a sensitivity of 22.68 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

View Alternative Names

Immunoglobulin heavy constant gamma 3, HDC, Heavy chain disease protein, Ig gamma-3 chain C region, IGHG3

7 Images
Sandwich ELISA - Human IgG3 ELISA Kit, Chemiluminescent (AB325734)
  • sELISA

Lab

Sandwich ELISA - Human IgG3 ELISA Kit, Chemiluminescent (AB325734)

Interpolated concentrations of human IgG3 in urine, saliva, and milk. Interpolated concentration of native IgG3 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : urine 10%, saliva 2.5%, and milk 4%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT.

Sandwich ELISA - Human IgG3 ELISA Kit, Chemiluminescent (AB325734)
  • sELISA

Lab

Sandwich ELISA - Human IgG3 ELISA Kit, Chemiluminescent (AB325734)

Cross reactivity of related immunoglobulin proteins. 50 ng/mL of natively purified human IgG1 IgG2, IgG4, IgA, IgD, IgE, and IgM were tested individually for cross-reactivity. Percent reactivity was determined by interpolating the protein concentration from the human IgG3 standard curve and expressing the interpolated concentration as a percentage of the protein concentration tested.

Sandwich ELISA - Human IgG3 ELISA Kit, Chemiluminescent (AB325734)
  • sELISA

Lab

Sandwich ELISA - Human IgG3 ELISA Kit, Chemiluminescent (AB325734)

Interpolated concentrations of IgG3 in normal human serum donors. Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IgG3 concentration was determined to be 340 µg/mL with a range of 163 – 850 µg/mL.

Sandwich ELISA - Human IgG3 ELISA Kit, Chemiluminescent (AB325734)
  • sELISA

Lab

Sandwich ELISA - Human IgG3 ELISA Kit, Chemiluminescent (AB325734)

Interpolated concentrations of human IgG3 in serum, plasma (citrate), plasma (EDTA), and plasma (heparin). Interpolated concentration of native IgG3 was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 50,000 serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT.

Sandwich ELISA - Human IgG3 ELISA Kit, Chemiluminescent (AB325734)
  • sELISA

Lab

Sandwich ELISA - Human IgG3 ELISA Kit, Chemiluminescent (AB325734)

Dynamic range comparison between the Chemiluminescent Human IgG3 SimpleStep ELISA, colorimetric Human IgG3 SimpleStep ELISA (ab318929), and leading chemiluminescent competitor. The Chemiluminescent SimpleStep ELISA kit shows comparable sensitivity and increased dynamic range.

Sandwich ELISA - Human IgG3 ELISA Kit, Chemiluminescent (AB325734)
  • sELISA

Lab

Sandwich ELISA - Human IgG3 ELISA Kit, Chemiluminescent (AB325734)

Standard curve comparison between the Chemiluminescent Human IgG3 SimpleStep ELISA and colorimetric Human IgG3 SimpleStep ELISA (ab318929) in 1X Wash Buffer PT. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IgG3 ELISA Kit, Chemiluminescent (AB325734)
  • sELISA

Lab

Sandwich ELISA - Human IgG3 ELISA Kit, Chemiluminescent (AB325734)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media, Urine, Saliva, Milk

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 22.68 pg/mL

Range

34.29 - 75000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human IgG3 ELISA Kit, Chemiluminescent (ab325734) is a rapid single-wash sandwich 55-min ELISA to measure Human IgG3 in Serum, Plasma – Citrate, Plasma – EDTA, Plasma – Heparin, Cell Culture Media, Urine, Saliva, Milk with a sensitivity of 22.68 pg/mL.

How the assay works

Human IgG3 ELISA Kit, Chemiluminescent (ab325734) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human IgG3 (ab325734) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-IgG3 antibody [EPR26763-67] - BSA and Aside free (Capture) ab314155
- Anti-IgG3 antibody [EPR26763-83] - BSA and Aside free (Detector) ab314156
style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.3" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.3" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "96 - 108 %", "average": "= 100" }, { "sample": "Citrate plasma", "range": "103 - 115 %", "average": "= 108" }, { "sample": "EDTA Plasma", "range": "101 - 113 %", "average": "= 106" }, { "sample": "Heparin Plasma", "range": "98 - 118 %", "average": "= 107" }, { "sample": "Urine", "range": "94 - 98 %", "average": "= 96" }, { "sample": "Saliva", "range": "97 - 112 %", "average": "= 103" }, { "sample": "Milk", "range": "95 - 125 %", "average": "= 109" }, { "sample": "Cell culture media", "range": "76 - 95 %", "average": "= 85" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab325734-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Human IgG3 Lyophilized Recombinant Protein", "number":"AB325734-CMP03", "productcode":"" }, { "size":"2 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB325734-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB325734-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Human IgG3 Capture Antibody", "number":"AB325734-CMP01", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CP2", "number":"AB325734-CMP04", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB325734-CMP07", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB325734-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IgG3 Detector Antibody", "number":"AB325734-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB325734-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB325734-CMP09", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268).
See full target information IGHG3
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com