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AB47350

Human IL-1 alpha ELISA Set (without plates)

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Human IL-1 alpha ELISA Set (without plates) is a Sandwich (quantitative) ELISA for the measurement of Human IL-1 alpha ELISA Set (without plates) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

IL1F1, IL1A, Interleukin-1 alpha, IL-1 alpha, Hematopoietin-1

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Sandwich ELISA - Human IL-1 alpha ELISA Set (without plates) (AB47350)
  • sELISA

Supplier Data

Sandwich ELISA - Human IL-1 alpha ELISA Set (without plates) (AB47350)

Key facts

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 10 pg/mL

Range

31.25 - 1000 pg/mL

Assay time

3h 45m

Assay Platform

Reagents

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's IL-1α Human in vitro ELISA Set is designed for the quantitative measurement of IL-1α in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

This ELISA set is intended to measure human IL-1 alpha in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.

The IL-1 alpha ELISA set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

Recognizes both natural and recombinant human IL-1a.

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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Cytokine constitutively present intracellularly in nearly all resting non-hematopoietic cells that plays an important role in inflammation and bridges the innate and adaptive immune systems (PubMed : 26439902). After binding to its receptor IL1R1 together with its accessory protein IL1RAP, forms the high affinity interleukin-1 receptor complex (PubMed : 17507369, PubMed : 2950091). Signaling involves the recruitment of adapter molecules such as MYD88, IRAK1 or IRAK4 (PubMed : 17507369). In turn, mediates the activation of NF-kappa-B and the three MAPK pathways p38, p42/p44 and JNK pathways (PubMed : 14687581). Within the cell, acts as an alarmin and cell death results in its liberation in the extracellular space after disruption of the cell membrane to induce inflammation and alert the host to injury or damage (PubMed : 15679580). In addition to its role as a danger signal, which occurs when the cytokine is passively released by cell necrosis, directly senses DNA damage and acts as a signal for genotoxic stress without loss of cell integrity (PubMed : 26439902).
See full target information IL1A
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