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AB214568

Human IgM ELISA Kit

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(6 Publications)

Human IgM ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IgM with a sensitivity of 50 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

View Alternative Names

Immunoglobulin heavy constant mu, Ig mu chain C region, Ig mu chain C region BOT, Ig mu chain C region GAL, Ig mu chain C region OU, IGHM

7 Images
ELISA - Human IgM ELISA Kit (AB214568)
  • ELISA

Supplier Data

ELISA - Human IgM ELISA Kit (AB214568)
Sandwich ELISA - Human IgM ELISA Kit (AB214568)
  • sELISA

Lab

Sandwich ELISA - Human IgM ELISA Kit (AB214568)

Example of human IgM standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IgM ELISA Kit (AB214568)
  • sELISA

Supplier Data

Sandwich ELISA - Human IgM ELISA Kit (AB214568)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IgM concentration was determined to be 524 μg/mL with a range of 178 – 901 μg/mL.

Sandwich ELISA - Human IgM ELISA Kit (AB214568)
  • sELISA

Supplier Data

Sandwich ELISA - Human IgM ELISA Kit (AB214568)

Interpolated concentrations of native IgM in human milk, saliva, and PBMC cell culture supernatant samples.

The concentrations of IgM were measured in duplicates, interpolated from the IgM standard curves and corrected for sample dilution. Undiluted samples are as follows : milk 1 : 750, saliva 1 : 400, and PBMC cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IgM concentration was determined to be 14,328 ng/mL in neat milk, 7,869 ng/mL in neat saliva and 16.0 ng/mL in neat PBMC cell culture supernatant.

Sandwich ELISA - Human IgM ELISA Kit (AB214568)
  • sELISA

Supplier Data

Sandwich ELISA - Human IgM ELISA Kit (AB214568)

Example of human IgM standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IgM ELISA Kit (AB214568)
  • sELISA

Supplier Data

Sandwich ELISA - Human IgM ELISA Kit (AB214568)

Interpolated concentrations of native IgM in human serum and plasma samples.

The concentrations of IgM were measured in duplicates, interpolated from the IgM standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 40,000, plasma (citrate) 1 : 40,000, plasma (EDTA) 1 : 40,000 and plasma (heparin) 1 : 40,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IgM concentration was determined to be 618.9 μg/mL in neat serum, 559.0 μg/mL in neat plasma (citrate), 705.1 μg/mL in neat plasma (EDTA), and 618.2 μg/mL in neat plasma (heparin).

Sandwich ELISA - Human IgM ELISA Kit (AB214568)
  • sELISA

Lab

Sandwich ELISA - Human IgM ELISA Kit (AB214568)

Interpolated concentration of native IgM was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 40,000 serum. The interpolated dilution factor corrected values to neat are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

Detection method

Colorimetric

Sample types

Saliva, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 50 pg/mL

Range

390.63 - 25000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human IgM ELISA Kit ab214568 is a rapid single-wash 90-min Sandwich ELISA to measure Human IgM in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, milk, saliva, serum. This SimpleStep sensitivity is 50 pg/mL.

How the assay works

Human IgM SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human IgM ELISA Kit ab214568 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our 384-well Human IgM ELISA Kit only requires a maximum of 12.5µL sample

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-IgM antibody [EPR20731] - BSA and Azide free (Capture) ab281109
- Anti-IgM antibody [EPR20731-259] - BSA and Azide free (Detector) ab281109

Immunoglobulin M, or IgM, is a basic antibody produced by B cells and the largest of the immunoglobulins in the Human circulatory system. IgM typically constitutes about 10% of serum immunoglobulins and is prominent in the early immune responses. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. IgM typically forms pentamers which includes the J chain, while in the less common hexameric form the J chain is absent. Although the common pentameric form of IgM contains 10 antigen binding sites, IgM cannot bind 10 antigens simultaneously due to size constraints. Class specific anti-immunoglobulin antibodies are useful for the characterization of malignant B cell proliferations. Most lymphocytic leukemias share either surface or intra cytoplasmic Ig with an isotypic restriction, which suggest the monoclonal nature of the cell population. Most of the chronic lymphocytic leukemias, non Hodgkin lymphomas and Burkitt's lymphoma bear surface IgM, whereas plasmocytes from Waldenstrõm's disease bear intracytoplasmic IgM.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.8" }, { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.9" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "94 - 105 %", "average": "= 100" }, { "sample": "Milk", "range": "90 - 101 %", "average": "= 96" }, { "sample": "Serum", "range": "93 - 98 %", "average": "= 96" }, { "sample": "EDTA Plasma", "range": "101 - 103 %", "average": "= 102" }, { "sample": "Saliva", "range": "94 - 110 %", "average": "= 101" }, { "sample": "Heparin Plasma", "range": "97 - 100 %", "average": "= 98" }, { "sample": "Citrate plasma", "range": "104 - 114 %", "average": "= 108" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IgM also known as immunoglobulin M is a type of antibody with a molecular mass of approximately 970 kDa. It is the largest antibody present in the human circulatory system mostly found in plasma and lymphatic tissue. IgM is secreted primarily by B cells as a pentamer with a J chain providing a high avidity for antigens. When bound to an antigen IgM can activate the complement system a part of the immune system that helps clear pathogens from an organism.
Biological function summary

IgM acts as the first line of defense during the initial stages of an immune response. It plays an important part in agglutination and neutralization of pathogens forming a complex that alerts other components of the immune system to take action. IgM exists before class-switching to IgG providing immediate protection. It also plays a role in antibody-dependent cellular cytotoxicity. Although not typically a long-term solution its presence is critical for providing initial protection until more specific antibodies like IgG and IgA are produced.

