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AB46028

Human IL-1 alpha ELISA Kit

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(1 Publication)

Human IL-1 alpha ELISA Kit is a Sandwich ELISA for the measurement of Human IL-1 alpha in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

IL1F1, IL1A, Interleukin-1 alpha, IL-1 alpha, Hematopoietin-1

1 Images
Sandwich ELISA - Human IL-1 alpha ELISA Kit (AB46028)
  • sELISA

Unknown

Sandwich ELISA - Human IL-1 alpha ELISA Kit (AB46028)

Representative Standard Curve using ab46028

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 10 pg/mL

Range

31.25 - 1000 pg/mL

Assay time

3h 45m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's IL-1 alpha Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-1 alpha in cell culture supernatants, buffered solutions, serum, plasma and other body fluids. This assay will recognize both natural and recombinant Human IL-1a.

A monoclonal antibody specific for IL-1 alpha has been coated to the wells of the microtiter strip plate provided. Samples, including standards of known IL-1 alpha concentrations and unknown are pipetted into these wells. During incubation, binding of IL-1 alpha to the capture antibodies is completed and then any excess unbound analyte is removed by washing. A biotinylated monoclonal antibody specific for IL-1 alpha is then incubated. After washing, the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-1 alpha present in the sample.

This kit will recognize both endogenous and recombinant Human IL-1 alpha.

Get higher sensitivity in only 90 minutes with Human IL-1 alpha ELISA Kit (ab178008) from our SimpleStep ELISA® range.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample A", "replicates": 8, "mean": "259.9 pg/mL", "standardDeviation": "15", "coefficientOfVariability": "5.8" }, { "reproducibilityType": "Inter", "sample": "Sample B", "replicates": 8, "mean": "58.6 pg/mL", "standardDeviation": "4.3", "coefficientOfVariability": "7.3" }, { "reproducibilityType": "Intra", "sample": "Sample B", "replicates": 21, "mean": "72.6 pg/mL", "standardDeviation": "3.9", "coefficientOfVariability": "4.3" }, { "reproducibilityType": "Intra", "sample": "Sample A", "replicates": 22, "mean": "459.8 pg/mL", "standardDeviation": "13", "coefficientOfVariability": "2.8" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Cytokine constitutively present intracellularly in nearly all resting non-hematopoietic cells that plays an important role in inflammation and bridges the innate and adaptive immune systems (PubMed : 26439902). After binding to its receptor IL1R1 together with its accessory protein IL1RAP, forms the high affinity interleukin-1 receptor complex (PubMed : 17507369, PubMed : 2950091). Signaling involves the recruitment of adapter molecules such as MYD88, IRAK1 or IRAK4 (PubMed : 17507369). In turn, mediates the activation of NF-kappa-B and the three MAPK pathways p38, p42/p44 and JNK pathways (PubMed : 14687581). Within the cell, acts as an alarmin and cell death results in its liberation in the extracellular space after disruption of the cell membrane to induce inflammation and alert the host to injury or damage (PubMed : 15679580). In addition to its role as a danger signal, which occurs when the cytokine is passively released by cell necrosis, directly senses DNA damage and acts as a signal for genotoxic stress without loss of cell integrity (PubMed : 26439902).
See full target information IL1A
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Tohoku journal of experimental medicine 237:183-91 PubMed26477692

2015

Blueberry Improves the Therapeutic Effect of Etanercept on Patients with Juvenile Idiopathic Arthritis: Phase III Study.

Applications

Unspecified application

Species

Unspecified reactive species

Yingjie Zhong,Ye Wang,Jun Guo,Haifeng Chu,Yong Gao,Limin Pang
View all publications
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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