Human IL-1 alpha ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human IL-1 alpha in Human in Cell culture extracts, Tissue Extracts samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.82 - 600 pg/mL
- Sensitivity
- < 2 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Cytokine constitutively present intracellularly in nearly all resting non-hematopoietic cells that plays an important role in inflammation and bridges the innate and adaptive immune systems (PubMed:26439902). After binding to its receptor IL1R1 together with its accessory protein IL1RAP, forms the high affinity interleukin-1 receptor complex (PubMed:17507369, PubMed:2950091). Signaling involves the recruitment of adapter molecules such as MYD88, IRAK1 or IRAK4 (PubMed:17507369). In turn, mediates the activation of NF-kappa-B and the three MAPK pathways p38, p42/p44 and JNK pathways (PubMed:14687581). Within the cell, acts as an alarmin and cell death results in its liberation in the extracellular space after disruption of the cell membrane to induce inflammation and alert the host to injury or damage (PubMed:15679580). In addition to its role as a danger signal, which occurs when the cytokine is passively released by cell necrosis, directly senses DNA damage and acts as a signal for genotoxic stress without loss of cell integrity (PubMed:26439902).
Alternative names
IL1F1, IL1A, Interleukin-1 alpha, IL-1 alpha, Hematopoietin-1
What's included?
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Human IL-1 alpha ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human IL-1 alpha in Human in Cell culture extracts, Tissue Extracts samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture extracts, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.82 - 600 pg/mL
- Assay Platform
- Microplate
- Sensitivity
- < 2 pg/mL
- General recovery
- 95%
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture extracts | Average % = 94.78 | Range 96 - 106 % |
Sample type Tissue Extracts | Average % = 95.56 | Range 85 - 105 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Abcam's Human IL-1 alpha ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-1 alpha in cell lysates and tissue lysates.
This assay employs an antibody specific for Human IL-1 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of
IL-1 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get higher sensitivity in only 90 minutes with Human IL-1a ELISA Kit (Human IL-1a ELISA Kit ab178008) from our SimpleStep ELISA® range.
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Sandwich ELISA - Human IL-1 alpha ELISA Kit (ab100561)
Representative standard curve using ab100561
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Product protocols
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