Human IgG4 ELISA Kit, colorimetric, 90-min ELISA (ab320788)

Human IgG4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IgG4 with a sensitivity of 40.048 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

Be the first to review this product! Submit a review

Images

Sandwich ELISA - Human IgG4 ELISA Kit (AB320788), expandable thumbnail

Key facts

Detection method: Colorimetric
Sample types: Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media, Urine, Saliva, Milk
Assay type: Sandwich (quantitative)
Reactive species: Human
Range: 140.625 - 9000 pg/mL
Assay time: 1h 30m
Sensitivity: = 40.048 pg/mL

Reactivity data

Application	Reactivity	Dilution info	Notes
Application sELISA	Reactivity Reacts	Dilution info -	Notes -

Target data

See full target information

Function

The protein expressed by the IGHG4 gene is part of the constant region of immunoglobulin heavy chains. Immunoglobulins, or antibodies, are glycoproteins produced by B lymphocytes that can be membrane-bound or secreted. During humoral immunity's recognition phase, membrane-bound immunoglobulins act as receptors. When they bind a specific antigen, they trigger B lymphocytes to undergo clonal expansion and differentiate into plasma cells that secrete immunoglobulins. These secreted immunoglobulins then mediate the effector phase of humoral immunity to eliminate the bound antigens. Each immunoglobulin possesses two antigen binding sites, formed by the variable domains of a heavy chain and an associated light chain, allowing for high affinity binding to specific antigens. These variable domains undergo V-(D)-J rearrangement and may experience somatic hypermutations, facilitating affinity maturation after antigen exposure and selection. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

Immunoglobulin heavy constant gamma 4, Ig gamma-4 chain C region, IGHG4

What's included?

10 x 96 Tests

Components

10X Human IgG4 Capture Antibody

10 x 600 µL

10X Human IgG4 Detector Antibody

10 x 600 µL

10X Wash Buffer PT (ab206977)

2 x 200 mL

Antibody Diluent CP2

10 x 6 mL

Human IgG4 Lyophilized Recombinant Protein

20 x 1 Vial

Plate Seal

10 x 1 Unit

SimpleStep Pre-Coated 96-Well Microplate (ab206978)

10 x 1 Unit

Stop Solution

1 x 120 mL

TMB Development Solution

1 x 120 mL

Key facts

Detection method: Colorimetric
Sample types: Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture media, Urine, Saliva, Milk
Assay type: Sandwich (quantitative)
Reactive species: Human
Range: 140.625 - 9000 pg/mL
Assay time: 1h 30m
Assay Platform: Pre-coated microplate (12 x 8 well strips)
Sensitivity: = 40.048 pg/mL
General recovery: 86%

Precision

Intra assay

Sample	n	C.V.
Sample Serum	n 8	C.V. 5.4

Inter assay

Sample	n	C.V.
Sample Serum	n 3	C.V. 9.4

Recovery

Sample specific recovery

Sample type	Average %	Range
Sample type Serum	Average % = 86	Range 81 - 91 %
Sample type Citrate plasma	Average % = 108	Range 103 - 114 %
Sample type EDTA Plasma	Average % = 102	Range 98 - 106 %
Sample type Heparin Plasma	Average % = 114	Range 114 - 115 %
Sample type Urine	Average % = 94	Range 87 - 105 %
Sample type Saliva	Average % = 108	Range 99 - 121 %
Sample type Milk	Average % = 95	Range 84 - 104 %
Sample type Cell culture media	Average % = 90	Range 89 - 90 %

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: +4°C

Notes

Human IgG4 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IgG4 protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture media, Urine, Saliva, Milk. Quantitate Human IgG4 with 40.048 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

IgG4 also known as IgG4-Fc or IGHG4 is one of the subclasses of Immunoglobulin G (IgG) antibodies. Mechanically it plays a role in the immune response by recognizing and binding to specific antigens. Its molecular weight is approximately 146 kDa which is typical for IgG molecules. IgG4 is commonly expressed in the blood and extracellular fluids. It can also be found at sites of chronic inflammation reflecting its involvement in modulating immune responses.

Biological function summary

IgG4 antibodies act to regulate immune responses and prevent excessive inflammation. It does not engage in strong effector functions like other IgG subclasses due to its unique ability to exchange half-molecules leading to functional monovalency. This characteristic makes it part of an immune complex that can block other antibody functions by competitive binding without activating complement pathways. The IgG4 sequence shows unique properties that allow it to engage selectively in regulatory pathways which is a distinct attribute among immunoglobulins.

Pathways

IgG4 functions within immune regulatory pathways that inhibit inflammation. It involves pathways like the Fc gamma receptor-mediated suppression of effector cell responses. Through this pathway IgG4 can interact with proteins such as Fc gamma receptors on cells altering signaling cascades that prevent unnecessary immune activation. IgG4 antibodies play these roles alongside other immune-modulating proteins helping to balance immune system activity.

Associated diseases and disorders

IgG4 relates to conditions like IgG4-related disease (IgG4-RD) and autoimmune diseases where it presents an abnormal increase in serum levels and tissue infiltration. IgG4-RD is characterized by elevated IgG4 protein and causes fibrotic tissue inflammation in organs such as the pancreas and salivary glands. IgG4's interaction with proteins like T regulatory cells may contribute to downregulating immune attacks in autoimmune disease highlighting its dual role in both defensive and regulatory immune activities.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

Sandwich ELISA - Human IgG4 ELISA Kit (ab320788)
Example of human IgG4 standard curve in 1X Wash Buffer PT. Example of human IgG4 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human IgG4 ELISA Kit (ab320788)
Interpolated concentrations of human IgG4 in serum, plasma (citrate), plasma (EDTA), and plasma (heparin). Interpolated concentration of native IgG4 was measured in duplicate at different sample concentrations. Undiluted samples are 1:100,000 serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT.
Sandwich ELISA - Human IgG4 ELISA Kit (ab320788)
Interpolated concentrations of human IgG4 in urine, saliva, and milk. Interpolated concentration of native IgG4 was measured in duplicate at different sample concentrations. Undiluted samples are as follows: urine 1:20, saliva 1:100, and milk 1:100. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT.
Sandwich ELISA - Human IgG4 ELISA Kit (ab320788)
Interpolated concentrations of IgG4 in normal human serum donors. Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean IgG4 concentration was determined to be 248 ?g/mL with a range of 102 – 566 ?g/mL.
Sandwich ELISA - Human IgG4 ELISA Kit (ab320788)
Example of human IgG4 standard curve in 1X Wash Buffer PT in 96-well vs. 384-well plate. Example of human IgG4 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human IgG4 ELISA Kit (ab320788)
Interpolated concentrations of human IgG4 in serum in 96-well vs. 384-well plates. Interpolated concentration of native IgG4 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1:100,000 serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT.
Sandwich ELISA - Human IgG4 ELISA Kit (ab320788)
Cross reactivity of related immunoglobulin proteins. 50 ng/mL of natively purified human IgG1, IgG2, IgG3, IgA, IgD, IgE, and IgM were tested individually for cross-reactivity. Percent reactivity was determined by interpolating the protein concentration from the human IgG4 standard curve and expressing the interpolated concentration as a percentage of the protein concentration tested.