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AB313974

Human IKB beta ELISA Kit

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Human IKB beta ELISA Kit is a Sandwich ELISA for the measurement of Human IKB beta in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

IKBB, TRIP9, NFKBIB, NF-kappa-B inhibitor beta, NF-kappa-BIB, I-kappa-B-beta, Thyroid receptor-interacting protein 9, IkB-B, IkB-beta, IkappaBbeta, TR-interacting protein 9, TRIP-9

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Sandwich ELISA - Human IKB beta ELISA Kit (AB313974)
  • sELISA

Supplier Data

Sandwich ELISA - Human IKB beta ELISA Kit (AB313974)

Example of standard curve using ab313974. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.55 ng/mL

Range

0.55 - 500 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Product details

Human IKB beta ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IKB beta in serum, plasma (collect plasma using EDTA and Heparin as an anticoagulant. Citrate is not recommended in this assay), and cell culture supernatants. This assay employs an antibody specific for human IKB beta coated on a 96-well plate.

Recovery

[ { "sample": "Serum", "range": "107 - 136 %", "average": "= 120.7" }, { "sample": "Plasma", "range": "71 - 82 %", "average": "= 75.12" }, { "sample": "Cell culture media", "range": "126 - 140 %", "average": "= 133.4" } ]

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313974-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB313974-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313974-CMP07", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313974-CMP06", "productcode":"" }, { "size":"1 x 200 µL", "name":"350X HRP-Streptavidin", "number":"AB313974-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human IKB beta Antibody-coated ELISA Plate", "number":"AB313974-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human IKB beta Protein Standard", "number":"AB313974-CMP10", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313974-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human IKB beta Detection Antibody", "number":"AB313974-CMP09", "productcode":"" } ] } } }

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C

Product protocols

Target data

Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further NFKBIA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.
See full target information NFKBIB
websiteProtocolBooklet
en

Product promise

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