AB314733
Human IkB alpha ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human IkB alpha in Human in Biofluids, Cell Culture Media samples.
View Alternative Names
IKBA, MAD3, NFKBI, NFKBIA, NF-kappa-B inhibitor alpha, I-kappa-B-alpha, Major histocompatibility complex enhancer-binding protein MAD3, IkB-alpha, IkappaBalpha
1 Images
- sELISA
Supplier Data
Sandwich ELISA - Human IkB alpha ELISA Kit (AB314733)
Example of standard curve using ab314733. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.
Reactivity data
{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
Product details
Human IkB alpha ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IkB alpha in serum (human IkB alpha concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants. This assay employs an antibody specific for human IkB-alpha coated on a 96-well plate.
Recovery
[ { "sample": "Serum", "range": "84 - 103 %", "average": "= 97.18" }, { "sample": "Plasma", "range": "94 - 107 %", "average": "= 101.7" }, { "sample": "Cell culture media", "range": "119 - 145 %", "average": "= 133.3" } ]
What's included?
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Properties and storage information
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL (RELA/p65 and NFKB1/p50) dimers in the cytoplasm by masking their nuclear localization signals (PubMed : 1493333, PubMed : 36651806, PubMed : 7479976). On cellular stimulation by immune and pro-inflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription (PubMed : 7479976, PubMed : 7628694, PubMed : 7796813, PubMed : 7878466).
Complementary Products
Primary Antibodies
AB32518
Anti-IKB alpha antibody [E130]
20ul selling size|RabMAb|Recombinant|KO Validated
![Western blot - Anti-IKB alpha antibody [E130] (AB32518)](https://content.abcam.com/products/images/ikb-alpha-antibody-e130-ab32518--western-blot-img54191.jpg)
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Product promise
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