Human IgG4 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human IgG4 in Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media, Urine, Saliva, Milk samples.
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Key facts
- Detection method
Colorimetric
- Sample types
Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media, Urine, Saliva, Milk
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
140.625 - 9000 pg/mL
- Assay time
1h 30m
- Sensitivity
= 40.048 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:22158414, PubMed:20176268). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268).
Alternative names
Immunoglobulin heavy constant gamma 4, Ig gamma-4 chain C region, IGHG4
What's included?
Recommended products
Human IgG4 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human IgG4 in Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media, Urine, Saliva, Milk samples.
Alternative names
Immunoglobulin heavy constant gamma 4, Ig gamma-4 chain C region, IGHG4
Key facts
- Detection method
Colorimetric
- Sample types
Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media, Urine, Saliva, Milk
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
140.625 - 9000 pg/mL
- Assay time
1h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 40.048 pg/mL
- General recovery
86%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 5.4 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 9.4 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 86 | Range 81 - 91 % |
Sample type Citrate plasma | Average % = 108 | Range 103 - 114 % |
Sample type EDTA Plasma | Average % = 102 | Range 98 - 106 % |
Sample type Heparin Plasma | Average % = 114 | Range 114 - 115 % |
Sample type Urine | Average % = 94 | Range 87 - 105 % |
Sample type Saliva | Average % = 108 | Range 99 - 121 % |
Sample type Milk | Average % = 95 | Range 84 - 104 % |
Sample type Cell culture media | Average % = 90 | Range 89 - 90 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Human IgG4 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IgG4 protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture media, Urine, Saliva, Milk. Quantitate Human IgG4 with 40.048 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IgG4 also known as IgG4-Fc or IGHG4 is one of the subclasses of Immunoglobulin G (IgG) antibodies. Mechanically it plays a role in the immune response by recognizing and binding to specific antigens. Its molecular weight is approximately 146 kDa which is typical for IgG molecules. IgG4 is commonly expressed in the blood and extracellular fluids. It can also be found at sites of chronic inflammation reflecting its involvement in modulating immune responses.
- Biological function summary
IgG4 antibodies act to regulate immune responses and prevent excessive inflammation. It does not engage in strong effector functions like other IgG subclasses due to its unique ability to exchange half-molecules leading to functional monovalency. This characteristic makes it part of an immune complex that can block other antibody functions by competitive binding without activating complement pathways. The IgG4 sequence shows unique properties that allow it to engage selectively in regulatory pathways which is a distinct attribute among immunoglobulins.
- Pathways
IgG4 functions within immune regulatory pathways that inhibit inflammation. It involves pathways like the Fc gamma receptor-mediated suppression of effector cell responses. Through this pathway IgG4 can interact with proteins such as Fc gamma receptors on cells altering signaling cascades that prevent unnecessary immune activation. IgG4 antibodies play these roles alongside other immune-modulating proteins helping to balance immune system activity.
- Associated diseases and disorders
IgG4 relates to conditions like IgG4-related disease (IgG4-RD) and autoimmune diseases where it presents an abnormal increase in serum levels and tissue infiltration. IgG4-RD is characterized by elevated IgG4 protein and causes fibrotic tissue inflammation in organs such as the pancreas and salivary glands. IgG4's interaction with proteins like T regulatory cells may contribute to downregulating immune attacks in autoimmune disease highlighting its dual role in both defensive and regulatory immune activities.
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7 product images
Sandwich ELISA - Human IgG4 ELISA Kit (ab320788)
Example of human IgG4 standard curve in 1X Wash Buffer PT. Example of human IgG4 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human IgG4 ELISA Kit (ab320788)
Interpolated concentrations of human IgG4 in serum, plasma (citrate), plasma (EDTA), and plasma (heparin). Interpolated concentration of native IgG4 was measured in duplicate at different sample concentrations. Undiluted samples are 1:100,000 serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT.
Sandwich ELISA - Human IgG4 ELISA Kit (ab320788)
Interpolated concentrations of human IgG4 in urine, saliva, and milk. Interpolated concentration of native IgG4 was measured in duplicate at different sample concentrations. Undiluted samples are as follows: urine 1:20, saliva 1:100, and milk 1:100. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT.
Sandwich ELISA - Human IgG4 ELISA Kit (ab320788)
Interpolated concentrations of IgG4 in normal human serum donors. Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean IgG4 concentration was determined to be 248 ?g/mL with a range of 102 – 566 ?g/mL.
Sandwich ELISA - Human IgG4 ELISA Kit (ab320788)
Example of human IgG4 standard curve in 1X Wash Buffer PT in 96-well vs. 384-well plate. Example of human IgG4 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human IgG4 ELISA Kit (ab320788)
Interpolated concentrations of human IgG4 in serum in 96-well vs. 384-well plates. Interpolated concentration of native IgG4 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1:100,000 serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT.
Sandwich ELISA - Human IgG4 ELISA Kit (ab320788)
Cross reactivity of related immunoglobulin proteins. 50 ng/mL of natively purified human IgG1, IgG2, IgG3, IgA, IgD, IgE, and IgM were tested individually for cross-reactivity. Percent reactivity was determined by interpolating the protein concentration from the human IgG4 standard curve and expressing the interpolated concentration as a percentage of the protein concentration tested.
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