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Human IkB alpha ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human IkB alpha in Human in Serum, Plasma, Cell culture supernatant samples.

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Images

Sandwich ELISA - Human IkB alpha ELISA Kit (AB314733), expandable thumbnail

Key facts

Detection method
Colorimetric
Sample types
Serum, Plasma, Cell culture supernatant
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
0.65 - 150 ng/mL
Sensitivity
= 0.65 ng/mL

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Target data

Function

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL (RELA/p65 and NFKB1/p50) dimers in the cytoplasm by masking their nuclear localization signals (PubMed:1493333, PubMed:36651806, PubMed:7479976). On cellular stimulation by immune and pro-inflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription (PubMed:7479976, PubMed:7628694, PubMed:7796813, PubMed:7878466).

Alternative names

IKBA, MAD3, NFKBI, NFKBIA, NF-kappa-B inhibitor alpha, I-kappa-B-alpha, Major histocompatibility complex enhancer-binding protein MAD3, IkB-alpha, IkappaBalpha

What's included?

1 x 96 Tests
Components
20X Wash Buffer
1 x 25 mL
5X Assay Diluent (Item E2)
1 x 15 mL
600X HRP-Streptavidin
1 x 200 µL
96-Well Plate Sealing Film
1 x 1 Unit
Biotinylated Human IkB alpha Detection Antibody
2 x 1 Vial
Human IkB alpha Antibody-coated ELISA Plate
1 x 1 Unit
Lyophilized Human IkB alpha Protein Standard
2 x 1 Vial
Stop Solution; 0.2M Sulfuric Acid
1 x 8 mL
TMB One-Step Substrate Reagent
1 x 12 mL

Recommended products

Human IkB alpha ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human IkB alpha in Human in Serum, Plasma, Cell culture supernatant samples.

Key facts

Detection method
Colorimetric
Sample types
Serum, Plasma, Cell culture supernatant
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
0.65 - 150 ng/mL
Assay Platform
Pre-coated microplate (12 x 8 well strips)
Sensitivity
= 0.65 ng/mL

Recovery

Sample specific recovery

Sample type
Serum
Average %
= 97.18
Range
84 - 103 %
Sample type
Plasma
Average %
= 101.7
Range
94 - 107 %
Sample type
Cell culture media
Average %
= 133.3
Range
119 - 145 %

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Notes

Human IkB alpha ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IkB alpha in serum (human IkB alpha concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants. This assay employs an antibody specific for human IkB-alpha coated on a 96-well plate. 

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Human IkB alpha ELISA Kit (ab314733), expandable thumbnail

    Sandwich ELISA - Human IkB alpha ELISA Kit (ab314733)

    Example of standard curve using ab314733. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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