Pathways

The immune response that involves IgM begins with the activation of the classical complement pathway. This pathway bridges the innate and adaptive immunity ensuring the communication and coordination of immune responses. The pathway involves C1q protein binding to IgM-antigen complexes. IgM also interacts with antigen-presenting cells facilitating the processing of antigens and interaction with T cells. These interactions connect IgM with other proteins like IgG and IgA influencing various immune response pathways.

IgM is associated with conditions such as Waldenstrõm's macroglobulinemia and rheumatoid arthritis. Waldenstrõm's macroglobulinemia involves the overproduction of IgM by clonal B cells leading to symptoms like anemia bleeding and neuropathy. Rheumatoid arthritis shows increased IgM levels as part of the autoimmune response affecting joints. In these diseases the IgM antibody interacts with rheumatoid factor and other immune proteins involved in inflammation demonstrating its significant role in autoimmunity and abnormal immune responses.
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Product protocols

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Target data

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268).. Isoform 1. Constant region of secreted IgM (sIgM), also known as the Fc region of IgM antibody. Able to multimerize, forms high order polymers, mainly pentamers and occasionally hexamers, providing for multivalency and high avidity recognition of antigens (PubMed : 32029689, PubMed : 37095205). Natural sIgM are polyreactive and recognize conserved self- and pathogen-derived structures, whereas immune sIgM are secreted only upon exposure to pathogens and are antigen-specific. Both natural and immune sIgM are required for an efficient humoral immune response to infection (By similarity). Mediates sIgM effector functions mostly via Fc receptors and the complement system. On lymphoid cells binds high-affinity Fc receptor FCMR and promotes induction of an efficient neutralizing IgG response while maintaining tolerance to self-antigens. Recruits C1q complement component to initiate the classical complement pathway, facilitating the recognition and neutralization of pathogens by the host. Together with C1q and mannose-binding lectin promotes the phagocytosis of apoptotic cells by macrophages, ensuring the clearance of potential autoimmune epitopes from tissues (By similarity) (PubMed : 12847249, PubMed : 19006321, PubMed : 28230186, PubMed : 32029689). Involved in mucosal immunity. It is transported by transcytosis across mucosal epithelium by PIGR and secreted on the apical side in complex with PIGR secretory component to scan mucosal lining for pathogens. IgM-antigen complexes undergo FCMR-mediated retrotranscytosis across mucosal M cells toward antigen-presenting cells in mucosal lymphoid tissues (By similarity) (PubMed : 32029689).. Isoform 2. Constant region of membrane-bound IgM, part of the B cell receptor complex (BCR). IgM BCR provides constitutive tonic signaling for B cell survival. Mediates pre-BCR signaling that regulates B cell selection and rearrangement of Ig genes via allelic exclusion.
See full target information IGHM
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of inflammation (London, England) 21:30 PubMed39135051

2024

Evidence of aberrant anti-epstein-barr virus antibody response, though no viral reactivation, in people with post-stroke fatigue.

Applications

Unspecified application

Species

Unspecified reactive species

Isobel C Mouat,Li Zhu,Alperen Aslan,Barry W McColl,Stuart M Allan,Craig J Smith,Marion S Buckwalter,Laura McCulloch

Heliyon 10:e30556 PubMed38770298

2024

Biological changes in human B-cell line Ramos (RA.1) related to increasing doses of human parathyroid hormone.

Applications

Unspecified application

Species

Unspecified reactive species

Lady J Rios-Serna,Angie M Rosero,Gabriel J Tobón,Carlos A Cañas

American journal of translational research 14:918-926 PubMed35273695

2022

Ulinastatin combined with glutamine improves liver function and inflammatory response in patients with severe acute pancreatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Li Zhao,Yanrong Ma,Qiuyue Li,Yuhan Wang

Antioxidants (Basel, Switzerland) 10: PubMed33919749

2021

Oxidative Stress and Inflammatory Markers in Abdominal Aortic Aneurysm.

Applications

Unspecified application

Species

Unspecified reactive species

David Sánchez-Infantes,Meritxell Nus,Miquel Navas-Madroñal,Joan Fité,Belén Pérez,Antonio J Barros-Membrilla,Begoña Soto,José Martínez-González,Mercedes Camacho,Cristina Rodriguez,Ziad Mallat,María Galán

Polish journal of microbiology 68:51-57 PubMed31050253

2019

Clinical Interpretation of Detection of IgM Anti- Antibody in the Absence of IgG and ; a Diagnostic Challenge for Clinicians.

Applications

Unspecified application

Species

Unspecified reactive species

Reem Al Jindan,Nida Saleem,Aamir Shafi,Sheikh Muhammad Amjad

Clinical and experimental pharmacology & physiology 44:556-565 PubMed28222218

2017

Reduced inflammatory responses of follicular helper T cell promote the development of regulatory B cells after Roux-en-Y gastric bypass.

Applications

Unspecified application

Species

Unspecified reactive species

Junfang Zhan,Liyu Huang,Haiyong Ma,Huan Chen,Yuan Yang,Sheng Tan,Wendy Song,Weiguo Zhao,Xiaojiang Dai
View all publications
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